Characterization of Transcriptome Expression: The Response of Isatis indigotica to Salt Stress

Tang, Xiao‐Qing; Tan, Honglue; Shi, Song; Wang, L.-L.; Wang, F.-Q.; Wang, K.-C.; Yang, Jie

doi:10.1134/s1021443720060163

Cited by 1 publication

(1 citation statement)

References 25 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…is an herbaceous plant belonging to the Cruciferae family; its leaf (IIL) and root (IIR) are commonly used in traditional Chinese medicines, with anti-inflammatory, analgesic, and antioxidant effects [ 18 , 19 , 20 ]. Previous studies have focused on the growth, photosynthetic parameters, and antioxidant enzyme responses of I. indigotica under salt stress [ 21 ], and changes in transcriptomics [ 22 ]. However, there are few studies on the growth morphology, mesophyll-cell ultrastructure and root-section changes of Isatis indigotica Fort.…”

Section: Introductionmentioning

confidence: 99%

Physiological and Cellular Ultrastructural Responses of Isatis indigotica Fort. under Salt Stress

Wu,

Jia,

Tian

et al. 2024

Plants

View full text Add to dashboard Cite

This study aimed to analyze the effects of salt stress on the growth physiology and plant-cell ultrastructure of Isatis indigotica Fort. (I. indigotica) to evaluate its adaptability under salt stress. The effects of different concentrations of salt (NaCl; 0, 25, and 300 mmol·L−1) on the agronomic traits, activities of related enzymes, ion balance, and mesophyll-cell ultrastructure of I. indigotica were studied in a controlled pot experiment. Results showed that compared with those of the control group, the aerial-part fresh weight, underground fresh weight, tiller number, root length, root diameter, plant height, and leaf area of salt-stressed I. indigotica increased at 25 mmol·L−1 and then decreased at 300 mmol·L−1. The changes in levels of superoxide dismutase, peroxidase, ascorbate peroxidase, and catalase showed a similar trend, with significant differences compared with control group. Salt stress altered the ion balance of I. indigotica, resulting in a significant increase in Na+ content and a significant decrease in K+ content. The contents of Ca2+ and Mg2+ changed to varying degrees. The analysis of the microstructure of the root showed that under salt treatment, the epidermal cells of the root significantly thickened and the diameter of the xylem decreased. The results of ultrastructural analysis of mesophylls showed that salt stress can cause cell-membrane contraction, cell-gap enlargement, disorder in the structures of chloroplasts and mitochondria, and an increase in the number of osmiophilic particles. These changes were aggravated by the increase in NaCl concentration. This study reveals the response of I. indigotica to salt stress and provides a basis for further study on the salt-tolerance mechanism of I. indigotica.

show abstract