Characterization of the traC determinant of the Enterococcus faecalis hemolysin-bacteriocin plasmid pAD1: binding of sex pheromone

Tanimoto, Koichi; An, Florence Y.; Clewell, Don B.

doi:10.1128/jb.175.16.5260-5264.1993

Cited by 80 publications

(59 citation statements)

References 33 publications

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“…Previous analysis of the biological activities of various peptide pheromones and inhibitors (23) indicated that the iAD1 peptide, which acts as an inhibitor in the pADi system, had a very low level of pheromone activity against cells carrying pCF10. This previous observation, as well as the similarity of the two binding proteins (34), may account for the observation that pADi confers a slight ability to bind cCF10.…”

Section: Discussionmentioning

confidence: 86%

“…The pADi traC and the pCF10 prgZ gene products show a high degree of similarity, with almost complete identity in the amino-terminal halves of the proteins (34).…”

Section: Resultsmentioning

confidence: 99%

“…In the accompanying paper from Tanimoto et al (34), the sequence of the traC gene of pADi is presented, along with evidence that traC encodes a binding function for the pheromone cAD1. The pADi traC and the pCF10 prgZ gene products show a high degree of similarity, with almost complete identity in the amino-terminal halves of the proteins (34).…”

Section: Resultsmentioning

confidence: 99%

“…With regard to the latter hypothesis, several adjacent genes in the region ofprgZ, includingprgWprgY, andprgX, could play a role. All three of these genes have homologs in the pADi system (1,7,34), and in some cases, genetic evidence suggesting a role for some of these genes in signal transduction has been obtained (7,34). The fact that deletions from the 5' end ofprgW that removed all or part of this gene but left the putative prgZ promoter intact resulted in instability in an E. faecalis host could indicate that the prgW product interacts with the prgZ promoter region.…”

Section: Discussionmentioning

confidence: 99%

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Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function

1993

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In order to investigate the mechanism by which peptide sex pheromones induce expression of the conjugation functions of certain Enterococcus faecalis plasmids, a biological assay was developed to measure the ability of cells carrying the conjugative plasmid pCF10 to bind the sex pheromone cCF10. The data indicated that pCF10 endows its host E. faecalis cell with the ability to specifically remove (apparently by irreversible binding) cCF10 activity from culture medium. The pCF10 DNA encoding this ability was localized to a 3.4-kb segment within a region involved in negative control of expression of conjugal transfer functions. This segment also encoded ability to bind the pheromone inhibitor peptide iCF10. DNA sequencing revealed three open reading frames, which have been denoted prgW (pheromone responsive gene W), prgZ, and prgY. The deduced product of prgW resembled regulatory proteins from other bacteria and eucaryotes, with a very high degree of identity within a putative DNA-binding domain. The prgY gene actually extended into an adjacent region of pCF10 and could encode a protein with significant similarity to a protein called TraB, believed to be involved in shutdown of pheromone cAD1 production by cells carrying the pheromone-inducible hemolysin plasmid pAD1, according to F.Y. An and D.B. Clewell (Abstr. Gen. Meet. Am. Soc. Microbiol. 1992, H70, 1992). The prgZ gene product showed significant relatedness to binding proteins encoded by oligopeptide permease (opp) operons in gram-positive and gram-negative bacteria and is highly similar to a pAD1-encoded protein, TraC, which is believed to mediate sex pheromone cAD1 binding (K. Tanimoto, F. Y. An, and D. B. Clewell, submitted for publication). A Tn5 insertion into prgZ abolished cCF10 binding ability.

show abstract

Section: Discussionmentioning

confidence: 86%

“…The pADi traC and the pCF10 prgZ gene products show a high degree of similarity, with almost complete identity in the amino-terminal halves of the proteins (34).…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

See 2 more Smart Citations

Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function

1993

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“…TraC is a surface lipoprotein that represents the specific receptor for exogenous cAD1, 142 and is believed to be associated with a membranous host-encoded peptide uptake system. 143 The plasmid-encoded inhibitor peptide iAD1 is believed to act competitively with cAD1 at the receptor to de-sensitize donor cells to endogenously produced pheromone.…”

mentioning

confidence: 99%

Tales of conjugation and sex pheromones

Clewell¹

2011

Mobile Genetic Elements

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REVIEW REVIEW use of a technique that had recently been reported by Godson and Sinsheimer 6 that utilized a detergent mixture of Brij 58 (a neutral detergent) and deoxycholate for lysis of phageinfected E. coli. During a relatively low-speed centrifugation step, the vast majority of chromosomal DNA pelleted leaving the supernatant greatly enriched in plasmid DNA. Sucrose gradient analyses of these "cleared lysates" revealed a ColE1-protein complex sedimenting slightly faster (24S) than the 23S protein-free supercoiled DNA. Surprisingly, when the 24S complex was exposed to conditions expected to affect protein structure such as proteases, strong ionic detergents or heat, the 24S complex converted to a 17S relaxed (open circular) configuration. The relaxation event involved the introduction of a strand-specific nick in the supercoiled plasmid; we therefore called the 24S entity a "relaxation complex". 7,8 The percentage of plasmid DNA that was present in the complexed state varied from less than 30%, when glucose was in the growth medium, to more than 80% when glucose was absent.

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Enterococcus

Teixeira¹,

Facklam²

2010

Topley &Amp; Wilson's Microbiology and Microbial Infections

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Characterization of the traC determinant of the Enterococcus faecalis hemolysin-bacteriocin plasmid pAD1: binding of sex pheromone

Cited by 80 publications

References 33 publications

Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function

Cloning and characterization of a region of the Enterococcus faecalis conjugative plasmid, pCF10, encoding a sex pheromone-binding function

Tales of conjugation and sex pheromones

Enterococcus

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