Characterization of volatile components from ethyl acetate extract of Stixis suaveolens (Roxb.) by comprehensive two‐dimensional gas chromatography hyphenated with a time‐of‐flight mass spectrometer

Abstract

This article established a GC-MS profile for ethylacetate extract of herbal plant Stixis suaveolens (Roxb.) Pierre (Capparaceae). The plant was extracted with ethanol then partitioned by ethyl acetate. The ethyl acetate extract was analyzed using comprehensive two-dimensional gas chromatography coupling with a time-of-flight mass spectrometer (GCxGC/TOF-MS). Most of high intensity compounds in ethyl acetate extract were identified. 48 compounds out of 106 peaks were detected including fatty acids, esters, aldehydes, ketones, alcohols, heterocyclic compounds, etc. Many of them show biological activities in diseases treatment. This result facilitates further studies about herbal plants and development of medicine formulation based on traditional medicine in Vietnam.

Recent Development of a Traditional Herbal Medicine, Stixis suaveolens (Roxb.) Pierre: Its Botany, Ethnomedicine, Phytochemistry, Pharmacology, and Toxicology

“…Many compounds of S. suaveolens have been isolated from the leaves and fruits of this plant, including lignan and its glycoside derivatives, phenolics and phenolic glycosides, alkaloids, and volatile components. 6,14,16,26,29,30 S. suaveolens leaves and fruits contain various phytochemicals and nutrients, which differ in their composition, phytochemical structures, molecular weight, polarity, and other characteristics. A total of 73 phytocompounds have been determined and characterized.…”

“…In a previous study by Anh et al, 14 a silica gel column chromatography technique was carried out to isolate the significant compounds after the EA fraction was obtained by reversed-phase column chromatography (RP-18 column) from the crude methanolic extract. Ten phytocompounds, including 3-hydroxy-2{4-[(1E)-3-hydroxyprop-1-en-1-yl]-2methoxyphenoxy}propyl β-D-glucopyranoside (14), coniferin (15), Z-coniferin (16), syringin (eleutheroside B, 17), and dihydrosyringin (18), found in the extract of S. suaveolens leaves obtained from Vietnam. 26 In another study, from the aqueous fraction obtained in the methanol extract of the S. suaveolens leaves, Anh et al 14 isolated and elucidated the chemical structures of 6 compounds by using various chromatographic methods (RP-18, CC, highperformance liquid chromatography [HPLC], and thin layer chromatography [TLC]).…”

“…Gas chromatography-mass spectrometry analysis (GC-MS) identified 45 volatile constituents also determined in the EA extract of S. suaveolens leaves via a study by Anh et al 16 These volatile components, named 3-methyl-3-butenoic acid (29), diazoethane (30) 2-hexadecen-1-ol (67), n-hexadecanoic acid (68), sinapaldehyde (69), methyl linolelaidate (70), phytol (71), 2-palmitoyl glycerol (72), and diisooctyl phthalate (73). 16 Figure 2 shows the volatile compositions of S. suaveolens leaves.…”

Stixis scandensleaf extract-loading ZnO nanoparticles for porcine epidemic diarrhea virus (PEDV) treatment

“… 12 A similar plant, Stixis suaveolens Roxb, in the same family, has been studied for some of its chemical components. 13 From the Stixis suaveolens Roxb plant, Vietnamese scientists have developed a method to extract the compound Cappariloside A. 14 The compound which belongs to the alkaloid group has been shown to have the ability to fight many viruses, including influenza viruses H1N1 and H3N2, PIV3, and ADV.…”

Stixis suaveolens (Roxb.) Fruit Extract Deciphered Antidepressant and Antidiarrheal Effects via In vivo Approach

“…Recently, several phytochemical studies by Anh et al (2020) and Islam et al (2020), has been conducted with the fruit and other extracts of S. suaveolens to confirm its pharmacological activity. In this perspective, the present study aims to investigate the antidepressant and antidiarrheal properties of S. suaveolens through in vivo study.…”