Characterization Study of Cytotoxicity of Green Synthesized ZnO Nanoparticles Loaded with Anti‐Cancer Doxorubicin Drug

Abstract

The green synthesized zinc oxide nanoparticle (ZnO) was filled with a commercially available anti-cancer drug, Doxorubicin (DoX). The loading of the drug into ZnO was validated using UV-Visible and Raman spectroscopy. X-ray diffraction data showed the wurtzite structure of the nanoparticles of ZnO. Scanning electron microphotography images detected the encapsulation of DoX drugs into ZnO nanoparticles. Fourier transformer infrared spectrum of DoX loaded ZnO showed a single band at 478 cm À 1 attributed to the ZnO vibrational band and the carbonyl group peak at 1578 cm À 1 , suggesting the loading of DoX into ZnO. In addition, in vitro cytotoxicity of DoX, ZnO and ZnO-DoX against HeLa cells was studied using the MTT colorimetric cell viability assay, which showed 99.4 % inhibition of ZnO-DoX cells at a concentration of 100 μg/mL. The findings thus provide compelling evidence for green biosynthesized ZnO, which could serve as a promising nanodrug carrier for the targeted drug delivery system.

WITHDRAWN: Cytotoxicity and wound healing efficacy of biosynthesis ZnO Nanoparticles Using Aspergillus niger against Multidrug Resistant Pseudomonas aeruginosa

“…The results suggest that the A. niger mediated ZnO NPs can effectively suppress the growth of studied cancer cell. Similar ndings have been reported by Padalia and Chanda [43] and Gomathi et al [44], whereby the capability of green ZnO-NPs to destroy cancer cells has been demonstrated. Furthermore, ZnO-NPs have been observed to disintegrate within the lysosome and donate Zn 2+ ions [45].…”

Comparison of Characteristics and Antimicrobial Activity of Synthesized Zinc Oxide And Magnetite Iron Oxide Nanoparticles Using Four Different Plant Extracts

“…Phytochemicals such as alcohols, phenols, amines, carboxylic acids, and others present in plant extracts have the capability to interact with the surfaces of zinc and iron, thereby aiding in the stabilization of nanoparticles. The broad peak within the 3400-3300 cm −1 range, a common occurrence in FTIR spectra, provides confirmation of the existence of intermolecular hydrogen bonding [24]. The absorption peaks at approximately ~1570 and ~1400 cm -1 in both spectra, known to come from plant extracts and help particle synthesis, are assigned to C=C stretching and C-C stretching vibrations, respectively.…”

Green synthesis and Biomedical Applications of Zinc Oxide Nanoparticles. Review

“…Zinc oxide-DOX cells were 99.4% inhibited at a dosage of 100 g/mL. Thus, the findings offer convincing support for green biosynthesized ZnO, which may work well as a potential nano-drug carrier for the targeted drug delivery system [96].…”