Characterization of the phosphate-specific transport system in<i>Cronobacter sakazakii</i>BAA-894

Liang, Xiujun; Hu, Xiaoqing; Wang, Xiaoyuan; Wang, Jianli; Fang, Yu; Li, Yanyan

doi:10.1111/jam.13524

Cited by 11 publications

(3 citation statements)

References 53 publications

(102 reference statements)

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“…Since these processes depend on the presence of bacteria that accumulate large quantities of polyP, multiple attempts to engineer bacteria with increased polyP yield have been carried out. These strategies, most of them carried out in E. coli , dealt with increasing the levels of polyP kinase (PPK), encapsulating it, or increasing phosphate transport ( Kato et al, 1993 ; Keasling et al, 1998 ; Liang et al, 2017 ). However, massive polyP accumulation in a cyanobacterium is of greatest biotechnological interest and, in this context, S. elongatus has been shown to be an option for sustainable wastewater treatment ( Samiotis et al, 2021 ).…”

Section: Resultsmentioning

confidence: 99%

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

Llop

Labella²,

Borisova³

et al. 2023

Front. Microbiol.

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The cyanobacterial protein PipY belongs to the Pyridoxal-phosphate (PLP)-binding proteins (PLPBP/COG0325) family of pyridoxal-phosphate-binding proteins, which are represented in all three domains of life. These proteins share a high degree of sequence conservation, appear to have purely regulatory functions, and are involved in the homeostasis of vitamin B6 vitamers and amino/keto acids. Intriguingly, the genomic context of the pipY gene in cyanobacteria connects PipY with PipX, a protein involved in signaling the intracellular energy status and carbon-to-nitrogen balance. PipX regulates its cellular targets via protein–protein interactions. These targets include the PII signaling protein, the ribosome assembly GTPase EngA, and the transcriptional regulators NtcA and PlmA. PipX is thus involved in the transmission of multiple signals that are relevant for metabolic homeostasis and stress responses in cyanobacteria, but the exact function of PipY is still elusive. Preliminary data indicated that PipY might also be involved in signaling pathways related to the stringent stress response, a pathway that can be induced in the unicellular cyanobacterium Synechococcus elongatus PCC7942 by overexpression of the (p)ppGpp synthase, RelQ. To get insights into the cellular functions of PipY, we performed a comparative study of PipX, PipY, or RelQ overexpression in S. elongatus PCC7942. Overexpression of PipY or RelQ caused similar phenotypic responses, such as growth arrest, loss of photosynthetic activity and viability, increased cell size, and accumulation of large polyphosphate granules. In contrast, PipX overexpression decreased cell length, indicating that PipX and PipY play antagonistic roles on cell elongation or cell division. Since ppGpp levels were not induced by overexpression of PipY or PipX, it is apparent that the production of polyphosphate in cyanobacteria does not require induction of the stringent response.

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Section: Resultsmentioning

confidence: 99%

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

Llop

Labella²,

Borisova³

et al. 2023

Front. Microbiol.

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“…Cronobacter species are food-borne opportunistic pathogens that can cause necrotizing enterocolitis and meningitis in neonates and infants ( Feeney et al, 2014 ; Forsythe, 2017 ). Cronobacter sakazakii , C. dublinensis , C. malonaticus , and C. turicensis are the common Cronobacter isolates, especially C. sakazakii which has been extensively studied ( Zhang et al, 2014 ; Liang et al, 2017 ; Li et al, 2017 ). C. sakazakii is much more resistant than other Enterobacteriaceae to environmental stresses ( Dancer et al, 2009 ), which might be associated with its pathogenesis ( Alvarez-Ordóñez et al, 2014 ).…”

Section: Introductionmentioning

confidence: 99%

The Global Response of Cronobacter sakazakii Cells to Amino Acid Deficiency

et al. 2018

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Cronobacter species can cause necrotizing enterocolitis and meningitis in neonates and infants, their infection is closely relevant to their responses to extreme growth conditions. In this study, the response of Cronobacter species to amino acid deficiency has been investigated. Four Cronobacter species formed smooth colonies when grown on the solid LB medium, but formed mucoid colonies when grown on the amino acid deficient M9 medium. When the mucoid colonies were stained with tannin mordant, exopolysaccharide around the cells could be discerned. The exopolysaccharide was isolated, analyzed, and identified as colanic acid. When genes wcaD and wcaE relevant to colanic acid biosynthesis were deleted in Cronobacter sakazakii BAA-894, no exopolysaccharide could be produced, confirming the exopolysaccharide formed in C. sakazakii grown in M9 is colanic acid. On the other hand, when genes rcsA, rcsB, rcsC, rcsD, or rcsF relevant to Rcs phosphorelay system was deleted in C. sakazakii BAA-894, colanic acid could not be produced, suggesting that the production of colanic acid in C. sakazakii is regulated by Rcs phosphorelay system. Furthermore, C. sakazakii BAA-894 grown in M9 supplemented with amino acids could not produce exopolysaccharide. Transcriptomes of C. sakazakii BAA-894 grown in M9 or LB were analyzed. A total of 3956 genes were differentially expressed in M9, of which 2339 were up-regulated and 1617 were down-regulated. When C. sakazakii BAA-894 was grown in M9, the genes relevant to the biosynthesis of exopolysaccharide were significantly up-regulated; on the other hand, the genes relevant to the flagellum formation and chemotaxis were significantly down-regulated; in addition, most genes relevant to various amino acid biosynthesis were also significantly regulated. The results demonstrate that amino acid deficiency has a global impact on C. sakazakii cells.

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“…In addition to its role in phosphate homeostasis, the Pho regulon has been connected to bacterial virulence and biofilm formation in several species, including Pseudomonas aeruginosa, P. aureofaciens, P. fluorescens, Agrobacterium tumefaciens, Vibrio cholerae, Bacillus anthracis, and Cronobacter sakazakii (12)(13)(14)(15)(16)(17)(18)(19). Recent studies established that EHEC strains are able to form biofilms in different environments, including food surfaces, processing plants, and water (20,21).…”

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confidence: 99%

Regulation of waaH by PhoB during P _i Starvation Promotes Biofilm Formation by Escherichia coli O157:H7

et al. 2019

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In open environments such as water, enterohemorrhagic Escherichia coli O157:H7 responds to inorganic phosphate (Pi) starvation by inducing the Pho regulon controlled by PhoB. This activates the phosphate-specific transport (Pst) system that contains a high-affinity Pi transporter. In the Δpst mutant, PhoB is constitutively activated and regulates the expression of genes in the Pho regulon. Here, we show that Pi starvation and deletion of the pst system enhance E. coli O157:H7 biofilm formation. Among differentially expressed genes of EDL933 grown under Pi starvation conditions and in the Δpst mutant, we have found that a member of the PhoB regulon, waaH, predicted to encode a glycosyltransferase, was highly expressed. Interestingly, WaaH contributed to biofilm formation of E. coli O157:H7 during both Pi starvation and in the Δpst mutant. In the Δpst mutant, the presence of waaH was associated with lipopolysaccharide (LPS) R3 core type modifications, whereas in E. coli O157:H7, waaH overexpression had no effect on LPS structure during Pi starvation. Therefore, waaH participates in E. coli O157:H7 biofilm formation during Pi starvation, but its biochemical role remains to be clarified. This study highlights the importance of the Pi starvation stress response to biofilm formation, which may contribute to the persistence of E. coli O157:H7 in the environment. IMPORTANCE Enterohemorrhagic Escherichia coli O157:H7 is a human pathogen that causes bloody diarrhea that can result in renal failure. Outside of mammalian hosts, E. coli O157:H7 survives for extended periods of time in nutrient-poor environments, likely as part of biofilms. In E. coli K-12, the levels of free extracellular Pi affect biofilm formation; however, it was unknown whether Pi influences biofilm formation by E. coli O157:H7. Our results show that upon Pi starvation, PhoB activates waaH expression, which favors biofilm formation by E. coli O157:H7. These findings suggest that WaaH is a target for controlling biofilm formation. Altogether, our work demonstrates how adaptation to Pi starvation allows E. coli O157:H7 to occupy different ecological niches.

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Characterization of the phosphate-specific transport system inCronobacter sakazakiiBAA-894

Abstract: This study demonstrates that phosphate availability has a global influence to C. sakazakii, expends our understanding to the phosphate transfer in C. sakazakii, and is helpful for revealing the survival mechanism of C. sakazakii under stress conditions.

Cited by 11 publications

References 53 publications

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

The Global Response of Cronobacter sakazakii Cells to Amino Acid Deficiency

Regulation of waaH by PhoB during P _i Starvation Promotes Biofilm Formation by Escherichia coli O157:H7

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Characterization of the phosphate-specific transport system inCronobacter sakazakiiBAA-894

Abstract: This study demonstrates that phosphate availability has a global influence to C. sakazakii, expends our understanding to the phosphate transfer in C. sakazakii, and is helpful for revealing the survival mechanism of C. sakazakii under stress conditions.

Cited by 11 publications

References 53 publications

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

Pleiotropic effects of PipX, PipY, or RelQ overexpression on growth, cell size, photosynthesis, and polyphosphate accumulation in the cyanobacterium Synechococcus elongatus PCC7942

The Global Response of Cronobacter sakazakii Cells to Amino Acid Deficiency

Regulation of waaH by PhoB during P i Starvation Promotes Biofilm Formation by Escherichia coli O157:H7

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Regulation of waaH by PhoB during P _i Starvation Promotes Biofilm Formation by Escherichia coli O157:H7