Characterization of Thylakoid Division Using Chloroplast Dividing Mutants in<i>Arabidopsis</i>

Ho, Jason; Kwan, Warren; Li, Vivian; Theg, Steven M.

doi:10.1101/200667

Cited by 2 publications

(1 citation statement)

References 52 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…[ 1 ] Chloroplast is surrounded by inner and outer membranes. Inside the chloroplast, there are some columns of stacked discs named granum and each part of these columns is called thylakoid [ 2 ] with 300–600 nm diameter. [ 3 ] Two important parts of photosynthesis, namely, photosystem I (PSI) and photosystem II (PSII), exist in the thylakoid membrane.…”

Section: Introductionmentioning

confidence: 99%

Absorption Analysis of Photosystem I in Different Plants for Biohybrid Solar Cell Applications

et al. 2022

View full text Add to dashboard Cite

Biohybrid solar cells, which are inspired by photosynthesis process in plants, are new low‐cost and environmentally friendly solar cells. In this kind of solar cells, photosystem I (PSI), which is the main part of photosynthesis process, is used as light absorber layer. Herein, PSI is extracted from some easily accessible plants, including spinach, Tung‐Oh, chye sim, local endive, beet greens, leek, Swiss chard, and romaine lettuce, and their light absorbance properties are studied. It is shown that the optical bandgap of PSI extracted from all of these plants is about 1.8 eV which indicates the potential of these plants for use in biohybrid solar cells. It is shown that, due to the higher light absorbance, spinach is the best plant for this kind of solar cells. Also, the effect of temperature on light absorbance of PSI extracted from spinach is investigated and shows that the light absorbance of PSI considerably decreases at high‐temperature values. Furthermore, alkaline and acidic environments on PSI light absorbance are studied and shows that the alkaline pH stress has no destructive effect on light absorbance, while the acidic pH stress causes a considerable decrease in light absorbance which is important in biohybrid solar cell fabrication.

show abstract

Section: Introductionmentioning

confidence: 99%

Absorption Analysis of Photosystem I in Different Plants for Biohybrid Solar Cell Applications

et al. 2022

View full text Add to dashboard Cite

show abstract

The Arabidopsis arc5 and arc6 mutations differentially affect plastid morphology in pavement and guard cells in the leaf epidermis

et al. 2018

View full text Add to dashboard Cite

Chloroplasts, or photosynthetic plastids, multiply by binary fission, forming a homogeneous population in plant cells. In Arabidopsis thaliana, the division apparatus (or division ring) of mesophyll chloroplasts includes an inner envelope transmembrane protein ARC6, a cytoplasmic dynamin-related protein ARC5 (DRP5B), and members of the FtsZ1 and FtsZ2 families of proteins, which co-assemble in the stromal mid-plastid division ring (FtsZ ring). FtsZ ring placement is controlled by several proteins, including a stromal factor MinE (AtMinE1). During leaf mesophyll development, ARC6 and AtMinE1 are necessary for FtsZ ring formation and thus plastid division initiation, while ARC5 is essential for a later stage of plastid division. Here, we examined plastid morphology in leaf epidermal pavement cells (PCs) and stomatal guard cells (GCs) in the arc5 and arc6 mutants using stroma-targeted fluorescent proteins. The arc5 PC plastids were generally a bit larger than those of the wild type, but most had normal shapes and were division-competent, unlike mutant mesophyll chloroplasts. The arc6 PC plastids were heterogeneous in size and shape, including the formation of giant and mini-plastids, plastids with highly developed stromules, and grape-like plastid clusters, which varied on a cell-by-cell basis. Moreover, unique plastid phenotypes for stomatal GCs were observed in both mutants. The arc5 GCs rarely lacked chlorophyll-bearing plastids (chloroplasts), while they accumulated minute chlorophyll-less plastids, whereas most GCs developed wild type-like chloroplasts. The arc6 GCs produced large chloroplasts and/or chlorophyll-less plastids, as previously observed, but unexpectedly, their chloroplasts/plastids exhibited marked morphological variations. We quantitatively analyzed plastid morphology and partitioning in paired GCs from wild-type, arc5, arc6, and atminE1 plants. Collectively, our results support the notion that ARC5 is dispensable in the process of equal division of epidermal plastids, and indicate that dysfunctions in ARC5 and ARC6 differentially affect plastid replication among mesophyll cells, PCs, and GCs within a single leaf.

show abstract

Characterization of Thylakoid Division Using Chloroplast Dividing Mutants inArabidopsis

Abstract: 24Chloroplasts are double membrane bound organelles that are found in plants and algae. Their

Cited by 2 publications

References 52 publications

Absorption Analysis of Photosystem I in Different Plants for Biohybrid Solar Cell Applications

Absorption Analysis of Photosystem I in Different Plants for Biohybrid Solar Cell Applications

The Arabidopsis arc5 and arc6 mutations differentially affect plastid morphology in pavement and guard cells in the leaf epidermis

Contact Info

Product

Resources

About