Characterization of the Nucleotide Sequence of a Polyubiquitin Gene (PUBC1) from Arabian Camel, Camelus dromedarius

Abstract

Molecular amplification and sequencing of genomic DNA that encodes camel polyubiquitin (PUBC1) was performed by a polymerase chain reaction (PCR) using various sets of primers. The amplification generated a number of DNA fragments, which were sequenced and compared with the polyubiquitin coding sequences of various species. One DNA fragment that conformed to 325 bp was found to be 95 and 88% homologous to the sequences of human polyubiquitin B and C, respectively. The DNA translated into 108 amino acids that corresponded to two fused units of ubiquitin with no intervening sequence, which indicates that it is a polyubiquitin and contains at least two units of ubiquitin. Although, variations were found in the nucleotide sequence when compared to those of other species, the amino acid sequence was 100% homologous to the polyubiquitin sequences of humans, mice, and rats. This is the first report of the polyubiquitin DNA coding sequence and its corresponding amino acid sequence from camels, amplified using direct genomic DNA preparations.

Identification of COVID‐19 Infection‐Related Human Genes Based on a Random Walk Model in a Virus–Human Protein Interaction Network

“…The final discussed protein is UBBP4 ( ENSP00000464265 ), which has been widely reported to act as a pseudogene and is functionally correlated with psoriasis [ 51 , 52 ]. Similar to UBL4A and UBL4B, UBBP4 can contribute to the regulation of the ERAD pathway [ 53 ] and therefore may be functionally correlated with COVID-19 infection. This relationship validates the efficacy and accuracy of our prediction.…”

Molecular Diversity and Phylogenetic Analysis of Eight Dromedary Camel Breeds of Pakistan Based on Mitochondrial Atp6 and Atp8 Genes

“…Another South American Camelid study, which validated the use of control region of mitochondrial genome as a molecular biomarker to deduce data on Camelid genetic relationships and population biodiversity (Mate et al, 2004). Similarly, nucleotide sequence of a polyubiquitin gene (PUBC1) of (Camelus dromedarius) was also used to have an insight of genetic diversity in one of the Arabian camel study, which revealed that there are genetic variation, one of the 325 bp motif was 95 and 88% identical to human polyubiquitin genes B and C respectively and synonymous mutations were observed, while their amino acid sequence was 100% homologous (Al-Khedhairy, 2004). Microsatellite markers can also be served as a tool for determining population diversity, one of the Egyptian studies revealed that RAPD markers results showed genetic variation between and within different camel breeds.…”

Molecular cloning and cDNA characterization of Camelus dromedarius putative cytochrome P450s 1A1, 2C, and 3A

“…This camel is found in the Arabian deserts and arid and semiarid areas of the Middle East (Thomas et al, 1985;Hussein et al, 1992;Al-Khedhairy, 2004). The Arabian camel has unique physiological modifications and is able to live in harsh desert conditions, such as high temperature, direct exposure to sunlight and lack of water resources, with this animal being able to survive without drinking water for weeks (Hussein et al, 1992).…”

“…The DNA sequence of camels exhibits wide variations with other species, including human; however, functional similarities exist. Therefore, studies of camel protein represent a potentially viable target for human applications (Al-Khedhairy, 2004).…”