Characterization of Quince (Cydonia) Cultivars Using Polyacrylamide Gel Electrophoresis

Sánchez, Enrique Eduardo; Menendez, Ricardo; Daley, L. S.; Boone, R.B.; Jahn, Otto L.; Lombard, P. B.

doi:10.24266/0738-2898-6.2.53

Cited by 8 publications

(3 citation statements)

References 15 publications

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“…Therefore, genotypes located closer to the ideal genotype are more desirable than others; thus, genotype Svs1 was more desirable followed by genotypes Nb4, Esf and Et1 while genotypes Nb1, Kvd3 and Svs2 were the most unfavorable and genotype Kvd2 was, of course, the poorest genotype because it was consistently the poorest (Figure 3). Sanchez et al (1988) studied 11 quince genotypes by isozyme patterns which were considered to represent genetic characteristics suitable for identification purposes but the diversity of isozyme patterns was much less than other markers while in the present study using morphologic traits and biplot method could detect the best genotype. The biplot in Figure 4 is used for visualizing the relationships among the genotypes and the lines connecting the biplot origin with the markers for the genotypes are called genotype vectors.…”

Section: Resultsmentioning

confidence: 81%

Evaluation of Some Traits in Local Iranian Quince (Cydonia OblongaMiller) Genotypes

Rahimi

Asadi-Gharneh

Sabaghnia

2018

International Journal of Fruit Science

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Quince (Cydonia oblonga Miller.) belongs to the Rosaceae family and is generally used for fresh consumption and processed items. In this research, fruit characteristics of eight quince genotypes from Iran were investigated via genotype by trait interactions (GTI) biplot. Results indicated that the first two principal components analysis (PC1 and PC2) were used to create a two-dimensional GTI biplot that accounted percentages of 31% and 22%, respectively, of sums of squares of the GTI. The vertex genotypes in polygon were Svs1, Esf, Nb1, Kud2 and Et1; and Svs1 was the winning genotype for pectin, fruit length, phosphorus percent and fruit hardness while Esf was the best genotype for iron percent, fruit diameter, fruit weight and end hold. There were positive correlation among pectin, fruit length, fruit hardness and phosphorus percent as well as among zinc percent, branch length, fruit apparent specific weight, calcium percent and potassium percent. Genotype Svs1 was more desirable followed by genotypes Nb4, Esf and Et1 based on ideal entry biplot and pectin and fruit length were the best traits according to ideal tester biplot. The best genotype for obtaining of high fruit weight was Esf following to Nb4, Svs1 and Nb1 and ranking traits based on fruit weight showed that fruit diameter is more important followed by end hold and iron percent. This study showed that there was relatively high level of variation in quince genotypes grown and morphological traits used in this research indicating that they can be used for characterization and diversity analysis of quince. We concluded from this study that it is imperative for breeders to pay more attention to improve quince morphological traits coordinated to fruit yield performance.

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Section: Resultsmentioning

confidence: 81%

Evaluation of Some Traits in Local Iranian Quince (Cydonia OblongaMiller) Genotypes

Rahimi

Asadi-Gharneh

Sabaghnia

2018

International Journal of Fruit Science

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“…Probably 'Esme' name may be a mixed population instead of one cultivar. Sanchez et al, (1988) reported that 'Tekes' and 'Limon' cultivars had same isozyme pattern. In our study, this two cultivars were also closely related.…”

Section: Molecular Analysismentioning

confidence: 99%

Morphological and Molecular Characterization of Major Quince Cultivars from Turkey

Pınar

Kaymak

OZONGUN

et al. 2016

Not Bot Hort Agrobot Cluj

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Quince (Cydonia oblonga Mill.) belongs to the Rosaceae family is native to south-eastern Europa and Asia Minor. It is generally used for table consumption and processed into jam, jelly and marmalade. It is also used as dwarfing rootstock for pear cultivars. In the present study, fruit characteristics and genetic diversity of 17 quince cultivars from Turkey were investigated. For fruit characteristics, 'Bardacik' had the highest fruit weight. The highest SSC/Acidity ratio were obtained from 'Osmancik'. There was high level of variation in fruit characteristics among cultivars. In molecular analysis, totally 133 bands were obtained from 23 sequence-related amplified polymorphism (SRAP) primer combinations and 67 of them were polymorphic. Genetic similarity of 17 cultivars was between 0.78 and 1.00. Two cultivars ('Sekergevrek' and '32S04') were found to be more distinct from the other cultivars genetically. This study showed that there was low level of genetic variation most of quince cultivars grown in Turkey. SRAP markers firstly used in quince with this study indicating that it can be used for characterization and diversity analysis of quince.

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“…Studies on molecular characterization of quince accessions are limited in the literature (Sanchez, 1988;Yamamoto et al, 2004;Dumanoglu et al, 2009;Bayazit et al, 2011;Azad et al, 2013;Yuksel et al, 2013;Topcu et al, 2015;Pınar et al, 2016). The SSR technique has several advantages, such as high polymorphism, codominance, and reproducibility.…”

Section: Ssr Polymorphismmentioning

confidence: 99%

Characterization of quince (Cydonia oblonga Mill.) accessions by simple sequence repeat markers

Güney¹,

Kafkas²,

Koç³

et al. 2019

Turk J Agric For

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Introduction Quince (Cydonia oblonga Mill.) is a pome fruit, like apple and pear, that belongs to the family Rosaceae. It originated in northern Iran, the Hazar Sea, South Caucasus, Khurasan, and Anatolia. Its fruit is mainly used by the food industry to produce jam, jelly, and marmalade. Quince is a good source of minerals, vitamins (especially vitamin C), and sugars (Bucsek et al., 1996) as well as flavonoid compounds, such as quercetin, rutin, and kaempferol (Silva et al., 2002, 2005). The total global production of quince has reached about 677,949 metric tons, and Turkey is one of the main producer countries, along with Uzbekistan and China, and is responsible for about 19% of the total global production (Faostat, 2016). In Turkey, most of the quince cultivars originated from seedlings accidentally grown in backyards or at the borders of orchards (Özbek, 1978). There are still many natural quince accessions in different regions of Turkey that are valuable genetic resources for quince breeding. All quince cultivars and accessions belong to one species in the genus Cydonia. Identification and characterization of quince accessions morphologically are considerably difficult due to high similarities in the tree structure and fruit traits of quince plants (Yamamoto et al., 2004). Identification of relationships based on morphological characteristics has been widely used in many species including walnut (Keles et al., 2014), cherry (Rakonjac et al., 2010), and olive (Cantini et al., 1999). However, morphological characterization does not perfectly reveal the relationship due to the influence of environmental factors and low heritability (Cadee, 2000; Bucheyeki et al., 2009). DNA-based molecular markers have been effective tools to characterize plant materials for the last several decades (Lacis et al., 2009). Simple sequence repeats (SSRs) are a marker of choice due to their codominant nature, abundance in the genome, suitability for automation, high polymorphism, and repeatability (Kacem et al., 2017). One of the main advantages of SSR markers is their transferability between closely related species (Schlotterer and Tautz, 1992). The use of SSR markers for molecular characterization is well proven in different species, such as apple (Gasi et al., 2016), apricot (Hormaza, 2002), peach (Bouhadida et al., 2007), pear (Fan et al., 2013), pistachio (Zaloglu et al., 2015), and walnut (Topcu et al., 2015). Therefore, the use of SSR markers to determine the relationships among quince accessions can be highly reliable.

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Characterization of Quince (Cydonia) Cultivars Using Polyacrylamide Gel Electrophoresis

Cited by 8 publications

References 15 publications

Evaluation of Some Traits in Local Iranian Quince (Cydonia OblongaMiller) Genotypes

Evaluation of Some Traits in Local Iranian Quince (Cydonia OblongaMiller) Genotypes

Morphological and Molecular Characterization of Major Quince Cultivars from Turkey

Characterization of quince (Cydonia oblonga Mill.) accessions by simple sequence repeat markers

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