Characterization of the Cryptic AV3 Promoter of Ageratum Yellow Vein Virus in Prokaryotic and Eukaryotic Systems

WeiChen, Wang; Wu, Chia‐Ying; Lai, Yi-Chin; Lin, Na-Sheng; Hsu, Yau-Heiu; Hu, Chung-Chi

doi:10.1371/journal.pone.0108608

Cited by 4 publications

(1 citation statement)

References 54 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Differing putative promoter, cis -acting element, and iteron sequences present in this region could also be exerting an effect. Although promoter elements have not yet been mapped for ToCSV, sequences with promoter activity have been found within coding regions of other begomoviruses [ 62 , 85 , 86 ]. Promoter mapping of the two variant sequences may uncover additional sequence features which are implicated in differentiating pathogenicity through their varied activities.…”

Section: Discussionmentioning

confidence: 99%

Differences in the 3’ intergenic region and the V2 protein of two sequence variants of tomato curly stunt virus play an important role in disease pathology in Nicotiana benthamiana

Zwolinski,

Brigden,

Rey

2023

PLoS ONE

View full text Add to dashboard Cite

Tomato production in South Africa is threatened by the emergence of tomato curly stunt virus (ToCSV), a monopartite Begomovirus transmitted by the whitefly vector Bemisia tabaci (Genn.). We investigated the role of sequence differences present in the 3’ intergenic region (IR) and the V2 coding region on the differing infectivity of ToCSV sequence variant isolates V30 and V22 in the model host Nicotiana benthamiana. Using virus mutant chimeras, we determined that the development of the upward leaf roll symptom phenotype is mediated by sequence differences present in the 3’ IR containing the TATA-associated composite element. Sequence differences present in the V2 coding region are responsible for modulating disease severity and symptom recovery in V22-infected plants. Serine substitution of V22 V2 Val27 resulted in a significant increase in disease severity with reduced recovery, the first study to demonstrate the importance of this V2 residue in disease development. Two putative ORFs, C5 and C6, were identified using in silico analysis and detection of an RNA transcript spanning their coding region suggests that these ORFs may be transcribed during infection. Additional virus-derived RNA transcripts spanning multiple ORFs and crossing the boundaries of recognised polycistronic transcripts, as well as the origin of replication within the IR, were detected in ToCSV-infected plants providing evidence of bidirectional readthrough transcription. From our results, we conclude that the diverse responses of the model host to ToCSV infection is influenced by select sequence differences and our findings provide several avenues for further investigation into the mechanisms behind these responses to infection.

show abstract

Section: Discussionmentioning

confidence: 99%