Characterization of the nucleotide sequence of the Lymantria dispar nuclear polyhedrosis virus DNA polymerase gene region

Bjornson, Rebecca M.; Glocker, B; Rohrmann, George F.

doi:10.1099/0022-1317-73-12-3177

Cited by 31 publications

(22 citation statements)

References 25 publications

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“…Recently, the complete nucleotide sequence of the AcMNPV has been reported (Ayres et al, 1994). Although several genes that are present in both LdMNPV and AcMNPV have been identified [polyhedrin (Smith et al, 1988), p39 (Bjornson & Rohrmann, 1992b), vPK (Bischoff & Slavicek, 1994), egt (Riegel et aL, 1994) and D N A polymerase (Bjornson et al, 1992)], there is no G22 gene homologue present in AcMNPV.…”

Section: T a T T A T A A C T T T T A T T T C C T C A T T G T A A A A mentioning

confidence: 99%

“…A number of LdMNPV genes that are also present in D. S. Bischoff and J. M. Slavicek AcMNPV have been identified and characterized, including ecdysteroid UDP-glucosyl transferase (Riegel et al, 1994), DNA polymerase (Bjornson et al, 1992), polyhedral envelope (Bjornson & Rohrmann, 1992a), polyhedrin (Smith et al, 1988), p39 nucleocapsid (Bjornson & Rohrmann, 1992b) and viral protein kinase (Bischoff & Slavicek, 1994).…”

Section: Fswa/s-jslavicek/ou-s24lo5a@mhsattmailcommentioning

confidence: 99%

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Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Bischoff

Slavicek

1995

Journal of General Virology

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The Lymantria dispar multinucleocapsid nuclear polyhedrosis virus (LdMNPV) gene encoding G22 was cloned and sequenced. The G22 gene codes for a 191 amino acid protein with a predicted M r of 22000. Expression of G22 in a rabbit reticulocyte system generated a protein with an M r of 24000, in close agreement with the molecular mass predicted from the nucleotide sequence. G22 is not significantly homologous to any known protein, nor is a G22 homologue present in the Autographa californica MNPV (AcMNPV). Temporal expression studies indicated that the G22 gene was transcribed at readily detectable levels in the presence of cycloheximide. Transcripts were detected immediately after the virus adsorption period and throughout the infection cycle. The early transcriptional start sites of G22 map to a sequence that resembles a subset of RNA polymerase II promoters/start sites that are found upstream of Drosophila melanogaster developmental and retrotransposon genes which lack TATA box motifs. Several consensus late baculovirus promoter/ mRNA start site sequences (ATAAG) were identified upstream of the G22 gene start codon.

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Section: T a T T A T A A C T T T T A T T T C C T C A T T G T A A A A mentioning

confidence: 99%

Section: Fswa/s-jslavicek/ou-s24lo5a@mhsattmailcommentioning

confidence: 99%

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Bischoff

Slavicek

1995

Journal of General Virology

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“…The 20 kbp of overlapping D N A contained within C12 and C77 was subcloned and the mutation further mapped in marker LdMNPV genes are indicated: polh (polyhedrin; Smith et al, 1988), EGT (ecdysteriod UDPglucosyltransferase; Riegel et al, 1994), DNA pol (DNA polymerase; Bjornson et al, 1992) and PK (viral protein kinase; Bischoff & Slavicek, 1994 ) TATATTAAAATAGTTTGTG TATTAAA TAGTTTG TATATTAAAATAGTTTG TATATTAAA TAGTTTG TATATTAAA TAGTTTGTG TATTAAA TAGTTTGTG TATTAA TAGTTTGTA TATTAAAATAGTTTGAC TGTTAAAATAGTT GAGC TATTAAAATAGTTTGGC TATTAGAATAGTTTG TATTAAA TTGTTTA TACATTAAAA TTA TAT GTC ATC GTC GGA ATT TGT …”

Section: Marker Rescue Of T H E a 2 1 -2 Mutationmentioning

confidence: 99%

Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene

Bischoff

Slavicek

1996

Journal of General Virology

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“…The viral genome has been mapped with restriction endonucleases (Smith et al, 1988;Riegel et al, 1994), and transcription and translation maps have been generated (Slavicek, 1991). A number of genes that have been previously identified in the AcMNPV including the ecdysteroid UDP-glucosyl transferase (Riegel et al, 1994), DNA polymerase (Bjornson et al, 1992), polyhedral envelope protein (Bjornson and Rohrmann, 1992a), polyhedrin (Smith et al, 1988), p39 nucleocapsid (Bjornson and Rohrmann, 1992b), and viral protein kinase (Bischoff and Slavicek, 1994), have been located and characterized in the LdMNPV. Even though several LdMNPV genes have been characterized at the structural level the life cycle of this virus had not been detailed.…”

Section: Introductionmentioning

confidence: 99%

Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus

Riegel

Slavicek

1997

Virus Research

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The life cycle of the Lymantria dispar nuclear polyhedrosis virus (LdMNPV) was characterized through analysis of budded virus (BV) release, the temporal formation of polyhedra, the temporal transcription pattern of representative early, late, and hyper-expressed late genes, and the onset of DNA replication in the Ld652Y cell line. Transcripts from the LdMNPV immediate early gene G22 were detected 4 h post infection (h p.i.). The late and hyper-expressed late p39 capsid and polyhedrin genes were initially transcribed at approximately 20 and 24 h p.i., respectively. Viral DNA replication initiated at approximately 18-20 h p.i. Budded virus was released from infected cells between 24 and 36 h p.i., and polyhedra were first detected at approximately 48 h p.i. © 1997 Elsevier Science B.V.

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Characterization of the nucleotide sequence of the Lymantria dispar nuclear polyhedrosis virus DNA polymerase gene region

Cited by 31 publications

References 25 publications

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Identification and characterization of an early gene in the Lymantria dispar multinucleocapsid nuclear polyhedrosis virus

Characterization of the Lymantria dispar nucleopolyhedrovirus 25K FP gene

Characterization of the replication cycle of the Lymantria dispar nuclear polyhedrosis virus

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