Charge variants in serum and urine hCG

Sutton, Jaime M.

doi:10.1016/j.cccn.2003.12.006

Cited by 19 publications

(13 citation statements)

References 12 publications

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“…HCG is one of the most heavily glycosylated glycoprotein hormones, deriving around 30% of its molecular weight from eight oligosaccharide side chains, with 4 N‐linked oligosaccharides and 4 O‐linked oligosaccharides . This glycosylation makes hCG highly acidic, with a pI of around 3.5 . The acidity of this molecule impacts its circulating half‐life as acidic molecules are negatively charged and are therefore repelled by the negatively charged glomerular basement membrane .…”

Section: Isoforms and Sources Of Hcgmentioning

confidence: 99%

Hyperglycosylated hCG: a Unique Human Implantation and Invasion Factor

Evans

2015

American J Rep Immunol

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Section: Isoforms and Sources Of Hcgmentioning

confidence: 99%

Hyperglycosylated hCG: a Unique Human Implantation and Invasion Factor

Evans

2015

American J Rep Immunol

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“…These differences may arise from the fact that urinary hCG has been partially degraded during renal secretion, a process that depends in part on the sialylation status of the hormone (Liu et al 1989) and generates different populations of hCG in serum versus urine (Fein et al. 1980, Kovalevskaya et al 1999, Jeschke et al 2003, Sutton 2004. The binding of SNA to hCG from choriocarcinoma serum needs to be examined and may more closely correlate with that seen on hCG secreted immediately into the cell culture supernatant.…”

Section: Discussionmentioning

confidence: 99%

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Kelly

Birken

Puett

2007

Molecular and Cellular Endocrinology

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The ability to reliably detect aberrant glycosylation of human chorionic gonadotropin (hCG) may have profound implications for the diagnosis and monitoring of malignant gestational trophoblastic neoplasia, germ cell tumors, other malignancies, and pregnancy complications. To become a clinically useful assay, however, this discrimination of glycoforms should be possible on minimally treated biological specimens. Towards this end, we have developed a lectin-based sandwich-type immunoassay to compare the glycosylation patterns of hCG among urine specimens from patients presenting with a normal pregnancy, invasive mole, choriocarcinoma, and male germ cell tumors using carbohydrate-free antibody fragments as capture reagents and a panel of eight lectins, five recognizing neutral sugars and three recognizing sialic acid. There was no significant difference in the binding of any of the lectins to hCG in the urine of women over the gestational range of 6 -38 weeks. Three lectins, however, exhibited differential binding to urinary hCG derived from these normal pregnant controls and that from patients with malignant forms of gestational trophoblastic disease and male germ cell tumors. Galanthus nivalis agglutinin and Maackia amurensis lectin, which bind terminal mannose and α(2-3)sialic acid, respectively, preferentially bound pregnancyderived hCG, whereas the lectin, wheat germ agglutinin, which binds sialic acid and β(1-4)Nacetylglucosamine, exhibited decreased binding to pregnancy-derived hCG compared to that from patients with male germ cell tumors and malignant gestational trophoblastic neoplasia. The differential binding observed with these three promising lectins is most encouraging and warrants further examination. The experimental paradigm also holds promise for the development of comparable assays for other glycosylated tumor markers.

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“…At the 13 th week of gestation, the structure is changed, with a decreased degree of sialinization, and a shorter half-life of the hormone [6]. This decrease in half-life, added to a decrease in production rate, will cause a rapid decrease in the b-hCG levels in both serum and urine ( Figure 2).…”

Section: B-hcg During Pregnancy Pregnancy Testingmentioning

confidence: 93%

Consequences of inadvertent radioiodine treatment of Graves’ disease and thyroid cancer in undiagnosed pregnancy. Can we rely on routine pregnancy testing?

et al. 2008

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Doses to foetal thyroids were ablative (250-600 Gy). Total foetal dose in the Graves' patient was 100 mGy and compatible with survival, whereas a foetal dose of approximately 700 mGy together with induced hypothyroidism was fatal for the foetus of the cancer patient. Routine pregnancy tests may fail early and late in pregnancy. The possibility of pregnancy should be considered in all fertile women before therapy with radionuclides or cytostatic regimens, and a clinical investigation undertaken on wide indications with determination of serum beta-hCG, preferably together with an ultrasound examination.

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Charge variants in serum and urine hCG

Cited by 19 publications

References 12 publications

Hyperglycosylated hCG: a Unique Human Implantation and Invasion Factor

Hyperglycosylated hCG: a Unique Human Implantation and Invasion Factor

Determination of hyperglycosylated human chorionic gonadotropin produced by malignant gestational trophoblastic neoplasias and male germ cell tumors using a lectin-based immunoassay and surface plasmon resonance

Consequences of inadvertent radioiodine treatment of Graves’ disease and thyroid cancer in undiagnosed pregnancy. Can we rely on routine pregnancy testing?

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