Characterization of the structure, stability, and activity of hypoglycemic peptides from<i>Moringa oleifera</i>seed protein hydrolysates

Wang, Xuefeng; Fan, Yaozhu; Xu, Feiran; Xie, Jing; Gao, Xiaoyu; Li, Lingfei; Yang, Tian; Sheng, Jun

doi:10.1039/d1fo03413h

Cited by 24 publications

(13 citation statements)

References 52 publications

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“…Similarly, we previously studied the in vitro hypoglycemic activity and stability of MOP, and found that after MOP was digested by pepsin, it still had an inhibitory effect on a-glucosidase activity, with an inhibition rate of 97.60%. And after MOP was digested by trypsin, it still had inhibitory effect on a-glucosidase activity, and the inhibition rate was 86.76% (26). Through the above studies, we believe that MOP has a certain resistance to pepsin and trypsin, and has good stability.…”

Section: Discussionmentioning

confidence: 87%

“…Currently, it is unclear whether M. oleifera seed-derived peptides are also anti-inflammatory, and if so, whether they modulate gut microbiota and regulate metabolism in vivo to exert their anti-inflammatory effects in vivo, especially vis-à-vis acute colitis. In earlier work, we had identified the ultrafiltration peptide components (i.e., < 3 kDa) of M. oleifera seeds protein hydrolysates, finding a highly active aglucosidase inhibitory peptide with the amino acid sequence KETTTIVR that also exhibited anti-inflammatory activity in vitro (26). Yet whether it has anti-UC activity in vivo and its mechanism of action remain unclear.…”

Section: Introductionmentioning

confidence: 99%

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Moringa oleifera Lam. Peptide Remodels Intestinal Mucosal Barrier by Inhibiting JAK-STAT Activation and Modulating Gut Microbiota in Colitis

et al. 2022

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Ulcerative colitis is a chronic inflammatory bowel disease (IBD), but progress in exploring its pathogenesis and finding effective drugs for its prevention and treatment has stalled in recent years. The seeds of Moringa oleifera Lam. are rich in proteins known to have multiple physiological activities. In our earlier work, we had isolated and purified a peptide (MOP) having the sequence KETTTIVR, from M. oleifera seeds; however, its anti-inflammatory activity and mechanism in vivo were unclear. Here we used the dextran sulfate sodium (DSS)-induced colitis model to study the anti-inflammatory activity and mechanism of this MOP. Our results are the first to show that MOP can ameliorate the pathological phenotype, inflammation, and intestinal barrier disruption in mice with colitis. Furthermore, RNA sequencing revealed that MOP inhibits the Janus kinase/signal transducer and activator of transcription (JAK-STAT) pathway activation. Next, by using 16s rRNA gene sequencing, we found that MOP can ameliorate DSS-induced gut microbiota dysbiosis. In addition, an untargeted metabolomics analysis suggested that MOP is able to modulate the level of lipid and amino acid metabolites in IBD-stricken mice. Altogether, these results indicate that MOP ameliorates colitis by remodeling intestinal mucosal barrier by inhibiting JAK-STAT pathway’s activation and regulating gut microbiota and its metabolites, thus providing a basis for further processing and design of bioactive foods from M. oleifera seeds.

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Section: Discussionmentioning

confidence: 87%

Section: Introductionmentioning

confidence: 99%

Moringa oleifera Lam. Peptide Remodels Intestinal Mucosal Barrier by Inhibiting JAK-STAT Activation and Modulating Gut Microbiota in Colitis

et al. 2022

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“…The seeds of the plant exhibit various biological activities like, antidiabetic [ 31 ], anti-inflammatory [ 32 ], hepatoprotective [ 33 ], anticancer [ 34 ], cardiovascular, CNS, analgesic [ 35 ], wound healing [ 36 ], antiallergic, antifertility, antiasthmatic [ 37 ], antiulcer, and antipyretic activity [ 38 ]. Therefore, this study was designed to evaluate the antioxidant potential of M. oleifera seeds of two different accessions from different regions.…”

Section: Introductionmentioning

confidence: 99%

Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts

Tariq

Umbreen

Noreen

et al. 2022

BioMed Research International

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Medicinal plants are used to control and remediate oxidative stress related diseases caused by free radicals. Thus, these plants find their use as remedy. Moringa oleifera is an extremely valued plant for its medicinal properties. Herein, two indigenously produced accessions of Moringa oleifera seeds [originated from Multan (M-Mln) and India (PKM1)] were investigated for their antioxidant properties by 2.2-Diphenyl-1picrylhydrazyl (DPPH) assay, total phenolics content and total flavonoids content. The presence of various phenolics as well as flavonoids was further confirmed by high performance liquid chromatography. Moreover, fourier transform infrared spectroscopy detected the presence of various functional groups. In conclusion, these findings revealed that the methanol extract of M-Mln variety seeds showed high antioxidant potential, having IC50 value of 84 μg/ml. While, hexane extract of PKM1 showed least activity. The methanol extract of M-Mln was found to show highest total phenolics content as 33.83 ± 1.19 mg GAE/g. The methanol extract of M-Mln was found to show highest total flavonoids content as 76.07 ± 1.10 mg CAE/g. The hexane extract of PKM1 was found to show least total flavonoids content as 22.47 ± 1.70 mg CAE/g. The detection of phenolics (ferulic acid, caffeic acid, chlorogenic acid, coumaric acid, and gallic acid) as well as flavonoids (catechin and quercetin) revealed the potential of methanol extracts of both varieties as a good source of antioxidants. The results indicated the importance of seed extracts in the treatment of oxidative stress related diseases. In future, the use of natural antioxidants will prevent the progression of diseases.

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“…These two peptides exhibited superior hydrophobicity and biological activity compared to the other peptides. Studies have shown that amino acid sequences containing leucine, proline, tyrosine, and valine can participate in blood sugar control, enhancing the hypoglycemic activity of peptides ( 38 ). Considering these factors, we chose the peptide FYLPKM for further research, as it may be an advantageous peptide for hypoglycemic activity.…”

Section: Resultsmentioning

confidence: 99%

Preparation of sea buckthorn (Hippophae rhamnoides L.) seed meal peptide by mixed fermentation and its effect on volatile compounds and hypoglycemia

Yang,

Hong,

Aihaiti

et al. 2024

Front. Nutr.

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This study employed mixed bacterial strains to ferment seabuckthorn seed meal into peptides, and conducted a comprehensive evaluation of the growth adaptive conditions, molecular weight distribution, volatile compounds, and in vitro hypoglycemic activity required for fermentation. Results showed that when the amount of maltose was 1.1% and MgSO4·7H2O was added at 0.15 g/L, the peptide yield reached 43.85% with a mixed fermentation of Lactobacillus fermentum, Bacillus subtilis, Lactobacillus casei, Lactobacillus rhamnosus, and Lactobacillus acidophilus. Components with a molecular weight below 1 kDa were found to be more effective in inhibiting the activity of α-amylase and α-glucosidase, with the identified sequence being FYLPKM. Finally, SPME/GC–MS results showed that 86 volatile components were detected during the fermentation of seabuckthorn seed meal, including 22 alcohols, 9 acids, 7 ketones, 14 alkanes, 20 esters, and 14 other compounds. With prolonged fermentation time, the content of acids and esters increased significantly.

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Characterization of the structure, stability, and activity of hypoglycemic peptides fromMoringa oleiferaseed protein hydrolysates

Abstract: Moringa oleifera seed protein hydrolysates show good hypoglycemic activity, but their specific peptide components have not yet been characterized. Here, we identified the ultrafiltration peptide components (<3 kDa) of M....

Cited by 24 publications

References 52 publications

Moringa oleifera Lam. Peptide Remodels Intestinal Mucosal Barrier by Inhibiting JAK-STAT Activation and Modulating Gut Microbiota in Colitis

Moringa oleifera Lam. Peptide Remodels Intestinal Mucosal Barrier by Inhibiting JAK-STAT Activation and Modulating Gut Microbiota in Colitis

Comparative Analysis of Antioxidants Activity of Indigenously Produced Moringa Oleifera Seeds Extracts

Preparation of sea buckthorn (Hippophae rhamnoides L.) seed meal peptide by mixed fermentation and its effect on volatile compounds and hypoglycemia

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