Characterization of Silver Nanoparticles Synthesizing Bacteria and Its Possible Use in Treatment of Multi Drug Resistant Isolate

Abstract

Nanobiotechnology is apromisingarea to cater human life. Biological methods for the synthesis of silver nanoparticles are relatively cost effective process. It was aimed at synthesizing silver nanoparticles using water and soil borne bacterial isolates, characterization of silver nanoparticles using UV-Vis spectroscopy and Fourier transform infra red (FTIR) spectroscopy and its effect on multi drug resistant bacterial isolate. The biosynthesis of silver nanoparticles was evaluated in both bacterial biomass and culture supernatant of Bacillus, Pseudomonas and Escherichia coli in the presence of 1mM of AgNO 3 . On the basis of physical appearances of silver nano particles, Pseudomonas sp. was selected for synthesis. The absorbance spectra of reaction mixture of bacterial biomass and supernatant show the strong peak at 420 nm, indicating the presence of silver nanoparticles (AgNPs) using UV-Vis and FTIR spectrophotometry. The influence of synthesized AgNPs wastested against multi drug resistant (MDR) Staphylococcus sp. on Mueller Hinton agar. The multi antibiotics resistant Staphylococcus sp. Showe dantibiotic sensitivity against the antibiotic discs impregnated with silver nanoparticles. The characteristics of silver nanoparticlesrevealedits possible use in biomedical field.

BIOSYNTHESIS, CHARACTERIZATION, BACTERICIDAL AND TOXIC EFFECT OF SILVER NANOPARTICLES USING Klebsiella pneumoniae STRAIN STC1

“…A visual change of color from yellow to brown might be due to the excitation of surface plasmon resonance in silver nanoparticles. [3] This change in color was confirmed by other studies. [2,3,6,23] The surface plasmon vibrations were due to the dipole oscillation arising when an electromagnetic field in the visible range was coupled to the collective oscillations of conduction electrons.…”

“…[3] This change in color was confirmed by other studies. [2,3,6,23] The surface plasmon vibrations were due to the dipole oscillation arising when an electromagnetic field in the visible range was coupled to the collective oscillations of conduction electrons. [24] The light microscopic images showed that silver nanoparticles' aggregates were precipitated outside the bacterial cells indicating an extracellular production of silver nanoparticles (Fig.…”

“…In the recent past, silver nanoparticles had gained the attention of researchers worldwide due to their potential antimicrobial activities. [2,3] The antibacterial effect was influenced by blocking respiratory enzyme pathways, interacting with the sulfur-containing proteins, and modification of microbial DNA. In the present study, the synthesized nanoparticles were characterized by UV, FTIR, XRD, and SEM.…”

“…Similar to our findings, other researchers have also reported silver nanoparticles' formation by exhibiting the typical surface plasmon absorption maxima at nearly 412 nm from the UV-visible spectrum. [3,25] The characterization of the silver nanoparticles was also investigated by analyzing FTIR spectra. The spectral analysis of the experimental sample was shown in Fig.…”

Study the effect of Ag nanoparticles and ZnO nanoparticles at different concentrations on the viscosity and surface tension of Polyacrylamide solutions

“…Silver nanoparticles are introduced into bactericidal agents, It is considered a means of transporting antibiotics [3]. Nanoparticles were used as colloidal silver, it has been studied extensively in the field of biotechnology, it is nontoxic and safe in the treatment of microbial diseases [4].…”