Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Schnabl, Jake; Litz, Mackenzie P.H.; Schneider, Charles L.; PenkoffLidbeck, Nadia; Bashiruddin, Sarah; Schwartz, Morgan; Alligood, Kristin; Devoto, Stephen H.; Barresi, Michael

doi:10.1002/dneu.22801

Cited by 4 publications

(3 citation statements)

References 116 publications

(318 reference statements)

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“…These midline glia express Slit1, which channels the forebrain axonal commissures by a permissive or positive mechanism ( Barresi et al, 2005 ). Moreover, Olig2+ glial cells of non-radial glial lineage in the telencephalon and of radial glial lineage in the diencephalon have been suggested to interact with developing commissures ( Schnabl et al, 2021 ).…”

Section: Axon Guidance By Astroglia: Insights From Zebrafishmentioning

confidence: 99%

Regulation of axon pathfinding by astroglia across genetic model organisms

Rapti

2023

Front. Cell. Neurosci.

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Glia and neurons are intimately associated throughout bilaterian nervous systems, and were early proposed to interact for patterning circuit assembly. The investigations of circuit formation progressed from early hypotheses of intermediate guideposts and a “glia blueprint”, to recent genetic and cell manipulations, and visualizations in vivo. An array of molecular factors are implicated in axon pathfinding but their number appears small relatively to circuit complexity. Comprehending this circuit complexity requires to identify unknown factors and dissect molecular topographies. Glia contribute to both aspects and certain studies provide molecular and functional insights into these contributions. Here, I survey glial roles in guiding axon navigation in vivo, emphasizing analogies, differences and open questions across major genetic models. I highlight studies pioneering the topic, and dissect recent findings that further advance our current molecular understanding. Circuits of the vertebrate forebrain, visual system and neural tube in zebrafish, mouse and chick, the Drosophila ventral cord and the C. elegans brain-like neuropil emerge as major contexts to study glial cell functions in axon navigation. I present astroglial cell types in these models, and their molecular and cellular interactions that drive axon guidance. I underline shared principles across models, conceptual or technical complications, and open questions that await investigation. Glia of the radial-astrocyte lineage, emerge as regulators of axon pathfinding, often employing common molecular factors across models. Yet this survey also highlights different involvements of glia in embryonic navigation or pioneer axon pathfinding, and unknowns in the molecular underpinnings of glial cell functions. Future cellular and molecular investigations should complete the comprehensive view of glial roles in circuit assembly.

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Section: Axon Guidance By Astroglia: Insights From Zebrafishmentioning

confidence: 99%

Regulation of axon pathfinding by astroglia across genetic model organisms

Rapti

2023

Front. Cell. Neurosci.

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“…These developmental instructions establish appropriate RGC axon pathfinding, synaptogenesis, and retinotopographic mapping, and many of these cues are conserved across mammalian and nonmammalian vertebrate visual systems [1,3,[11][12][13][14]. Efforts to date have largely focused on identifying axonogenesis and synaptogenesis mechanisms in primary visual processing areas, and how RGCs target other critical brain regions remains incompletely understood [15][16][17][18]).…”

Section: Introductionmentioning

confidence: 99%

Transcriptional control of visual neural circuit development by GS homeobox 1

Schmidt,

Placer,

Muhammad

et al. 2024

PLoS Genet

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As essential components of gene expression networks, transcription factors regulate neural circuit assembly. The homeobox transcription factor encoding gene, gs homeobox 1 (gsx1), is expressed in the developing visual system; however, no studies have examined its role in visual system formation. In zebrafish, retinal ganglion cell (RGC) axons that transmit visual information to the brain terminate in ten arborization fields (AFs) in the optic tectum (TeO), pretectum (Pr), and thalamus. Pretectal AFs (AF1-AF9) mediate distinct visual behaviors, yet we understand less about their development compared to AF10 in the TeO. Using gsx1 zebrafish mutants, immunohistochemistry, and transgenic lines, we observed that gsx1 is required for vesicular glutamate transporter, Tg(slc17a6b:DsRed), expression in the Pr, but not overall neuron number. gsx1 mutants have normal eye morphology, yet they exhibit impaired visual ability during prey capture. RGC axon volume in the gsx1 mutant Pr and TeO is reduced, and AF7 that is active during feeding is missing which is consistent with reduced hunting performance. Timed laser ablation of Tg(slc17a6b:DsRed)-positive cells reveals that they are necessary for AF7 formation. This work is the first to implicate gsx1 in establishing cell identity and functional neural circuits in the visual system.

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“…My lab started trying to answer those two questions by looking at the role of radial glia as the stem cell for the late-stage embryonic brain, and how those radial glia, or other related astroglial cells, influence nervous system wiring. My lab has looked at the Slit/Robo family of guidance cues and how they facilitate commissure development in the forebrain; we recently published a paper documenting a foundational architecture of what the embryonic forebrain looks like in zebrafish; more specifically, where cells are positioned relative to commissures (Schnabl et al, 2020). That's going to be a fantastic foundation for us moving forward to start to ask more mechanistic questions.…”

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confidence: 99%

An interview with Michael Barresi

Eve¹

2021

Development

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Michael Barresi is Professor of Biological Sciences at Smith College, Northampton, MA, USA, where he uses the zebrafish to understand central nervous system development. Michael is also Program Director of the ‘Student Scientists’ outreach project and has made significant contributions to teaching developmental biology, including being co-author and illustrator of the textbook Developmental Biology, producing developmental documentaries and starting the Online Developmental Teaching Forums. He was awarded the 2021 Viktor Hamburger Outstanding Educator Prize from the Society of Developmental Biology (SDB). We caught up with Michael over Teams to hear more about his career and love of learning.

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Characterizing the diverse cells that associate with the developing commissures of the zebrafish forebrain

Cited by 4 publications

References 116 publications

Regulation of axon pathfinding by astroglia across genetic model organisms

Regulation of axon pathfinding by astroglia across genetic model organisms

Transcriptional control of visual neural circuit development by GS homeobox 1

An interview with Michael Barresi

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