Characterization of the PEG layer of sterically stabilized liposomes: a SAXS study

Varga, Zoltán; Wacha, András; Vainio, Ulla; Gummel, Jérémie; Bóta, Attila

doi:10.1016/j.chemphyslip.2011.12.011

Cited by 34 publications

(30 citation statements)

References 35 publications

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“…Based on small angle X-ray scattering study, the thickness of the PEG 2000 layer on liposomes was estimated to be ~5 nm when the PEG 2000 fraction is 10 mol%. 34 The self-assembly of 2 gave a broad peak with d-spacing of 5.5 nm as measured by the present powder XRD analysis. The difference in thickness of each PEG moiety was attributable to difference in density of PEGylation and morphology, which may cause deformation of random coils of PEG.…”

mentioning

confidence: 92%

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes

Ding¹,

Minamikawa²,

Kameta³

et al. 2014

IJN

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Application of organic nanotubes (ONTs) into drug nanocarriers ultimately requires validation in live animals. For improving the dispersibility in biological media and in vivo distribution, the outer surface of an ONT was functionalized with polyethylene glycol (PEG) via the coassembly of an ONT-forming lipid with 5–20 mol% of a PEG-tethered lipid analogue (PEG-lipid). Firstly, the effect of PEGylation on the psysicochemical properties of ONTs, such as morphology and dispersibility, was investigated. PEGylation of ONTs slightly reduced the average length and effectively prevented the aggregation in phosphate-buffered saline (PBS). The PEGylated ONTs even showed high thermal stability in aqueous dispersion at least up to 95°C. Secondly, differential scanning calorimetry and powder X-ray diffraction indicated that ~10 mol% of PEG-lipid was completely incorporated into the ONTs, while 20 mol% of PEG-lipid encountered a partial phase separation during coassembly. In the heating differential scanning calorimetry runs, the resultant PEGylated ONTs with 5 mol% PEG-lipid showed no sign of phase separation up to 180°C under lyophilized condition, while those with 10 mol% and 20 mol% PEG-lipid showed some phase separation of the PEG-lipid above 120°C. Finally, PEGylation significantly affected the tissue distribution and prolonged the persistence time in the blood in mice. Non-PEGylated ONTs was quickly cleared from the circulation after intravenous infusion and preferentially accumulated in the lung, while PEGylated ONTs was mainly trapped in the liver and could circulate in the blood up to 24 hours. This study provided valuable information of physicochemical properties and the in vivo distribution behavior of PEGylated ONTs for their potential application into drug nanocarriers.

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mentioning

confidence: 92%

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes

Ding¹,

Minamikawa²,

Kameta³

et al. 2014

IJN

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“…Thus, they developed a moderated PEGylated (size 2 K) multicomponent liposome that hindered protein adsorption but still presented high cellular uptake in cancer cells [74]. Therefore, all of these studies were relevant for the development of efficient biohybrid liposomal pharmaceutical forms, contributing to the elucidation of their performance in the bloodstream [75,76].…”

Section: Liposomesmentioning

confidence: 99%

Advances in Hybrid Polymer-Based Materials for Sustained Drug Release

Ribeiro

Alcántara

Silva

et al. 2017

International Journal of Polymer Science

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The use of biomaterials composed of organic pristine components has been successfully described in several purposes, such as tissue engineering and drug delivery. Drug delivery systems (DDS) have shown several advantages over traditional drug therapy, such as greater therapeutic efficacy, prolonged delivery profile, and reduced drug toxicity, as evidenced by in vitro and in vivo studies as well as clinical trials. Despite that, there is no perfect delivery carrier, and issues such as undesirable viscosity and physicochemical stability or inability to efficiently encapsulate hydrophilic/hydrophobic molecules still persist, limiting DDS applications. To overcome that, biohybrid systems, originating from the synergistic assembly of polymers and other organic materials such as proteins and lipids, have recently been described, yielding molecularly planned biohybrid systems that are able to optimize structures to easily interact with the targets. This work revised the biohybrid DDS clarifying their advantages, limitations, and future perspectives in an attempt to contribute to further research of innovative and safe biohybrid polymer-based system as biomaterials for the sustained release of active molecules.

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“…Numerous attempts have been made to graft PEG in the bilayer surface for investigating the structure and nature of the bilayer membrane. However, most of the studies so far are limited to the nanometer scale [2,3] because of the instability of liposome bilayer in macroscale.…”

Section: Introductionmentioning

confidence: 99%

Lamellar–micelle transition in a hydrogel induced by polyethylene glycol grafting

2013

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We demonstrate, for the first time, a distinct change of the bulk properties of a hydrogel based on the lamellar-micelle transition. The hydrogel consists of thousands of periodical stacking of bilayer membranes, poly(dodecyl glyceryl itaconate) (PDGI), inside a dilute polyacrylamide network. Along with a bright structure color, the hydrogel exhibits strong anisotropy in its bulk properties. By grafting short chain polymer lipid, poly(ethylene glycol) dodecyl ether (C 12 EO 23 ), into the bilayer membrane, sharp changes in the structure color, swelling, and modulus of the hydrogel are observed in a narrow C 12 EO 23 concentration range.

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Characterization of the PEG layer of sterically stabilized liposomes: a SAXS study

Cited by 34 publications

References 35 publications

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes

Advances in Hybrid Polymer-Based Materials for Sustained Drug Release

Lamellar–micelle transition in a hydrogel induced by polyethylene glycol grafting

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Characterization of the PEG layer of sterically stabilized liposomes: a SAXS study

Cited by 34 publications

References 35 publications

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic&nbsp;nanotubes

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic&nbsp;nanotubes

Advances in Hybrid Polymer-Based Materials for Sustained Drug Release

Lamellar–micelle transition in a hydrogel induced by polyethylene glycol grafting

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Product

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Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes

Effects of PEGylation on the physicochemical properties and in vivo distribution of organic nanotubes