Characterizing Safflower Germplasm with AFLP Molecular Markers

Johnson, R. C.; Kisha, Theodore J.; Evans, Marc A.

doi:10.2135/cropsci2006.12.0757

Cited by 74 publications

(71 citation statements)

References 22 publications

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“…Recently, there is a raising interest in characterizing and exploiting its vast genetic diversity that can be further linked to desirable traits such as drought tolerance via molecular markers. Molecular markers have been used principally for the assessment of germplasm variability of local varieties, landraces, natural populations, and accessions in order to screen genetic diversity geographically (Amini et al 2008;Johnson et al 2007;Khan et al 2009;Sehgal and Raina 2005;Yang et al 2007). Since there is still inadequate genetic information, most of the markers did not require prior sequence information.…”

Section: Inferring Safflower Genetic Resource Diversitymentioning

confidence: 99%

“…Since there is still inadequate genetic information, most of the markers did not require prior sequence information. Hence, application of different molecular markers such as RAPD (Amini et al 2008;Mahasi et al 2009;Sehgal and Raina 2005;Vilatersana et al 2005), ISSR (Ash et al 2003;Golkar et al 2011;Panahi et al 2013;Sabzalian et al 2009;Yang et al 2007), AFLP (Johnson et al 2007;Sehgal and Raina 2005;Sehgal et al 2009), and SRAP (Mokhtari et al 2013;Peng et al 2008;Talebi et al 2012) has been successfully used in identifying genetic variety but had little to contribute for the identification of characteristics linked to drought tolerance. This may be the 'default' disadvantage of these marker systems since they 'scan' the genome arbitrary.…”

Section: Inferring Safflower Genetic Resource Diversitymentioning

confidence: 99%

“…As these markers are suitable for crops with insufficient genomic resource, these markers do not require any previous information and do genome scanning along with repetitive sequences. Many studies have reported safflower genetic diversity by using combination of phenotypic variation and molecular polymorphism (Amini et al 2013;Johnson et al 2007;Khan et al 2009;Yang et al 2007). However, development of genomic and molecular resources is limited in safflower as compared to other members of Asteraceae family, and commonly, SSR, RAPD, and AFLP markers are used for evaluation of landraces, germplasm, and genetic diversity and also to assess the outcrossing of safflower with weedy relatives (Sujatha et al 2008).…”

Section: Marker-assisted Selectionmentioning

confidence: 99%

See 2 more Smart Citations

Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

197

130

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Safflower is one of the oldest cultivated crops, usually grown at a small scale. Safflower is grown for flowers used for coloring, flavoring foods, dyes, medicinal properties, and livestock feed. Safflower is underutilized but gaining attention due to oil yield potential and the ability to grow under high temperatures, drought, and salinity. Salinity and drought have negative effects by disrupting the ionic and osmotic equilibrium of the plant cells. The stress signal is perceived by membranes then transduced in the cell to switch on the stress responsive genes. This review discusses on stress tolerance mechanisms in safflower. Strategies are proposed for enhancing drought and salt resistance in safflower.

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Section: Inferring Safflower Genetic Resource Diversitymentioning

confidence: 99%

Section: Inferring Safflower Genetic Resource Diversitymentioning

confidence: 99%

Section: Marker-assisted Selectionmentioning

confidence: 99%

See 1 more Smart Citation

Salt and drought stresses in safflower: a review

Hussain

Lyra

Farooq

et al. 2015

Agron. Sustain. Dev.

197

130

View full text Add to dashboard Cite

show abstract

“…The most useful molecular markers are inter-simple sequence repeats (ISSR), random amplified polymorphic DNA (RAPD) and amplified fragment length polymorphism (AFLP) (Kumari et al, 2013). These previous markers are used for crops with inadequate genomic resources because they do not need prior sequence information, and they have been used especially for assessing species genetic diversity (YazdiSamadi et al, 2001;Sehgal and Raina, 2005;Johnson et al, 2007). However, microsatellite markers are specific markers that require prior development for each species (De Vienne, 1998).…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Houmanat¹,

Charafi²,

Mazouz³

et al. 2016

IJAB

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Safflower (Carthamus tinctorius) is an oilseed crop with high quality vegetable seed oil. A set of 55 accessions from different geographical origins were introduced to constitute a breeding program germplasm. These materials were characterized by inter-simple sequence repeat (ISSR) as a simple method to reveal polymorphism among them. ISSR analysis was carried out with 13 primers. After agarose gels analysis, ISSR banding patterns were converted into binary data of presence-non presence and matrices were processed. Genetic distances between all pairwise combinations of the accessions were calculated using similarity of simple coefficient matching (SCM). Dendrogram of studied accessions was constructed using the UPGMA (unweighted pair group method arithmetic average) method. A high number of reproducible ISSR bands exhibited a high percentage of polymorphism (69.64%), indicating a high genetic diversity among the material studied. The lowest similarity coefficient was 0.462, observed between accessions 109 and 135, whilst the highest one was 0.962, observed between accessions 68 and 126, with an average of 0.726. Dendrogram similarity relationships revealed some clusters according to different genetic pools. The ISSR markers were very informative and exhibited a high level of polymorphism. The findings of this study would be useful for safflower breeding program in Morocco as well as in other countries of the world.

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“…In recent years a number of research groups have recognized the need for a molecular characterization of the safflower genome and the development of DNA markers (Maali-Amiri et al, 2001;Sehgal et al, 2009a;Johnson et al, 2007). One of the most important components required for an efficient system of molecular breeding is the identification and characterization of suitable genetic markers.…”

Section: Introductionmentioning

confidence: 99%

Genetic Variation of Safflower (Carthamus Tinctorius L.) and Related Species Revealed by ISSR Analysis

Bagmohammadi¹,

Pahlevani²,

Ahmadikhah³

et al. 2014

Plant Breeding and Seed Science

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Genetic diversity of eight genotypes of Carthamus tinctorius L., two populations of C. oxyacanthus, and one population of C. lanatus was investigated using inter-simple sequence repeat (ISSR) markers. All samples were uniquely distinguished by 10 ISSR primers with 144 bands which generated 100% polymorphism. Furthermore, the ISSR markers could separate three safflower species properly, that highlights the effectiveness of this marker system for phylogenetic studies. The most and least informative primers were ISSR9 (PIC=0.367) and ISSR2 (PIC=0.254), and some primers were more efficient in detecting polymorphism in one species than for the others. Unweighed pairgroup method with arithmetical averages (UPGMA) cluster analysis enabled construction of a dendrogram for estimating genetic distances among different populations. The result of cluster analysis suggested that cultivated and wild populations of C. oxyacanthus had close relationship with each other and far relationship with C. lanatus. The extreme genetic dissimilarity was observed between genotypes of C. tinctorius and C. lanatus populations. Based on the results, C. oxyacanthus could introduce favorable genes to cultivated safflower via inter-specific hybridization in breeding programs. Nei's gene diversity index, Shannon's index and percent of polymorphic loci showed that Isfahan ecotype of C. oxyacanthus had the highest variation at DNA level in relation to populations of other species. The ISSRs developed in this research along with those recently studied by other researchers will contribute to construct genetic map with a density sufficient for safflower molecular breeding.

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Characterizing Safflower Germplasm with AFLP Molecular Markers

Cited by 74 publications

References 22 publications

Salt and drought stresses in safflower: a review

Salt and drought stresses in safflower: a review

Genetic Diversity Analysis of Safflower (Carthamus tinctorius) Accessions from Different Geographic Origins using ISSR Markers

Genetic Variation of Safflower (Carthamus Tinctorius L.) and Related Species Revealed by ISSR Analysis

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