Charge displacement by adhesion and spreading of a cell

Svetličić, Vesna; Ivošević, Nadica; Kovač, Solveg; Žutić, Vera

doi:10.1016/s0302-4598(00)00115-x

Cited by 31 publications

(42 citation statements)

References 16 publications

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“…The polarographic maximum of Hg(II) ions offers an alternative approach to measuring dissolved organic carbon of seawater (Hunter and Liss, 1981). However, adhesion of fluid SAP with hydrophobic properties is detected on the single particle level through spike-shaped signal (Žu-tić et al 1993;Svetličić et al, 2001;Ivošević DeNardis et al, 2007b). Collision of gel microparticle with the flexible three-dimensional network, thus possessing a hydrophilic character, is detected also on the single particle level through specific dip-shaped signal (Svetličić et al, 2006;Ivošević DeNardis et al, 2007a).…”

Section: Electrochemical Methodsmentioning

confidence: 99%

Application of surface analytical methods for hazardous situation in the Adriatic Sea: monitoring of organic matter dynamics and oil pollution

Pletikapić

DeNardis²

2017

Nat. Hazards Earth Syst. Sci.

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Abstract. Surface analytical methods are applied to examine the environmental status of seawaters. The present overview emphasizes advantages of combining surface analytical methods, applied to a hazardous situation in the Adriatic Sea, such as monitoring of the first aggregation phases of dissolved organic matter in order to potentially predict the massive mucilage formation and testing of oil spill cleanup. Such an approach, based on fast and direct characterization of organic matter and its high-resolution visualization, sets a continuous-scale description of organic matter from microto nanometre scales. Electrochemical method of chronoamperometry at the dropping mercury electrode meets the requirements for monitoring purposes due to the simple and fast analysis of a large number of natural seawater samples enabling simultaneous differentiation of organic constituents. In contrast, atomic force microscopy allows direct visualization of biotic and abiotic particles and provides an insight into structural organization of marine organic matter at micro-and nanometre scales. In the future, merging data at different spatial scales, taking into account experimental input on micrometre scale, observations on metre scale and modelling on kilometre scale, will be important for developing sophisticated technological platforms for knowledge transfer, reports and maps applicable for the marine environmental protection and management of the coastal area, especially for tourism, fishery and cruiser trafficking.

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Section: Electrochemical Methodsmentioning

confidence: 99%

Application of surface analytical methods for hazardous situation in the Adriatic Sea: monitoring of organic matter dynamics and oil pollution

Pletikapić

DeNardis²

2017

Nat. Hazards Earth Syst. Sci.

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“…Signals are defined by their amplitude (i.e. peak of adhesion signal) m I and duration d t as well as by displaced charge D q [10,11,27]. The displaced double-layer charge is obtained by integrating the area under the signal:…”

Section: Electrochemical Methodsmentioning

confidence: 99%

“…Interfacial behaviour of supported lipid layers, vesicles, model membranes and cells has been extensively studied at the mercury interface [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

“…We previously studied adhesion of the algal cell Dunaliella tertiolecta (D. tertiolecta) to the charged interface by recording the amperometric signal. The signal denotes establishment of intimate membrane contact at the charged mercury interface with subsequent cell spreading, followed by cell breakdown in a few milliseconds [10,11]. Analogously, the Scholz group studied adhesion of thrombocyte vesicles and mitochondria to the static mercury electrode [17,18].…”

Section: Introductionmentioning

confidence: 99%

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Cell adhesion and spreading at a charged interface: Insight into the mechanism using surface techniques and mathematical modelling

DeNardis

Pečar-Ilić

Ružić

et al. 2015

Electrochimica Acta

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Please cite this article as: Nadica Ivošević DeNardis, Jadranka Pečar Ilić, Ivica Ružić, Galja Pletikapić, Cell adhesion and spreading at a charged interface: Insight into the mechanism using surface techniques and mathematical modelling, Electrochimica Acta http://dx.(N. Ivošević DeNardis) 1 ISE member Highlights  Kinetics of adhesion and spreading of the algal cell at a charged interface is explored. Amperometric signals are analyzed using extended methodology and the reaction kinetics model.  The model reconstructs and quantifies individual states of the three-step adhesion process. Adhesion kinetics of the algal cell is slower than that of its plasma membrane vesicle. Slow spreading of organic film at the interface could be due to the attenuated effect of the potential. AbstractWe study the kinetics of adhesion and spreading of an algal cell and its plasma membrane vesicle at the charged interface. A simple system of an isolated plasma membrane vesicle without internal content has been developed and characterized by atomic force microscopy (AFM). We extend the methodology based on the reaction kinetics model and empirical fitting for the analysis of amperometric signals, and demonstrate its validity and pertinence in a wide range of surface charge densities. Adhesion kinetics of the algal cell is slower than that of its plasma membrane vesicle. Isolated plasma membrane contributes about one quarter to the cell contact area. The model reconstructs and quantifies individual states of the three-step adhesion process of the algal cell and makes it possible to associate them with various features of amperometric signal. At the time of current amplitude, the ruptured state predominates and the cell contact area is larger than its initial area as well as the contact area of the plasma membrane vesicle. These results suggest that a major structural disruption of the cell membrane, collapse of cytoskeleton and leakage of intracellular material could appear close to the time of current amplitude. Further, kinetics of the organic film spreading at the interface to its maximal extent is considered as the rate determining step, which could be a consequence of the attenuated effect of potential at the modified interface, stronger intermolecular interactions and reorganization of molecules in the film. Our findings offer an insight into the mechanism of algal cell adhesion and spreading at charged interfaces, relevant for electroporation based studies.

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“…Figure 2 shows the single-cell capture mechanism enabled by the potentiostatic control of the double-layer charge displacement [26] on the cell-capture electrodes in the cell chamber. The single-cell capture efficiency was optimized by setting the operating voltage at 1.0 V at a frequency of 10 kHz.…”

Section: Introductionmentioning

confidence: 99%

Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells

Yang

Cheing

et al. 2011

Interface Focus.

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A novel nanocapillary electrophoretic electrochemical (Nano-CEEC) chip has been developed to demonstrate the possibility of zeptomole-level detection of neurotransmitters released from single living cells. The chip integrates three subunits to collect and concentrate scarce neurotransmitters released from single PC-12 cells, including a pair of targeting electrodes for single cells captured by controlling the surface charge density; a dual-asymmetry electrokinetic flow device for sample collection, pre-concentration and separation in a nanochannel; and an online electrochemical detector for zeptomole-level sample detection. This Nano-CEEC chip integrates a polydimethylsiloxane microchannel for cell sampling and biomolecule separation and a silicon dioxide nanochannel for sample pre-concentration and amperometric detection. The cell-capture voltage ranges from 0.1 to 1.5 V with a frequency of 1-10 kHz for PC-12 cells, and the single cell-capture efficiency is optimized by varying the duration of the applied field. All of the processes, from cell sampling to neurotransmitter detection, can be completed within 15 min. Catecholamines, including dopamine and norepinephrine (noradrenaline) released from coupled single cells, have been successfully detected using the Nano-CEEC chip. A detection limit of 30-75 zeptomoles was achieved, which is close to the levels released by a single neuron in vitro.

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Charge displacement by adhesion and spreading of a cell

Cited by 31 publications

References 16 publications

Application of surface analytical methods for hazardous situation in the Adriatic Sea: monitoring of organic matter dynamics and oil pollution

Application of surface analytical methods for hazardous situation in the Adriatic Sea: monitoring of organic matter dynamics and oil pollution

Cell adhesion and spreading at a charged interface: Insight into the mechanism using surface techniques and mathematical modelling

Nanocapillary electrophoretic electrochemical chip: towards analysis of biochemicals released by single cells

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