Characterization of volatile compounds in flowers from four groups of sweet osmanthus (Osmanthus fragrans) cultivars

Abstract

S. 2013. Characterization of volatile compounds in flowers from four groups of sweet osmanthus (Osmanthus fragrans) cultivars. Can. J. Plant Sci. 93: 923Á931. Headspace-solid-phase microextraction (HS-SPME) and gas chromatography-mass spectroscopy (GC-MS) were used to characterize the volatiles in flowers of four cultivar groups of sweet osmanthus (Osmanthus fragrans Lour.), including Thunbergii, Latifolius, Aurantiacus and Semperflorens Groups. A total of 72 volatiles were identified. Volatile compounds and their relative contents varied among the four groups or cultivars within each group. Briefly, b-ionone, cis-linalool oxide (furan), translinalool oxide (furan) and linalool were the most common volatiles in tested cultivars, while (E)-2-hexenal, (Z)-3-hexen-1ol and hexanal were abundant in several cultivars. Principal component analysis showed that the Aurantiacus Group was rich in cis-and trans-linalool oxide (furan), whereas the Latifolius group had high levels of (E)-2-hexenal and (Z)-3-hexen-1-ol. Our results contribute to our understanding of the volatile composition and content in flowers from different osmanthus groups and will facilitate development of new osmanthus cultivars to meet requirements of the food and fragrance industries. , S. 2013. Caracte´risation des compose´s volatils chez les fleurs de quatre groupes de cultivars d'olivier odorant (Osmanthus fragrans). Can. J. Plant Sci. 93: 923Á931. Les auteurs ont recouru al a microextraction en phase solide-espace de teˆte (HS-SPME) et a`la chromatographie gazeuse-spectroscopie de masse (CG-SM) pour caracte´riser les compose´s volatils des fleurs de quatre groupes de cultivars d'olivier odorant (Osmanthus fragrans Lour.), a`savoir les groupes Thunbergii, Latifolius, Aurantiacus et Semperflorens. En tout, 72 compose´s ont e´teí dentifie´s. La nature et la concentration des compose´s volatils varient chez les quatre groupes et parmi les cultivars de chaque groupe. En re´sume´, le b-ionone, le cis-linalool oxyde (furane), le trans-linalool oxyde (furane) et le linalool figurent parmi les compose´s volatils les plus courants chez les varie´te´s examine´es, mais le (E)-2-hexenal, le (Z)-3-hexen-1-ol et l'hexanal abondent aussi chez plusieurs cultivars. L'analyse en composantes principales re´ve`le que le groupe Aurantiacus est riche en cis-et en trans-linalool oxyde (furane), tandis que les cultivars du groupe Latifolius se caracte´risent par une forte concentration de (E)-2-hexenal et de (Z)-3-hexen-1-ol. Ces re´sultats nous aideront a`mieux comprendre la nature et la concentration des compose´s volatils chez les fleurs de diffe´rents groupes d'osmanthes et faciliteront le de´veloppement de nouvelles varie´te´s qui re´pondront aux besoins des industries de l'alimentation et de la parfumerie.

Analysis of aroma-active compounds in three sweet osmanthus (Osmanthus fragrans) cultivars by GC-olfactometry and GC-MS

“…As Xin et al (2013) indicated that aroma characteristics in the same cultivar group had a high degree of similarity, three sweet osmanthus cultivars, HBYG, LYJG, and GCDG, each belonging to a different cultivar group and of major economic importance for flower production in central regions of China (Zhou et al, 2006), were chosen for comparing volatiles and aroma-active compounds in this study.…”

“…Collected flowers within each cultivar were mixed well and divided into 2 g samples. All samples were immediately put into airtight polyethylene bags, frozen and stored at −20 °C prior to analysis (Xin et al, 2013). Three biological replicates of each cultivar were used in each experiment.…”

“…The GC was fitted with an HP-5 column (30 m×0.25 mm×0.25 μm, Thermo Scientific, Bellefonte, PA, USA). The GC-MS conditions were modified from Xin et al (2013). The injector was maintained at 250 °C, with a transfer line temperature of 280 °C.…”

“…The injector and detector were maintained at 250 °C. The temperature program was modified according to Xin et al (2013) and set to 40 °C for 3 min, and then increased from 40 to 73 °C at 3 °C/min, held at 73 °C for 3 min, and finally raised to 220 °C at the rate of 5 °C/min and held for 1 min.…”

“…Solid-phase microextraction (SPME), a simple, rapid, sensitive, and solvent-free technique for flower aroma analysis, is considered largely to retain the natural aroma (Montero-Calderón et al, 2010). Using SPME, Xin et al (2013) found a high degree of similarity in aroma characteristics within the same cultivar group, and that the high relative contents of cis-and trans-linalool oxide (furan), trans-2-hexenal, and cis-3-hexen-1-ol might affect the aromas of sweet osmanthus. Cao et al (2009) suggested that the different relative contents of linalool, α-ionone, β-ionone, ocimene, and γ-decalactone in Albus, Luteus, and Aurantiacus cultivar groups led to variable aromas.…”