Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes

Bumunang, Emmanuel W.; Babalola, Olubukola Oluranti

doi:10.1590/s1516-89132014000100001

Cited by 33 publications

(16 citation statements)

References 36 publications

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“…Clinical isolates of these control strains were tested in the same setup as P. aeruginosa , but no HCN could be detected. The findings were in accordance with Bumunang & Babalola32.…”

Section: Resultssupporting

confidence: 90%

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

Lauridsen

Sommer

Johansen

et al. 2017

Sci Rep

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Pseudomonas aeruginosa is the primary cause of chronic airway infections in cystic fibrosis (CF) patients. Persistent infections are seen from the first P. aeruginosa culture in about 75% of young CF patients, and it is important to discover new ways to detect P. aeruginosa at an earlier stage. The P. aeruginosa biomarker hydrogen cyanide (HCN) contains a triple bond, which is utilized in this study because of the resulting characteristic C≡N peak at 2135 cm−1 in a Raman spectrum. The Raman signal was enhanced by surface-enhanced Raman spectroscopy (SERS) on a Au-coated SERS substrate. After long-term infection, a mutation in the patho-adaptive lasR gene can alter the expression of HCN, which is why it is sometimes not possible to detect HCN in the breath of chronically infected patients. Four P. aeruginosa reference strains and 12 clinical P. aeruginosa strains isolated from CF children were evaluated, and HCN was clearly detected from overnight cultures of all wild type-like isolates and half of the later isolates from the same patients. The clinical impact could be that P. aeruginosa infections could be detected at an earlier stage, because daily breath sampling with an immediate output could be possible with a point-of-care SERS device.

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“…Clinical isolates of these control strains were tested in the same setup as P. aeruginosa , but no HCN could be detected. The findings were in accordance with Bumunang & Babalola32.…”

Section: Resultssupporting

confidence: 90%

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

Lauridsen

Sommer

Johansen

et al. 2017

Sci Rep

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“…Manuscript to be reviewed PGPR produce catalase enzyme (Mumtaz et al, 2017) that helps plants to withstand under stressed conditions by neutralizing the effect of hydrogen peroxide. In present study, all tested isolates were catalase positive, which is correlated with previous work as described by Chaiharn et al (2008) and Bumunang and Babalola (2014). Similarly, Shruti et al 2013 2011observed that out of ten strains, only five were urease positive.…”

Section: Discussionsupporting

confidence: 92%

“…Out of 20 isolates, seven isolates gave positive results for phosphate solubilization. These findings are in accordance with the results of Bumunang and Babalola (2014). Similarly, Hussain et al (2013) found 32 bacterial isolates with phosphorus solubilizing activity.…”

Section: Discussionsupporting

confidence: 89%

Peer Review #1 of "Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions (v0.1)"

Boon¹

2018

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Background: Low phosphorus availability limits crop production in alkaline calcareous soils in semi-arid regions including Pakistan. Phosphate solubilizing bacteria may improve crop growth on alkaline calcareous soils due to their ability to enhance P availability. Methods: Twenty rhizobacterial isolates (Q1-Q20) were isolated from rhizosphere of cotton and characterized for their growth promoting attributes in vitro. The selected phosphate solubilizing isolates were further screened for their ability to improve cotton growth under axenic conditions (jar trial). The phosphorus solubilization capacities of selected strains were quantified and these strains were identified through 16S rDNA sequencing. Results: Isolates Q2, Q3, Q6, Q7, Q8, Q13 and Q14 were able to solubilize phosphate from insoluble sources. Most of these isolates also possessed other traits including catalase activity and ammonia production. The growth promotion assay showed that Q3 was significantly better than most of the other isolates followed by Q6. Maximum root colonization (4.34 × 10 6 cfu g-1) was observed in case of isolate Q6 followed by Q3. The phosphorus solubilization capacities of these strains were quantified, showing a maximum phosphorus solubilization by Q3 (Optical density 2.605 ± 0.06) followed by the Q6 strain. The strain Q3 was identified as Bacillus subtilis (Accession # KX788864) and Q6 as Paenibacillus sp. (Accession # KX788865) through 16S rDNA sequencing. Discussion: The bacterial isolates varied in their abilities for different growth promoting traits. The selected PGPR Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 have multifarious growth promoting traits including ability to grow at higher EC and pH levels, and phosphorus solubilizing ability. These strains can efficiently colonize cotton roots under salt affected soils and help plants in phosphorus nutrition. It is concluded that both strains are potential

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“…Genus Sphingomonas discovered and presented the physiology and ecology by White et al [43] and Ali et al [44] reported genus Sphingomonas has a ability of nitrogen fixation, phosphate and potassium solubilization and Sphingomonas together with Burkholderia, Bacillus, Pseudomonas have been used biofertilizing microbes. Recently Bumunang and Babalota [45] examined the rhizobacteria from field grown GM maize in South Africa as follows species of Pseudomonas, Aeromonas, Sphingomonas, Burkholderia, Bacillus, Stenotrophomonas, Achromobacter, Ewingella, they have catalase activity, ammonia production, IAA production, phosphate solubilisation and antifungal activity. Our results also found the rhizospheric bacteria in acrisols and ferrasols with species of bacilli, betaproteobacteria as Burkholderia, Acidobacteria and Sphingomonas.…”

Section: Resultsmentioning

confidence: 99%

Isolation and Identification of Rhizospheric Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Acrisols and Ferrasols of Dong Nai Province, the Southeast of Vietnam

Tam¹,

Diep

2015

AJLS

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Nitrogen-fixing and phosphate-solubilizing bacterial diversity and population dynamics in the Acrisol and Ferrasol rhizosphere of sugarcane grown in Dong Nai province, the eastern of South Vietnam was studied. Soil rhizosphere samples were taken in three districts (sites) of this region. Physical and chemical characteristics of soil samples and total nitrogen-fixing and phosphate-solubilizing bacteria counts were determined by drop plate count method together with 16S rRNA gene fragments amplified from DNA using eubacterial universal primers (27F and 1492R). A total of 31 isolates were isolated on two media (Burk's N-free and NBRIP) and all of them have ability of nitrogen fixation and phosphate solubilization together with IAA biosynthesis. Population of nitrogen-fixing and phosphate-solubilizing bacteria correlated with N total concentration and organic matter content in soil closely (P<0.05). The sequences from selected nitrogen-fixing and phosphate-solubilizing bacteria (12 isolates) showed high degrees of similarity to those of the GenBank references strains (between 98% and 99%). From 12 isolates, 7 belonged to Bacilli, while 2 were Beta-Proteobacteria, 1 was Acidobacteria and 2 were Bacteroides. Based on Pi value (nucleotide diversity), Bacilli group had the highest theta value and Theta values (persequence) from S of SNP for DNA polymorphism were calculated for each group and Bacilli group had the highest values in comparison to three groups. From these results showed that two strains (Bacillus megaterium B6 (Ferrasols) and Sphingomonas sp. P14 (Acrisols)) revealed promising candidates with multiple beneficial characteristics and they have the potential for application as inoculants adapted to poor soils and sugarcane tree because they are not only famous strains but also are safety strains for agricultural sustainable.

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Characterization of Rhizobacteria from field grown Genetically Modified (GM) and non-GM maizes

Abstract: This study was done to examine the rhizobacteria from field grown Genetically Modified (GM)

Cited by 33 publications

References 36 publications

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

SERS detection of the biomarker hydrogen cyanide from Pseudomonas aeruginosa cultures isolated from cystic fibrosis patients

Peer Review #1 of "Preliminary study on phosphate solubilizing Bacillus subtilis strain Q3 and Paenibacillus sp. strain Q6 for improving cotton growth under alkaline conditions (v0.1)"

Isolation and Identification of Rhizospheric Bacteria in Sugarcane (Saccharum spp. L.) Cultivated on Acrisols and Ferrasols of Dong Nai Province, the Southeast of Vietnam

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