Characterization of the Potential Subpopulation of Bone Marrow Cells Involved in the Repair of Injured Liver Tissue

Abstract

ABSTRACT

Hepatocyte Nuclear Factor-4α Induces Transdifferentiation of Hematopoietic Cells into Hepatocytes

“…However, none of the earlier studies (14 -23) described the molecular basis of transdifferentiation. Previously, we have described an in vitro culture system for transdifferentiation of lineage-depleted oncostatin M receptor ␤-expressing (Lin Ϫ OSMR␤ ϩ ) BM cells into hepatocytes (18,23). In this study, we report that HNF4␣ acts as a molecular switch for transdifferentiation of BM-derived cells into hepatocytes.…”

“…The purified cells were cultured in LabTek chamber slides as well as in 6-well plates at a cell density of 5 ϫ 10 4 cells/cm 2 in the presence of Iscove's modified Dulbecco's medium supplemented with 10% damaged liver serum (DLS) and 1 M dexamethasone (23). The DLS was collected from the mice 24 h after intraperitoneal injection of a 10% carbon tetrachloride solution (200 l) in mineral oil (18). Murine myeloid cells (32Dcl3) were cultured in RPMI 1640 medium supplemented with 10% fetal calf serum and 5 ng/ml interleukin-3 (PeproTech Asia, Israel).…”

Treatment of the end Stage Liver Cirrhosis by Human Umbilical Cord Blood Stem Cells: Preliminary Results

“…For advanced liver diseases such as cirrhosis, stem cell engraftment can be most promising strategy after the organ transplantation (146). In animals treated with CCl 4 intraperitoneal infusion, the percentage of lin-Sca-1+ cells in the bone marrow and peripheral blood increases twofold at twenty-four hours later (147) and this increase peaks at day one in the bone marrow and day two in the peripheral blood (147). This finding suggests that bone stem cells started to proliferate and migrate to the periphery (147 (165) and sometimes via specific route such as portal vein (166).…”

“…In animals treated with CCl 4 intraperitoneal infusion, the percentage of lin-Sca-1+ cells in the bone marrow and peripheral blood increases twofold at twenty-four hours later (147) and this increase peaks at day one in the bone marrow and day two in the peripheral blood (147). This finding suggests that bone stem cells started to proliferate and migrate to the periphery (147 (165) and sometimes via specific route such as portal vein (166). And in case of cellular cardioplasty for myocardiac infarction model, several different infusion has been used, direct intracoronary (167), intravenous with homing(168) and mobilization with homing from bone marrow or peripheral blood (169).…”

Methods of Liver Stem Cell Therapy in Rodents as Models of Human Liver Regeneration in Hepatic Failure

“…17 Injection of doses between 0.5-1 mL/kg CCl4 dissolved in olive [18][19][20] or corn oil 5,21 twice a week for 4-8 weeks 18,22 have been used for liver injury in small animal models. 12,20 CCl4 can be injected to rodents intraperitoneally 12,23,24 or subcutaneously. 5,18,20,21 Paracetamol, 25 ally alcohol (AA) 26 and dimethylnitroseamine (DMN) are other chemical agents for inducing liver damage in rodents.…”