Road traffic signs are part of the road infrastructure components meant to ensure the safe use of roads by motorists and pedestrians alike. A good knowledge and comprehension of road signs helps ensure smooth flow of traffic, improves safety for other motorists and provides drivers with good reaction time to comply with the message dictated by the signs. Abu Dhabi, being the capital of the UAE, brings together people from all over the world for work, business or tourism. Abu Dhabi has quality roads and traffic signage together with an efficient police force that enforces adherence to traffic rules. Despite all these and the reduced fatalities in absolute terms, traffic violations have been increasing exponentially, resulting in five million traffic fines in 2019 for a population of about 2.9 million inhabitants. This study sought to assess motorists’ comprehension of the various traffic signs used across the UAE. The results of the survey (N=200) revealed that the drivers were able to correctly identify 77% of the road signs in the survey. It was also found that the respondents failed to correctly identify traffic signs within the specific category referred to as “advance warning signs”. Respondents’ education, nationality, gender, and marital status did not have any statistically significant effects on the results compared to earlier studies. It is recommended that more attention be focused on driver education and training.