Characterization, pathogenicity and protective efficacy of a cell culture-derived porcine deltacoronavirus

Gao, Xiang; Zhao, Donghong; Zhou, Peng; Zhang, Liping; Li, Mingxia; Li, Weiyan; Zhang, Yongguang; Wang, Yonglu; Liu, Xinsheng

doi:10.1016/j.virusres.2020.197955

Cited by 17 publications

(7 citation statements)

References 30 publications

(56 reference statements)

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“…The inactivated vaccine induced PDCoV S-specific IgG and neutralizing antibody (NA) responses in immunized sows and piglets from immunized sows, and exhibited 87.1% protective efficacy in the piglets which were orally challenged with PDCoV strain NH (10 5 TCID 50/ piglet) at 5 days post-farrowing. Gao et al developed inactivated PDCoV vaccines by mixing binary ethyleneimine-inactivated cell culture-adapted CH/XJYN/2016 P30 with 206 adjuvant or Imject™ Alum Adjuvant ( 92 ). Gu et al found that short hairpin RNAs targeting M and N genes diminished PDCoV replication in ST cells and proposed that RNA interference (RNAi) is a promising new strategy against PDCoV infection ( 93 ).…”

Section: Current Anti-pdcov Strategiesmentioning

confidence: 99%

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Duan

2022

Front. Vet. Sci.

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The recent experience with SARS-COV-2 has raised our alarm about the cross-species transmissibility of coronaviruses and the emergence of new coronaviruses. Knowledge of this family of viruses needs to be constantly updated. Porcine deltacoronavirus (PDCoV), a newly emerging member of the genus Deltacoronavirus in the family Coronaviridae, is a swine enteropathogen that causes diarrhea in pigs and may lead to death in severe cases. Since PDCoV diarrhea first broke out in the United States in early 2014, PDCoV has been detected in many countries, such as South Korea, Japan and China. More importantly, PDCoV can also infect species other than pigs, and infections have even been reported in children, highlighting its potential for cross-species transmission. A thorough and systematic knowledge of the epidemiology and pathogenesis of PDCoV will not only help us control PDCoV infection, but also enable us to discover the common cellular pathways and key factors of coronaviruses. In this review, we summarize the current knowledge on the prevalence, pathogenicity and infection dynamics, pathogenesis and immune evasion strategies of PDCoV. The existing anti-PDCoV strategies and corresponding mechanisms of PDCoV infection are also introduced, aiming to provide suggestions for the prevention and treatment of PDCoV and zoonotic diseases.

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Section: Current Anti-pdcov Strategiesmentioning

confidence: 99%

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Duan

2022

Front. Vet. Sci.

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“…Also, avian infectious deltacoronaviruses include bulbul CoV-HKU11, thrush CoV-HKU12, munia CoV-HKU13, white-eye CoV-HKU16, sparrow CoV-HKU17, magpie robin CoV-HKU18, night heron CoV-HKU19, wigeon CoV-HKU20 and common moorhen CoV-HKU21 [30] , [61] . In addition, porcine deltacoronavirus infects pigs [62] , [63] .…”

Section: Animal and Human Infectious Coronavirusesmentioning

confidence: 99%

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

Seifi

Kamali

2021

Medicine in Drug Discovery

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Coronavirus disease-2019 has been one of the most challenging global epidemics of modern times with a large number of casualties combined with economic hardships across the world. Considering that there is still no definitive cure for the recent viral crisis, this article provides a review of nanomaterials with antiviral activity, with an emphasis on graphene and its derivatives, including graphene oxide, reduced graphene oxide and graphene quantum dots. The possible interactions between surfaces of such nanostructured materials with coronaviruses are discussed. The antiviral mechanisms of graphene materials can be related to events such as the inactivation of virus and/or the host cell receptor, electrostatic trapping and physico-chemical destruction of viral species. These effects can be enhanced by functionalization and/or decoration of carbons with species that enhances graphene-virus interactions. The low-cost and large-scale preparation of graphene materials with enhanced antiviral performances is an interesting research-direction to be explored.

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“…The prevention and control of PDCoV requires the implementation of effective vaccines. PDCoV is a new enteric pathogenic coronavirus that can cause severe diarrhea in piglets in recent years, but there are few studies on related vaccines at present [ 23 ]. However, no commercial vaccine is available, caused a serious problem in controlling the spread of PDCoV.…”

Section: Discussionmentioning

confidence: 99%

The recombinant pseudorabies virus expressing porcine deltacoronavirus spike protein is safe and effective for mice

Huang

et al. 2022

BMC Vet Res

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Background Porcine deltacoronavirus (PDCoV) is a new pathogenic porcine intestinal coronavirus, which has appeared in many countries since 2012. PDCoV disease caused acute diarrhea, vomiting, dehydration and death in piglets, resulted in significant economic loss to the pig industry. However, there is no commercially available vaccine for PDCoV. In this study, we constructed recombinant pseudorabies virus (rPRVXJ-delgE/gI/TK-S) expressing PDCoV spike (S) protein and evaluated its safety and immunogenicity in mice. Results The recombinant strain rPRVXJ-delgE/gI/TK-S obtained by CRISPR/Cas gE gene editing technology and homologous recombination technology has genetic stability in baby hamster syrian kidney-21 (BHK-21) cells and is safe to mice. After immunizing mice with rPRVXJ-delgE/gI/TK-S, the expression levels of IFN-γ and IL-4 in peripheral blood of mice were up-regulated, the proliferation of spleen-specific T lymphocytes and the percentage of CD4+ and CD8+ lymphocytes in mice spleen was increased. rPRVXJ-delgE/gI/TK-S showed good immunogenicity for mice. On the seventh day after booster immunity, PRV gB and PDCoV S specific antibodies were detected in mice, and the antibody level continued to increase, and the neutralizing antibody level reached the maximum at 28 days post- immunization (dpi). The recombinant strain can protect mice with 100% from the challenge of virulent strain (PRV XJ) and accelerate the detoxification of PDCoV in mice. Conclusion The recombinant rPRVXJ-delgE/gI/TK-S strain is safe and effective with strong immunogenicity and is expected to be a candidate vaccine against PDCoV and PRV.

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Characterization, pathogenicity and protective efficacy of a cell culture-derived porcine deltacoronavirus

Cited by 17 publications

References 30 publications

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

An Updated Review of Porcine Deltacoronavirus in Terms of Prevalence, Pathogenicity, Pathogenesis and Antiviral Strategy

Antiviral performance of graphene-based materials with emphasis on COVID-19: A review

The recombinant pseudorabies virus expressing porcine deltacoronavirus spike protein is safe and effective for mice

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