Characterizing the effects of Eugenol on neuronal ionic currents and hyperexcitability

Huang, Chin Wei; Chow, Julie Chi; Tsai, Jason Sheng Hong; Wu, Sheng‐Nan

doi:10.1007/s00213-011-2603-y

Cited by 45 publications

(33 citation statements)

References 60 publications

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“…1A; p < 0.001 vs. KA alone). These results are in agreement with previously published reports (Huang et al, 2012;Müller et al, 2006;Pourgholami et al, 1999), which suggest that EUG may exert anticonvulsant activity in a KA-induced model of TLE.…”

Section: Inhibitory Effects Of Eug On Ka-induced Gcd In the Dgsupporting

confidence: 95%

“…To confirm the beneficial effects of EUG against KA-induced seizures (Huang et al, 2012;Pourgholami et al, 1999), we evaluated whether EUG treatment delayed KA-induced seizure onset. Our results showed that 100 mg/kg EUG plus KA had no significant effect on seizure susceptibility (p = 0.074 vs. KA alone).…”

Section: Inhibitory Effects Of Eug On Ka-induced Gcd In the Dgmentioning

confidence: 99%

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Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Jeong¹,

Lee²,

Kim³

2015

Epilepsy Research

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Section: Inhibitory Effects Of Eug On Ka-induced Gcd In the Dgsupporting

confidence: 95%

Section: Inhibitory Effects Of Eug On Ka-induced Gcd In the Dgmentioning

confidence: 99%

Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Jeong¹,

Lee²,

Kim³

2015

Epilepsy Research

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“…and methylscopolamine (25 mg/kg; s.c.), before seizures were induced with pilocarpine injections (60 mg/kg; s.c.). All rats demonstrated behavioral characteristics during epileptic seizures that were similar to those reported previously [6], [27], [28]. Mouth and facial movements, head bobbing and nodding, scratching, masticatory automatisms, and exploratory behavior were observed within 15 to 20 min (stages 1–2).…”

Section: Methodssupporting

confidence: 80%

Pregabalin Attenuates Excitotoxicity in Diabetes

et al. 2013

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Diabetes can exacerbate seizures and worsen seizure-related brain damage. In the present study, we aimed to determine whether the standard antiepileptic drug pregabalin (PGB) protects against pilocarpine-induced seizures and excitotoxicity in diabetes. Adult male Sprague-Dawley rats were divided into either a streptozotocin (STZ)-induced diabetes group or a normal saline (NS) group. Both groups were further divided into subgroups that were treated intravenously with either PGB (15 mg/kg) or a vehicle; all groups were treated with subcutaneous pilocarpine (60 mg/kg) to induce seizures. To evaluate spontaneous recurrent seizures (SRS), PGB-pretreated rats were fed rat chow containing oral PGB (450 mg) for 28 consecutive days; vehicle-pretreated rats were fed regular chow. SRS frequency was monitored for 2 weeks from post-status epilepticus day 15. We evaluated both acute neuronal loss and chronic mossy fiber sprouting in the CA3 area. In addition, we performed patch clamp recordings to study evoked excitatory postsynaptic currents (eEPSCs) in hippocampal CA1 neurons for both vehicle-treated rats with SRS. Finally, we used an RNA interference knockdown method for Kir6.2 in a hippocampal cell line to evaluate PGB's effects in the presence of high-dose ATP. We found that compared to vehicle-treated rats, PGB-treated rats showed less severe acute seizure activity, reduced acute neuronal loss, and chronic mossy fiber sprouting. In the vehicle-treated STZ rats, eEPSC amplitude was significantly lower after PGB administration, but glibenclamide reversed this effect. The RNA interference study confirmed that PGB could counteract the ATP-sensitive potassium channel (KATP)-closing effect of high-dose ATP. By opening KATP, PGB protects against neuronal excitotoxicity, and is therefore a potential antiepileptogenic in diabetes. These findings might help develop a clinical algorithm for treating patients with epilepsy and comorbid metabolic disorders.

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“…The present study showed a negative chronotropic effect in Daphnia magna induced by CO alone at both concentrations, however more increased bradycardia was noted at the higher concentration of CO. The effects are similar to those observed by other authors who found that CO and other anaesthetics induce hypotension and bradycardia in fish and mammals (Lahlou et al, 2004;Mitchell et al, 2009;Huang et al, 2012). It is known that inhibition of heart rate caused by CO is associated with biological properties of eugenol which affects cardiac muscles acting as a Na + and Ca 2+ channel blocker, however its mechanisms of action on ionic channels both in vertebrates and invertebrates are not completely understood (Bekker and Krijgsman, 1951;Dyson et al, 1998;Chung et al, 2008).…”

Section: Heart Activitysupporting

confidence: 75%

Untitled

Bownik¹

2016

Turk. J. Fish. Aquat. Sci.

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Clove oil (CO) used as an anaesthetic in aquaculture causes toxic effects in fish and invertebrates since it has a narrow margin between effective and lethal concentrations. Ectoine (ECT) is an osmoprotectant produced by halophilic bacteria in response to stressful factors. It was hypothesized that ECT may attenuate physiological alterations induced by CO in aquatic animals. The aim of the present study was to determine if ECT induces protective effects in Daphnia magna subjected to COinduced anaesthesia. Daphnids were treated with 50 or 150 µL/L CO alone or in the combination with 10, 25 or 50 mg/L ECT. The results showed that both concentrations of CO alone induced anaesthesia, heart arrhythmia, decreased heart rate, increased diastole/systole ratio and duration of diastole. On the other hand, ECT attenuated alterations evoked by CO with the most significant effects in the combination of 50 µL/L CO+25 mg/L ECT. The animals showed a prolonged TtA and attenuated depression of thoracic limb activity, heart rate, arrhythmia, decreased diastole/systole ratio and duration of diastole.The results indicate that ECT may be a cardioprotective agent in anaesthesized crustaceans.

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Characterizing the effects of Eugenol on neuronal ionic currents and hyperexcitability

Abstract: The synergistic blocking effects of I (Na) and I(Na(NI)) contributes to the main mechanism through which EUG affects the firing of neuronal APs and modulate neuronal hyperexcitability such as pilocarpine-induced temporal lobe seizures.

Cited by 45 publications

References 60 publications

Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Effects of eugenol on granule cell dispersion in a mouse model of temporal lobe epilepsy

Pregabalin Attenuates Excitotoxicity in Diabetes

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