Characterization of the complete mitochondrial genome of the New Zealand parasitic blowflyCalliphora vicina(Insecta: Diptera: Calliphoridae)

Abstract

In the present study, the complete mitochondrial genome of the New Zealand parasitic blowfly Calliphora vicina (blue bottle blowfly) field strain NZ_CalVic_NP was generated using next-generation sequencing technology and annotated. The 16,518 bp mitochondrial genome consists of 13 protein-coding genes, two ribosomal RNAs, 22 transfer RNAs, and a 1,689 bp non-coding region, similar to most metazoan mitochondrial genomes. Phylogenetic analysis showed that C. vicina NZ_CalVic_NP does not form a monophyletic cluster with the remaining three Calliphorinae species. The complete mitochondrial genome sequence of C. vicina NZ_CalVic_NP is a resource to facilitate future species identification research within the Calliphoridae.

16S rRNA Gene Amplicon Profiling of the New Zealand parasitic blowflyCalliphora vicina

“…Currently control relies heavily on the prophylactic application of long-acting chemicals to all sheep, but this approach is increasingly under threat due to development of resistance to current treatments (2, 3). Calliphora vicina NZ_CalVic_NP (4, 5) was selected for microbiome assessment as a representative of a New Zealand field strain of C. vicina . In this study, we have investigated the larval, adult male and adult female bacterial microbial profiles of C. vicina to gain a better understanding of the microbial communities of blowflies targeting the development of new interventions such as probiotics, bioactive compounds, vaccines or insecticides.…”