Characterizing the microstructures of biological tissues using Mueller matrix and transformed polarization parameters

Sun, Minghao; He, Honghui; Zeng, Nan; Du, Erdeng; Guo, Yige; Liu, Shaoxiong; Wu, Jian; He, Yonghong; Ma, Hui

doi:10.1364/boe.5.004223

Cited by 185 publications

(133 citation statements)

References 27 publications

Supporting

Mentioning

130

Contrasting

Order By: Relevance

“…A number of applications of Mueller polarimetric imaging are instead based on detecting unstained thin tissue cuts [22,131,144,[158][159][160][161][162] in a transmission geometry. Since Haematoxylin and Eosin (H&E) staining and other staining techniques require time and elaborate sample preparation by a well-trained and experienced pathologist for diagnosis, unstained Mueller microscopy may be used for rapid and low cost pathological inspection [159,160] and instant tissue characterization [136,163].…”

Section: The Biomedical Applications Of Mueller-polarimetric Imagingmentioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

View full text Add to dashboard Cite

Polarization is a fundamental property of light and a powerful sensing tool that has been applied to many areas. A Mueller matrix is a complete mathematical description of the polarization characteristics of objects that interact with light, and is known as a transfer function of Stokes vectors which characterise the state of polarization of light. Mueller polarimetric imaging measures Mueller matrices over a field of view and thus allows for visualising the polarization characteristics of the objects. It has emerged as a promising technique in recent years for tissue imaging, improving image contrast and providing a unique perspective to reveal additional information that cannot be resolved by other optical imaging modalities. This review introduces the basis of the Stokes-Mueller formulism, interpretation methods of Mueller matrices into fundamental polarization properties, polarization properties of biological tissues, and considerations in the construction of Mueller polarimetric imaging devices for surgical and diagnostic applications, including primary configurations, optimization procedures, calibration methods as well as the instrument polarization properties of several widelyused biomedical optical devices. The paper also reviews recent progress in Mueller polarimetric endoscopes and fibre Mueller polarimeters, followed by the future outlook in applying the technique to surgery and diagnostics. Tissue polarization properties convey morphological, micro-structural and compositional information of tissue with great potential for label free characterization of tissue pathological changes. Recent progress in tissue polarimetric imaging and polarization resolved endoscopy paved the way for translation of polarimetric imaging to surgery and tissue diagnosis.

show abstract

Section: The Biomedical Applications Of Mueller-polarimetric Imagingmentioning

confidence: 99%

Mueller polarimetric imaging for surgical and diagnostic applications: a review

2017

View full text Add to dashboard Cite

show abstract

“…Polarized light imaging/spectroscopy has been comprehensively investigated for tissue diagnosis [6][7][8][9][10][11][12][13][14][15][16][17]. Polarized light implementation offers several compelling advantages as follows: (i) surface and beneath-the-surface detection of biological tissue taken from the tissue depolarization properties [11,15]; (ii) tissue anisotropy analysis through the tissue diattentuation and retardance [13,14]; (3) enhanced tissue diagnosis through the combination of complementary depolarization, diattentuation and retardance of the tissues [9].…”

Section: Introductionmentioning

confidence: 99%

“…Polarized light implementation offers several compelling advantages as follows: (i) surface and beneath-the-surface detection of biological tissue taken from the tissue depolarization properties [11,15]; (ii) tissue anisotropy analysis through the tissue diattentuation and retardance [13,14]; (3) enhanced tissue diagnosis through the combination of complementary depolarization, diattentuation and retardance of the tissues [9]. Among the various polarized light imaging/spectroscopy techniques developed [6][7][8][9][10][11][12][13][14]18], MuellerMatrix polarimetry is capable of measuring the complete polarimetric transfer function [6][7][8][9], known as Mueller-matrix, of the bulk biological tissues which are optically inhomogeneous, birefringent, and absorbing media [19]. Currently, biomedical Mueller-Matrix polarimetry is mostly centered on the use of short visible wavelengths of illumination light that has a limited penetration depth and cannot detect lesions in deeper areas [8,9].…”

Section: Introductionmentioning

confidence: 99%

“…Among the various polarized light imaging/spectroscopy techniques developed [6][7][8][9][10][11][12][13][14]18], MuellerMatrix polarimetry is capable of measuring the complete polarimetric transfer function [6][7][8][9], known as Mueller-matrix, of the bulk biological tissues which are optically inhomogeneous, birefringent, and absorbing media [19]. Currently, biomedical Mueller-Matrix polarimetry is mostly centered on the use of short visible wavelengths of illumination light that has a limited penetration depth and cannot detect lesions in deeper areas [8,9]. The near-infrared (NIR) light, on the other hand, penetrates much deeper into the tissue, and is well suited for deep tissue diagnosis [12,[20][21][22].…”

Section: Introductionmentioning

confidence: 99%

“…The near-infrared (NIR) light, on the other hand, penetrates much deeper into the tissue, and is well suited for deep tissue diagnosis [12,[20][21][22]. Further, the reported Mueller-Matrix polarimetries only acquired either the images [7,8] or the optical spectra [9] of the biological tissues alone. In this work, we report on the development of a unique integrated Mueller-Matrix NIR imaging and pointwise Mueller-Matrix spectroscopy system for colonic tissue diagnosis and characterization.…”

Section: Introductionmentioning

confidence: 99%

See 2 more Smart Citations

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Wang

Zheng

Lin

et al. 2016

Biomed. Opt. Express

View full text Add to dashboard Cite

Abstract:We report the development and implementation of a unique integrated Mueller-matrix (MM) near-infrared (NIR) imaging and Muellermatrix point-wise diffuse reflectance (DR) spectroscopy technique for improving colonic cancer detection and diagnosis. Point-wise MM DR spectra can be acquired from any suspicious tissue areas indicated by MM imaging. A total of 30 paired colonic tissue specimens (normal vs. cancer) were measured using the integrated MM imaging and point-wise MM DR spectroscopy system. Polar decomposition algorithms are employed on the acquired images and spectra to derive three polarization metrics including depolarization, diattentuation and retardance for colonic tissue characterization. The decomposition results show that tissue depolarization and retardance are significantly decreased (p<0.001, paired 2-sided Student's t-test, n = 30); while the tissue diattentuation is significantly increased (p<0.001, paired 2-sided Student's t-test, n = 30) associated with colonic cancer. Further partial least squares discriminant analysis (PLS-DA) and leave-one tissue site-out, cross validation (LOSCV) show that the combination of the three polarization metrics provide the best diagnostic accuracy of 95.0% (sensitivity: 93.3%, and specificity: 96.7%) compared to either of the three polarization metrics (sensitivities of 93.3%, 83.3%, and 80.0%; and specificities of 90.0%, 96.7%, and 80.0%, respectively, for the depolarization, diattentuation and retardance metrics) for colonic cancer detection. This work suggests that the integrated MM NIR imaging and point-wise MM NIR diffuse reflectance spectroscopy has the potential to improve the early detection and diagnosis of malignant lesions in the colon. #258157Received 25

show abstract

Detection of non‐small cell lung cancer cells based on microfluidic polarization microscopic image analysis

Wang

Meng

et al. 2018

Electrophoresis

View full text Add to dashboard Cite

In early diagnosis of lung cancer, a polarization microscopy is a powerful tool to obtain the optical information of biological tissues. In this paper, a new microfluidic polarization imaging and analysis method was proposed for the detection and classification of cancer‐associated fibroblasts and the two kinds of non‐small cell lung cancer cells, A549 and H322. A polarizing microscopy system was constructed based on a commercial microscope to obtain 3*3 Mueller matrix of cells. Based on the Muller matrix decomposition algorithm and analysis in spatial domain and frequency domain, appropriate classification parameters were selected for the characterization of different polarization characteristics of cells. Finally, the logistic regression models based on machine learning were applied to determine optimal feature parameters and classify cells. This method integrated the morphological information of the cells, and the polarization characteristics of the cells in different polarization states. It is for the first time that the polarization microscopic image analysis method has been applied to the detection and classification of non‐small cell lung cancer cells. The results show that the presented microfluidic polarization microscopic image analysis method could classify cells effectively. Compared with the Muller matrix measurement and calculation methods, the method proposed in this paper was greatly simplified in both the acquisition of polarized images and the analysis and processing of polarized images.

show abstract

Characterizing the microstructures of biological tissues using Mueller matrix and transformed polarization parameters

Cited by 185 publications

References 27 publications

Mueller polarimetric imaging for surgical and diagnostic applications: a review

Mueller polarimetric imaging for surgical and diagnostic applications: a review

Integrated Mueller-matrix near-infrared imaging and point-wise spectroscopy improves colonic cancer detection

Detection of non‐small cell lung cancer cells based on microfluidic polarization microscopic image analysis

Contact Info

Product

Resources

About