Characterizations of interaction between antimicrobial peptide (JCpep7) and living staphylococcus aureus used as pseudostationary phase in capillary electrochromatography

Taking Advantage of Electric Field Induced Bacterial Aggregation for the Study of Interactions between Bacteria and Macromolecules by Capillary Electrophoresis

“…Ding et al have also used ACE to determine the equilibrium constant between living Staphylococcus aureus and antimicrobial peptides. 29,30 The authors measured similar values as that found by Armstrong. 27 Nevertheless, it is worth noting that ACE does not allow determining quantitative information on the complex stoichiometry.…”

“…Different concentrations of B. infantis were added in the running buffer, and the measurement of the ligand electrophoretic mobility led to the determination of an apparent binding constant of 1.1 × 10 13 M –1 . Ding et al have also used ACE to determine the equilibrium constant between living Staphylococcus aureus and antimicrobial peptides. , The authors measured similar values as that found by Armstrong . Nevertheless, it is worth noting that ACE does not allow determining quantitative information on the complex stoichiometry.…”

“…For instance, CE has been used to determine viability of bacteria, microbial contamination in biological samples, or for quick sterility test . As the mechanism of action of antimicrobial agents commonly begins by the membrane adhesion, many works investigated the use of CE to study the interaction between drugs and microbial receptors − or with liposomes simulating membranes − or directly with the whole living cells. − In this latter case, Armstrong et al used affinity capillary electrophoresis (ACE) to study the interaction of Bifidobacterium infantis with Lucifer yellow and vancomycin . Different concentrations of B. infantis were added in the running buffer, and the measurement of the ligand electrophoretic mobility led to the determination of an apparent binding constant of 1.1 × 10 13 M –1 .…”