Characterization of single-nucleotide polymorphisms relevant to inflammatory bowel disease in commonly used gastrointestinal cell lines

Hüebner, Claudia; Petermann, Ivonne; Lam, Wen Jiun; Shelling, Andrew N.; Ferguson, Lynnette R.

doi:10.1002/ibd.21043

Cited by 11 publications

(12 citation statements)

References 85 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Shed CL as well as the intestinal phospholipid barrier may represent another mechanism by which monophosphorylated family Bacteroidaceae-type LPS in the intestinal lumen due to significantly lower binding to LBP and transfer to CD14 (52) is neutralized before engagement of TLR4. In addition, both LBP and CD14 are made by intestinal epithelial cells (62,63), and evidence that they contribute to intestinal homeostasis has been reported (64,65). Furthermore, modulation of TLR4 activity by LPB may have some clinical utility in treating necrotizing enterocolitis (66).…”

Section: Discussionmentioning

confidence: 99%

Cardiolipins Act as a Selective Barrier to Toll-Like Receptor 4 Activation in the Intestine

Coats

Hashim

Paramonov

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

Intestinal homeostasis mechanisms must protect the host intestinal tissue from endogenous lipopolysaccharides (LPSs) produced by the intestinal microbiota. In this report, we demonstrate that murine intestinal fecal lipids effectively block Toll-like receptor 4 (TLR4) responses to naturally occurring Bacteroidetes sp. LPS. Cardiolipin (CL) represents a significant proportion of the total intestinal and fecal lipids and, furthermore, potently antagonizes TLR4 activation by reducing LPS binding at the lipopolysaccharide binding protein (LBP), CD14, and MD-2 steps of the TLR4 signaling pathway. It is further demonstrated that intestinal lipids and CL are less effective at neutralizing more potent Enterobacteriaceae-type LPS, which is enriched in feces obtained from mice with dextran sodium sulfate (DSS)-treated inflammatory bowel disease. The selective inhibition of naturally occurring LPS structures by intestinal lipids may represent a novel homeostasis mechanism that blocks LPS activation in response to symbiotic but not dysbiotic microbial communities. IMPORTANCEThe guts of animals harbor a variety of Gram-negative bacteria associated with both states of intestinal health and states of disease. Environmental factors, such as dietary habits, can drive the microbial composition of the host animal's intestinal bacterial community toward a more pathogenic state. Both beneficial and harmful Gram-negative bacteria are capable of eliciting potentially damaging inflammatory responses from the host intestinal tissues via a lipopolysaccharide (LPS)-dependent pathway. Physical mucosal barriers and antibodies produced by the intestinal immune system protect against the undesired inflammatory effects of LPS, although it is unknown why some bacteria are more effective at overcoming the protective barriers than others. This report describes the discovery of a lipid-type protective barrier in the intestine that reduces the deleterious effects of LPSs from beneficial bacteria but is less effective in dampening the inflammatory effects of LPSs from harmful bacteria, providing a novel mechanistic insight into inflammatory intestinal disorders.T he intestinal tract is constantly exposed to the potentially harmful effects of lipopolysaccharide (LPS), commonly referred to as endotoxin. Both internal sources, such as the intestinal microbiome, and external sources, such as food, provide a constant potential for endotoxin-mediated local or systemic injury. Several different innate and adaptive protective mechanisms present in the intestine facilitate a homeostatic relationship with the resident commensal microbiota and buffer food intake such that LPS does not impair normal intestinal tissue functions (1-5).Studies have shown that a major mechanism by which the intestine limits exposure to the potentially harmful effects of LPS is by preventing intestinal LPS-epithelial cell interactions through a physiochemical barrier consisting of a family of glycoproteins referred to as mucins. Mucins provide an effective barricade restrict...

show abstract

Section: Discussionmentioning

confidence: 99%

Cardiolipins Act as a Selective Barrier to Toll-Like Receptor 4 Activation in the Intestine

Coats

Hashim

Paramonov

et al. 2016

Appl Environ Microbiol

View full text Add to dashboard Cite

show abstract

“…SNPs can have an impact on gene expression as well as the biological function of protein, which leads to phenotypic consequences [57]. SNPs in the LPB gene are associated with susceptibility to sepsis and multiple organ dysfunction [58], [59].…”

Section: Discussionmentioning

confidence: 99%

Ovocalyxin-36 Is a Pattern Recognition Protein in Chicken Eggshell Membranes

et al. 2013

View full text Add to dashboard Cite

The avian eggshell membranes are essential elements in the fabrication of the calcified shell as a defense against bacterial penetration. Ovocalyxin-36 (OCX-36) is an abundant avian eggshell membrane protein, which shares protein sequence homology to bactericidal permeability-increasing protein (BPI), lipopolysaccharide-binding protein (LBP) and palate, lung and nasal epithelium clone (PLUNC) proteins. We have developed an efficient method to extract OCX-36 from chicken eggshell membranes for purification with cation and anion exchange chromatographies. Purified OCX-36 protein exhibited lipopolysaccharide (LPS) binding activity and bound lipopolysaccharide (LPS) from Escherichia coli O111:B4 in a dose-dependent manner. OCX-36 showed inhibitory activity against growth of Staphylococcus aureus ATCC 6538. OCX-36 single nucleotide polymorphisms (SNPs) were verified at cDNA 211 position and the corresponding proteins proline-71 (Pro-71) or serine-71 (Ser-71) were purified from eggs collected from genotyped hens. A significant difference between Pro-71 and Ser-71 OCX-36 for S. aureus lipoteichoic acid (LTA) binding activity was detected. The current study is a starting point to understand the innate immune role that OCX-36 may play in protection against bacterial invasion of both embryonated eggs (relevant to avian reproductive success) and unfertilized table eggs (relevant to food safety).

show abstract

“…The Caco-2 cell line and bacterial strains were cultured as described previously (4,11). Monolayers were seeded in 24-well tissue culture plates with 2 ϫ 10 5 cells/well and incubated for 14 days.…”

mentioning

confidence: 99%

“…Reverse transcription of 1.4 g of total RNA to cDNA was carried out using the SuperScript III first-strand synthesis super mix kit (Invitrogen, Auckland, New Zealand) and oligo(dT) 20 primers according to the standard protocol. Reactions were performed in 384-well plates as previously described (11), and the cDNA was amplified in an Applied Biosystems ABI PRISM 7900HT sequence detection system. The primers used for quantitative PCR (qPCR) are shown in Table 2.…”

mentioning

confidence: 99%

The Probiotic Escherichia coli Nissle 1917 Reduces Pathogen Invasion and Modulates Cytokine Expression in Caco-2 Cells Infected with Crohn's Disease-Associated E. coli LF82

Hüebner

Ding

Petermann

et al. 2011

Appl Environ Microbiol

Self Cite

View full text Add to dashboard Cite

show abstract

Characterization of single-nucleotide polymorphisms relevant to inflammatory bowel disease in commonly used gastrointestinal cell lines

Cited by 11 publications

References 85 publications

Cardiolipins Act as a Selective Barrier to Toll-Like Receptor 4 Activation in the Intestine

Cardiolipins Act as a Selective Barrier to Toll-Like Receptor 4 Activation in the Intestine

Ovocalyxin-36 Is a Pattern Recognition Protein in Chicken Eggshell Membranes

The Probiotic Escherichia coli Nissle 1917 Reduces Pathogen Invasion and Modulates Cytokine Expression in Caco-2 Cells Infected with Crohn's Disease-Associated E. coli LF82

Contact Info

Product

Resources

About