Characterization of the Peroxidase System in Winter Rye Seedlings:Compartmentation and Dependence on Leaf Development and Hydrogen Donors Used

Ievinsh, Gederts

doi:10.1016/s0176-1617(11)81076-x

Cited by 6 publications

(2 citation statements)

References 20 publications

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“…As described by Washko et al (1992), ascorbic acid oxidation in biological samples can be affected by numerous abiotic as well as enzymatic factors. Enzymes that directly or indirectly use ASC, such as ASC oxidase (Esaka et al, 1989) and peroxidases (Takahama and Oniki, 1992), are localized in the plant apoplast and are sometimes strongly bound to the cell wall fraction (Esaka et al, 1989;Ievinsh, 1992). Since it is possible that part of the cell wall fraction remains attached to the plasma membrane surface, it is also conceivable that some of these enzyme activities are still present in highly purified membrane preparations.…”

Section: Discussionmentioning

confidence: 99%

The Ascorbate Carrier of Higher Plant Plasma Membranes Preferentially Translocates the Fully Oxidized (Dehydroascorbate) Molecule

1997

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Recently, the uptake of 14C-labeled ascorbate (ASC) into highly purified bean (Phaseolus vulgaris 1.) plasma membrane vesicles was demonstrated in our laboratory. However, the question of the redox status of the transported molecule (ASC or dehydroascorbate [DHA]) remained unanswered. I n this paper we present evidence that DHA i s transported through the plasma membrane. High-performance liquid chromatography analysis of the redox status of ASC demonstrated that freshly purified plasma membranes exhibit a high ASC oxidation activity. Although it is not yet clear whether this activity is enzymatic, it complicates the interpretation of ASC-transport experiments in vitro and in vivo. In an attempt to correlate the ASC redox status to transport of the molecule, the ability of different compounds to reduce DHA was analyzed and their effect on ASC-transport activity tested. Administering of various reductants resulted in different levels of inhibition of ASC uptake (dithiothreitol > dithioerythritol > P-mercaptoethanol > @mercaptopropanol). Clutathione, cysteine, dithionite, and thiourea did not significantly affect ASC transport.Statistical analysis indicated a strong correlation of the Spearman rank correlation coefficient (R,) of 0.919 (P = 0.0005, n = 9) between the level of ASC oxidation and the amount of transported molecules into the vesicles. The administering of ASC oxidants such as ferricyanide and ASC oxidase resulted in a stimulated ASC uptake into the plasma membrane vesicles. Together, our results demonstrate that a vitamin C carrier in purified bean plasma membranes translocates DHA from the apoplast to the cytosol.

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Section: Discussionmentioning

confidence: 99%

The Ascorbate Carrier of Higher Plant Plasma Membranes Preferentially Translocates the Fully Oxidized (Dehydroascorbate) Molecule

1997

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“…The reaction was initiated by adding 0.01 ml extract, and increase in A470 min -) was measured. Guaiacol was used as the substrate in this assay because it reacts with more isoforms of peroxidase than many other hydrogen donors (Ievinsh 1992), and because it remains in solution more readily than other substrates during the assay. Soluble protein in the homogenate was analyzed by the Bradford (1976) method.…”

Section: Total Soluble Plus Ionically Bound Peroxidase Activitymentioning

confidence: 99%

A transient increase in apoplastic peroxidase activity precedes decrease in elongation rate of B73 maize (Zea mays) leaf blades

Souza

MacAdam

1998

Physiologia Plantarum

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Peroxidase (EC 1.11.1.7) activity from homogenized tissue or in apoplastic fluid was analyzed along the developmental gradient of expanding B73 maize (Zea mays L.) leaf blades. Soluble plus ionically bound peroxidase activity from homogenized tissue was present in high levels at the leaf base, which includes the region of cell division, and decreased as tissue was displaced away from the base by growth. A different pattern of change in peroxidase activity was seen in apoplastic fluid extracted from segments of intact tissue, where an increase in peroxidase activity preceded a rapid decrease in leaf elongation rate. Similar patterns in peroxidase activity from homogenized and intact tissue have been found in leaf blades of tall fescue (Festuca arundinacea Schreb.), suggesting a common phenomenon. At the location within the elongation zone where the increase in apoplastic peroxidase activity occurred, the activities of neutral and acidic (pl 4.6) peroxidase isoforms were also elevated in both the homogenate and in apoplastic fluid. The coincidence of these isoforms with the decline in leaf elongation rate suggests they may contribute to cessation of growth. At the distal end of the elongation zone, the activities of other acidic peroxidases (pI 5.6 and 5.7) increased in the homogenate and in apoplastic fluid, and remained elevated as tissue was displaced into the maturation region. The location of their appearance and their relatively high activity in the maturation region suggest the involvement of these isoforms in lignification.

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The cell wall stiffening mechanism inPinus pinaster Aiton: regulation by apoplastic levels of ascorbate and hydrogen peroxide

Zarra¹,

Sánchez²,

Queijeiro³

et al. 1999

J. Sci. Food Agric.

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The peroxidase activity associated with cell walls from hypocotyls of Pinus pinaster Aiton seedlings grown in the absence of light as well as cell wall content of ferulic acid, cell wall extensibility and apoplastic ascorbate and hydrogen peroxide contents are reviewed in relation to the cell wall stiffening mechanism and growth cessation. Phenolic cross‐linking, mainly 8,8′‐dehydrodiferulate, between wall polysaccharides formed by the action of wall peroxidase is the main factor involved in the wall stiffening mechanism. A regulatory mechanism for stiffening based on the relative content of ascorbate and hydrogen peroxide in the apoplast is proposed. © 1999 Society of Chemical Industry

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Characterization of the Peroxidase System in Winter Rye Seedlings:Compartmentation and Dependence on Leaf Development and Hydrogen Donors Used

Cited by 6 publications

References 20 publications

The Ascorbate Carrier of Higher Plant Plasma Membranes Preferentially Translocates the Fully Oxidized (Dehydroascorbate) Molecule

The Ascorbate Carrier of Higher Plant Plasma Membranes Preferentially Translocates the Fully Oxidized (Dehydroascorbate) Molecule

A transient increase in apoplastic peroxidase activity precedes decrease in elongation rate of B73 maize (Zea mays) leaf blades

The cell wall stiffening mechanism inPinus pinaster Aiton: regulation by apoplastic levels of ascorbate and hydrogen peroxide

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