Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs)

Zanaroli, Giulio; Balloi, Annalisa; Negroni, Andrea; Daffonchio, Daniele; Young, L. Y.; Fava, Fabio

doi:10.1016/j.jhazmat.2010.01.097

Cited by 47 publications

(32 citation statements)

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“…2.5 ± 0.2 mmol) of methane were produced (Additional file 3: Figure S2). Therefore, methane production was not related to the dechlorination activities detected, as previously observed in the same area [35, 42]. …”

Section: Resultssupporting

confidence: 55%

“…In sediments of the Gulf of Taranto (Mar Piccolo, Ionian Sea, Italy), the enrichment of Dehalococcoides mccartyi and its PCB reductive dehalogenase-coding genes was reported during PCB dechlorination [40, 41]. Conversely, non- Dehalococcoides Dehalococcoidia were detected in sediments of the Venice Lagoon after sub-culturing of the indigenous community in the presence of exogenous PCBs [34, 42] as well as after biostimulation with zerovalent iron nanoparticles [43], suggesting that this group of organohalide respiring Chloroflexi might be relevant for the bioremediation of PCB-contaminated marine sediments. The aims of this work were: (i) to further assess the dechlorination potential, in terms of occurrence, dechlorination rate and specificity, of the indigenous microbial communities of the PCB-contaminated sediments in the Porto Marghera area (Venice Lagoon) and (ii) to assess the role of Dehalococcoidia in the process in terms of dehalogenation specificity and growth during organohalide respiration.…”

Section: Introductionmentioning

confidence: 99%

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Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

et al. 2017

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BackgroundMicrobial reductive dechlorination of polychlorinated biphenyls (PCBs) plays a major role in detoxifying anoxic contaminated freshwater and marine sediments from PCBs. Known members of the phylum Chloroflexi are typically responsible for this activity in freshwater sediments, whereas less is known about the microorganisms responsible for this activity in marine sediments. PCB-respiring activities were detected in PCB-impacted marine sediments of the Venice Lagoon. The aim of this work was to identify the indigenous organohalide-respiring microorganisms in such environments and assess their dechlorination specificity against spiked Aroclor™ 1254 PCBs under laboratory conditions resembling the in situ biogeochemistry.ResultsHigh PCB dechlorination activities (from 150 ± 7 to 380 ± 44 μmol of chlorine removed kg−1 week−1) were detected in three out of six sediments sampled from different locations of the lagoon. An uncultured non-Dehalococcoides phylotype of the class Dehalococcoidia closely related to Dehalobium chlorocoercia DF-1, namely phylotype VLD-1, was detected and enriched up to 109 16S rRNA gene copies per gram of sediment where dechlorination activities were higher and 25-4/24-4 and 25-2/24-2/4-4 chlorobiphenyls (CB) accumulated as the main tri-/dichlorinated products. Conversely, a different phylotype closely related to the SF1/m-1 clade, namely VLD-2, also enriched highly where lower dechlorination activity and the accumulation of 25-3 CB as main tri-chlorinated product occurred, albeit in the simultaneous presence of VLD-1. Both phylotypes showed growth yields higher or comparable to known organohalide respirers and neither phylotypes enriched in sediment cultures not exhibiting dechlorination.ConclusionsThese findings confirm the presence of different PCB-respiring microorganisms in the indigenous microbial communities of Venice Lagoon sediments and relate two non-Dehalococcoides phylotypes of the class Dehalococcoidia to different PCB dechlorination rates and specificities.Electronic supplementary materialThe online version of this article (doi:10.1186/s12934-017-0743-4) contains supplementary material, which is available to authorized users.

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Section: Resultssupporting

confidence: 55%

Section: Introductionmentioning

confidence: 99%

Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

et al. 2017

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“…The ratio between solid material and the aqueous phase was strongly decreased. Cultivation was performed in 600 ml of liquid medium, which was 10 to 60 times the volume reported in the literature (Wu et al, 1998;Watts et al, 2005;Bedard et al, 2007;D'Angelo and Nunez, 2010;Zanaroli et al, 2010). This volume allowed for multiple samplings of total DNA and RNA and could equilibrate the possible impact of the elimination of chloride ions during the OHR process, as pH has been shown to be of critical importance (Lacroix et al, 2012).…”

Section: Discussionmentioning

confidence: 99%

Divergent PCB organohalide-respiring consortia enriched from the efflux channel of a former Delor manufacturer in Eastern Europe

Praveckova

Brennerova

Čvančarová

et al. 2015

Ecotoxicology and Environmental Safety

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“…The dechlorination of pentachlorophenols was detected in the sludge from a pulp and paper mill (Karn et al, 2011). Reductive dechlorination of CPs, biphenyls, and benzenes has been reported in a number of sedimentary environments (Wu et al, 1998;Fagervold et al, 2005;Zanaroli et al, 2010;Kjellerup et al, 2014;Kuokkaa et al, 2014;Vandermeeren et al, 2014).…”

Section: Introductionmentioning

confidence: 98%

Characterization of a Highly Enriched Microbial Consortium Reductively Dechlorinating 2,3-Dichlorophenol and 2,4,6-Trichlorophenol and the Corresponding cprA Genes from River Sediment

El‐Sayed

2016

Polish Journal of Microbiology

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A b s t r a c tAnaerobic reductive dechlorination of 2,3-dichlorophenol (2,3DCP) and 2,4,6-trichlorophenol (2,4,6TCP) was investigated in microcosms from River Nile sediment. A stable sediment-free anaerobic microbial consortium reductively dechlorinating 2,3DCP and 2,4,6TCP was established. Defined sediment-free cultures showing stable dechlorination were restricted to ortho chlorine when enriched with hydrogen as the electron donor, acetate as the carbon source, and either 2,3-DCP or 2,4,6-TCP as electron acceptors. When acetate, formate, or pyruvate were used as electron donors, dechlorination activity was lost. Only lactate can replace dihydrogen as an electron donor. However, the dechlorination potential was decreased after successive transfers. To reveal chlororespiring species, the microbial community structure of chlorophenol-reductive dechlorinating enrichment cultures was analyzed by PCR-denaturing gradient gel electrophoresis (DGGE) of 16S rRNA gene fragments. Eight dominant bacteria were detected in the dechlorinating microcosms including members of the genera Citrobacter, Geobacter, Pseudomonas, Desulfitobacterium, Desulfovibrio and Clostridium. Highly enriched dechlorinating cultures were dominated by four bacterial species belonging to the genera Pseudomonas, Desulfitobacterium, and Clostridium. Desulfitobacterium represented the major fraction in DGGE profiles indicating its importance in dechlorination activity, which was further confirmed by its absence resulting in complete loss of dechlorination. Reductive dechlorination was confirmed by the stoichiometric dechlorination of 2,3DCP and 2,4,6TCP to metabolites with less chloride groups and by the detection of chlorophenol RD cprA gene fragments in dechlorinating cultures. PCR amplified cprA gene fragments were cloned and sequenced and found to cluster with the cprA/pceA type genes of Dehalobacter restrictus.

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Characterization of the microbial community from the marine sediment of the Venice lagoon capable of reductive dechlorination of coplanar polychlorinated biphenyls (PCBs)

Cited by 47 publications

References 33 publications

Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

Identification of two organohalide-respiring Dehalococcoidia associated to different dechlorination activities in PCB-impacted marine sediments

Divergent PCB organohalide-respiring consortia enriched from the efflux channel of a former Delor manufacturer in Eastern Europe

Characterization of a Highly Enriched Microbial Consortium Reductively Dechlorinating 2,3-Dichlorophenol and 2,4,6-Trichlorophenol and the Corresponding cprA Genes from River Sediment

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