Characterization of polysaccharides extracted from Platycodon grandiflorus (Jacq.) A.DC. affecting activation of chicken peritoneal macrophages

Zheng, Pimiao; Fan, Wentao; Wang, Shenghua; Pān, Hào; Wang, Yang; Wan, Huiyu; Hao, Zhihui; Liu, Jianzhu; Zhao, Xiaona

doi:10.1016/j.ijbiomac.2016.12.077

Cited by 53 publications

(21 citation statements)

References 51 publications

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“…Similarly, the polysaccharides ASP (46), KMCP (11), SF1, SF2 (47), SPS (48), SBF (49), PG (50), and SHE (51) can stimulate NO release in mouse peritoneal macrophages and/or macrophage RAW264.7, in which SBF inhibits the release of NO in LPS-activated macrophages as well as the release of cytokines and activity mediators (49). Polysaccharides GG, SGG, and BPs inhibit the release of NO in LPS-activated macrophages RAW264.7, which suggests that these polysaccharides have similar immunomodulatory activity (36, 45).…”

Section: Immunomodulatory Effects Of Plant Polysaccharidesmentioning

confidence: 99%

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

2019

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Polysaccharides are among the most important members of the biopolymer family. They are natural macromolecules composed of monosaccharides. To date, more than 300 kinds of natural polysaccharide compounds have been identified. They are present in plants, animals, and microorganisms, and they engage in a variety of physiological functions. In the 1950s, due to the discovery of their immunoregulatory and anti-tumor activities, polysaccharides became a popular topic of research in pharmacology, especially in immunopharmacology. Plants are an important source of natural polysaccharides. Pharmacological and clinical studies have shown that plant polysaccharides have many functions, such as immune regulation, anti-tumor activity, anti-inflammatory activity, anti-viral functions, anti-radiation functions, and a hypoglycaemic effect. The immunomodulatory effects of plant polysaccharides have received much attention. Polysaccharides with these effects are also referred to as biological response modifiers (BRMs), and research on them is one of the most active areas of polysaccharide research. Thus, we summarize immunomodulatory effects of botanical polysaccharides isolated from different species of plants on the macrophage. The primary effect of botanical polysaccharides is to enhance and/or activate macrophage immune responses, including increasing reactive oxygen species (ROS) production, and enhancing secretion of cytokines and chemokines. Therefore, it is believed that botanical polysaccharides have significant therapeutic potential, and represent a new method for discovery and development of novel immunomodulatory medicine.

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Section: Immunomodulatory Effects Of Plant Polysaccharidesmentioning

confidence: 99%

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

2019

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“…Platycodon grandifloras (PG) is widely cultivated in East Asia and Eastern Siberia, as an ornamental plant and traditional herb medicine, has been used as a health food and medicine for the treatment of cough, chest tightness, bronchitis, pleurisy, etc. ( Zhang et al, 2015 ; Zheng et al, 2017 ). The research on PG mainly focused on its biological activities, including its anti-tumor, anti-oxidation, and immune-enhancing effects.…”

Section: Introductionmentioning

confidence: 99%

“…The research on PG mainly focused on its biological activities, including its anti-tumor, anti-oxidation, and immune-enhancing effects. PG polysaccharides have immunostimulatory activity ( Zheng et al, 2017 ), induce DC maturation and increase the expression levels of MHC-I/II, co-stimulatory molecules and cytokines, which can activate T cells ( Park et al, 2014 ; Zheng et al, 2017 ). However, the effects of PG polysaccharides on the distribution of GA have remained elusive.…”

Section: Introductionmentioning

confidence: 99%

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

et al. 2018

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Purpose: Understanding the distribution and interaction of the Traditional Chinese Medicines (TCMs) is an integral source of herbal drug discovery. An optimized radio-labeled method was explored that could conduct in situ biodistribution studies in animals. We evaluated the feasibility of the method and applied glycyrrhetinic acid and platycodon (PG) polysaccharides as models.Procedures: [18F]-GA is a novel radiotracer which was performed positron emission tomography (PET) studies to assay the biodistribution of GA in mice. In addition, PG polysaccharide was used to intervene the biodistribution and dosimetry of GA. Scanning data were analyzed with professional software.Results: Record the time-activity curves for all organs then use the normalization method to calculate the area under the curve as a dosimetry for each organ. Moreover, the addition of PG polysaccharides can significantly improve the dosimetry of GA in the lungs, and its effect was related to the administration time.Conclusion: MicroPET imaging opens up a new avenue for the application of drug interactions between the TCMs.

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“…The structure identification of PGPSs was assessed in our previous research [ 11 ]. FT-IR analysis showed that PGPSs possess typical absorption peak of polysaccharides.…”

Section: Resultsmentioning

confidence: 99%

“…The carbohydrate contents of PGPS tc , PGPS 60c , PGPS 80c and PGPS tp were 64%, 53.7%, 63.5% and 73%, respectively, as measured by a phenol hydrate-sulfuric acid method using analytical grade glucose as a standard sample [ 10 ]. The structure identification of PGPSs was assessed in our previous study [ 11 ].…”

Section: Methodsmentioning

confidence: 99%

Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro

et al. 2017

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The study is aimed at investigating the immunoenhancement activity of polysaccharides from Platycodon grandiflorum polysaccharides (PGPSs) in vitro. In this study, some research on lymphocyte proliferation, cell cycle, and the levels of CD4+ and CD8+ T cells were performed. Four different concentrations of PGPSs (PGPStc, PGPS60c, PGPS80c, and PGPStp) were harvested and added to peripheral blood T lymphocytes. We observed significant increases in T lymphocyte proliferation at PGPStc groups individually or synergistically with phytohemagglutinin (PHA) at most concentrations, and their lymphocyte proliferation rates were the highest. The active sites of PGPStc and PGPS60c were subsequently chosen. Then, we utilized flow cytometry to determine lymphocyte cell cycle distribution and levels of CD4+ and CD8+ T cells. At most time points, PGPStc could facilitate lymphocyte cell cycle progression from the G0/G1 phase to the S and G2/M phases and, simultaneously, increase the levels of CD4+ and CD8+ T cells. These results indicate that PGPStc enhances the immune functions, suggesting that PGPStc could be a potential immunopotentiator for further in vivo and clinical trial experiments.

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Characterization of polysaccharides extracted from Platycodon grandiflorus (Jacq.) A.DC. affecting activation of chicken peritoneal macrophages

Cited by 53 publications

References 51 publications

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

Advances in Research on Immunoregulation of Macrophages by Plant Polysaccharides

An Optimized MicroPET Imaging Method for the Distribution and Synergies of Natural Products

Effects of Polysaccharides from Platycodon grandiflorum on Immunity-Enhancing Activity In Vitro

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