Characterization of two trpE genes encoding anthranilate synthase α-subunit in Azospirillum brasilense

Ge, Shimei; Xie, Baoen; Chen, Sanfeng

doi:10.1016/j.bbrc.2006.01.009

Cited by 7 publications

(9 citation statements)

References 20 publications

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“…This novel pathway could be involved in different functions that require either tryptophan or anthranilate as intermediates. Examples of these functions are quorum sensing molecules in Pseudomonas aeruginosa (Farrow & Pesci, 2007) , plant hormones in Azospirillum brasilense (Ge, Xie & Chen, 2006), violacein in Chromobacterium violacein (Antônio & Creczynski-Pasa, 2004) or antibiotics as in Streptomyces coelicolor (Amir-Heidari, Thirlway & Micklefield, 2008) .…”

Section: Resultsmentioning

confidence: 99%

The genomic sequence ofExiguobacterium chiriqhuchastr. N139 reveals a species that thrives in cold waters and extreme environmental conditions

Gutiérrez-Preciado

Vargas-Chávez

Reyes-Prieto

et al. 2017

PeerJ

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We report the genome sequence of Exiguobacterium chiriqhucha str. N139, isolated from a high-altitude Andean lake. Comparative genomic analyses of the Exiguobacterium genomes available suggest that our strain belongs to the same species as the previously reported E. pavilionensis str. RW-2 and Exiguobacterium str. GIC 31. We describe this species and propose the chiriqhucha name to group them. ‘Chiri qhucha’ in Quechua means ‘cold lake’, which is a common origin of these three cosmopolitan Exiguobacteria. The 2,952,588-bp E. chiriqhucha str. N139 genome contains one chromosome and three megaplasmids. The genome analysis of the Andean strain suggests the presence of enzymes that confer E. chiriqhucha str. N139 the ability to grow under multiple environmental extreme conditions, including high concentrations of different metals, high ultraviolet B radiation, scavenging for phosphorous and coping with high salinity. Moreover, the regulation of its tryptophan biosynthesis suggests that novel pathways remain to be discovered, and that these pathways might be fundamental in the amino acid metabolism of the microbial community from Laguna Negra, Argentina.

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Section: Resultsmentioning

confidence: 99%

The genomic sequence ofExiguobacterium chiriqhuchastr. N139 reveals a species that thrives in cold waters and extreme environmental conditions

Gutiérrez-Preciado

Vargas-Chávez

Reyes-Prieto

et al. 2017

PeerJ

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“…Escherichia coli strains were grown in LB medium at 37°C (Sambrook and Russell 2001). Azospirillum brasilense was grown at 30°C in LD medium (Ge et al 2006) and minimal K-lactate medium with 0.5 mM glutamine (Franche and Elmerich 1981;Hartmann et al 1988). Sinorhizobium meliloti strains were grown in TY or FY medium (Wei et al 2004).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Functional analysis of the fixL/fixJ and fixK genes in Azospirillum brasilense Sp7

Ren

et al. 2010

Ann Microbiol

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This study demonstrates for the first time that the two-component FixL/FixJ system, activating expression of nitrogen fixation genes in response to low oxygen concentration in rhizobia, also exists in Azospirillum brasilense Sp7. A heme staining assay showed that A. brasilense FixL contains a heme cofactor like that of rhizobial FixL proteins. Azospirillum brasilense FixL and FixJ could activate expression of Sinorhizobium meliloti nifA in S. meliloti fixL − and fixJ − mutants and in Escherichia coli, and that activation was dependent on oxygen concentration, indicating that A. brasilense FixL and FixJ have the same function as S. meliloti FixL and FixJ. Azospirillum brasilense FixL and FixJ could activate expression of A. brasilense fixK in E. coli. Azospirillum brasilense FixL, FixJ, and FixK positively regulated expression of A. brasilense nifA in A. brasilense strains but not in E. coli. The results show that FixL and FixJ can activate the fixK expression, with FixK subsequently activating nifA expression in A. brasilense. Azospirillum brasilense fixJ was involved in aerotaxis and S. meliloti fixJ could restore aerotaxis of the A. brasilense fixJ − mutant. Mutations in fixL and fixJ did not affect nitrogen fixation.

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“…glycinea PG4180, a model for studying COR biosynthesis (provided by Dr. C. L. Bender, Professor, Oklahoma State University, Stillwater, OK 74078, USA). The plasmid pPHU281, carrying a tetracyclineresistance gene, was used as a suicide vector for transforming PG4180 with homologous DNA fragments (Hübner et al 1993;Ge et al 2006). Plasmid pET30a ?…”

Section: Bacterial Strains and Plasmidsmentioning

confidence: 99%

“…Lac expression vector, Kan r Novagen pPHU281 tet r Suicide vector Hübner et al (1993) pHP45X ap r , spc r Vector provided X segment Prentki and Krisch (1984) pUC19X amp r carrying LacZ and X This study DNA sequencing were performed following Ge et al (2006).…”

Section: Dna Manipulationsmentioning

confidence: 99%

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Effects of dapA gene deletion on coronatine biosynthesis in Pseudomonas syringae pv. glycinea PG4180

Zhang

Liu

et al. 2010

World J Microbiol Biotechnol

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Coronatine (COR) is a structural and functional analogue of jasmonic acid that might be employed in agriculture to elicit plant resistance against various aggressors. However, the yield of COR is low both in chemosynthesis and biosynthesis, so broad investigation of COR is difficult. Coronatine combines two distinct components: coronafacic acid (CFA) and coronamic acid (CMA). Synthesis of both CMA and CFA is involved in Lisoleucine metabolism, so the objective of this work was to investigate if COR production can be improved by regulating amino acid biosynthesis in P. syringae pv. glycinea. Inhibition of dihydrodipicolinate synthase was achieved by removing the dapA gene via homologous recombination, which resulted in a COR yield by the dapA -mutant of about 1.5-fold greater than the wild strain. Thus, regulation of amino acid metabolism is a feasible way to increase COR production, which could be a more effective method than adding substrates into culture medium.

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Characterization of two trpE genes encoding anthranilate synthase α-subunit in Azospirillum brasilense

Cited by 7 publications

References 20 publications

The genomic sequence ofExiguobacterium chiriqhuchastr. N139 reveals a species that thrives in cold waters and extreme environmental conditions

The genomic sequence ofExiguobacterium chiriqhuchastr. N139 reveals a species that thrives in cold waters and extreme environmental conditions

Functional analysis of the fixL/fixJ and fixK genes in Azospirillum brasilense Sp7

Effects of dapA gene deletion on coronatine biosynthesis in Pseudomonas syringae pv. glycinea PG4180

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