Characterization of the expression profile of calpain-3 (CAPN3) gene in chicken

Abstract

Calpain-3 is a skeletal muscle-specific protease and participates in the regulation of myogenesis. In this study, we quantified the expression of calpain-3 (CAPN3) mRNA in a Chinese local chicken breed (Sichuan Mountainous Black-boned chicken [MB]), to discern the tissue and ontogenic expression pattern. Meanwhile, we compared the CAPN3 mRNA expression pattern in MB chicken at 10 weeks with a commercial meat type chicken line (S01) of the same age to identify the unique expression pattern under different genetic background. A real time quantitative PCR (qRT-PCR) assay was developed for an accurate measurement of its expression in various tissues from chickens at different ages (0, 2, 4, 6, 8, 10, and 12 weeks). Expression of the CAPN3 mRNA was detected in the selected tissues, regardless of age. The breast muscle and leg muscle tissues had a significantly higher expression than the other tissues from the same individual (P < 0.01). Overall, the CAPN3 mRNA level exhibited a "rise-decline" developmental change in detected tissues except for brain. The S01 chicken had a higher expression of the CAPN3 mRNA in detected tissues than the MB chicken at 10 weeks. The present expression data of chicken CAPN3 gene may provide some information to shed light on the tissue and ontogenic expression pattern during chicken development.

The normalisation ofCAPNgene expression inM. pectoralis superficialisin broiler lines differing in growth rate and their relationship to breast muscle tenderness

“…However, in the present study, significant results were observed only for the CAPN1 gene; for the CAPN3 gene expression, only a trend was observed. For the CAPN3 gene, similar results were obtained by Zhang et al (2012), although the authors assessed CAPN3 mRNA abundance at 10 weeks of age for both slow-(the Sichuan Mountainous Black-boned chicken) and fastgrowing lines (line S01). In this study, birds characterised by high shear force (low tenderness) showed a lower level of CAPN3 gene expression than broilers with low shear force, which is consistent with the trend obtained in our study.…”

Characterization of the Goose <i>CAPN3</i> Gene and its Expression Pattern in Muscle Tissues of Sichuan White Geese at Different Growth Stages

“…Our results showed that CAPN3 was expressed in both muscle tissues, which is consistent with a report that the level of CAPN3 gene expression in the breast muscle and leg muscle was significantly higher than that in other tissues in chickens (Zhang et al, 2012). Wu et al (2015) reported high levels of CAPN3 expression in the muscle atrophic phase after denervation.…”

“…This result is consistent with the zoological classification and suggests that CAPN3 protein is relatively evolutionarily conserved. Meanwhile, consistent with CAPN3 protein in other animals, such as cattle and chickens (Zhang et al, 2012;Pan et al, 2013;Wang et al, 2016), the Sichuan white goose CAPN3 protein has no signal peptide.…”

“…In mammals, CAPN3 is the most highly expressed in skeletal muscles, especially in the fast (type II) fibers (Jones et al, 1999), but CAPN3 mRNA has also been detected in the heart (Fougerousse et al, 2000). In chickens, Zhang et al (2012) and Sorimachi et al (1995) reported that CAPN3 is abundantly expressed in the skeletal muscle and is also expressed in the liver, heart, and brain. Subsequent studies by Ilian et al (2001Ilian et al ( , 2004 and Felício et al (2013) revealed significant correlations between meat quality and the expression or polymorphism of CAPN3.…”

Identification and association of polymorphisms in CAPN1 and CAPN3 candidate genes related to performance and meat quality traits in chickens

“…Zhang et al (2009) identified 2 SNPs (11818T>A and 12814T>G) in the CAPN3 gene in outbred strains of chicken and found associations with body weight, carcass weight, breast muscle weight, and leg muscle weight. In chickens, the CAPN3 gene was highly expressed in muscle tissues of breast and legs at ages 0, 2, 4, 6, 8, 10, and 12 months (Zhang et al, 2012).…”