Charge Variants of HLA-A and B Gene Products

Reekers, P.; Coates, D.; Doxiadis, Ilias; Ellis, Shirley A.; Klouda, P. T.; Hajek-Rosenmayr, A; Horst, A. van der; Little, Michelle; Madrigal, Alejandro; Wit, Tobias Rinke de

doi:10.1007/978-1-4612-3552-1_77

Cited by 5 publications

(4 citation statements)

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“…The paternal haplotype a (HLA-A3,B7,Cw7) is found in child Cl and haplotype b (HLA-A25,B37,C-) in children C2, C3, C4, acidic subtypes are B8 and B27.1. These observations are in good agreement with previous reports [6][7][8][14][15][16][17][18]. It is evident from this scheme that serologically homogeneous HLA class I antigens can be divided in two or more subtypes, requiring an adjustment of the nomen clature.…”

Section: Resultssupporting

confidence: 82%

“…The comparative analysis allowed the identification of typical banding patterns for most of the serologically defined HLA-A and HLA-B antigens. The HLA-related banding patterns were in good agreement with results reported by others [6][7][8][9][14][15][16][17][18], especially for the biochemically well established antigens like Al, A2.1 through A2.4, A23, A25, A26, A29, B7, B8, B13, B14, B18, B27.1 through B27.3, B37, B44.1, B44.2.…”

Section: Discussionsupporting

confidence: 79%

“…It is evident from this scheme that serologically homogeneous HLA class I antigens can be divided in two or more subtypes, requiring an adjustment of the nomen clature. In accordance with others [18] we labelled the biochemical subtypes of a given serologically defined HLA antigen with an arabic number starting with the most acidic subtype added after a period to the HLA designation. Furthermore, figure 3 demonstrates that some HLA-A and HLA-B gene products possess the same or similar isoelectric points.…”

Section: Resultsmentioning

confidence: 99%

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HLA Class I Biochemistry: Definition and Frequency Determination of Subtypes by One-Dimensional Isoelectric Focusing and Immunoblotting

Frenz¹,

Doxiadis²,

Vögeler³

et al. 1989

Vox Sanguinis

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Previous biochemical studies of HLA class I gene products were based on the use of radiolabelled lymphoblastoid cell lines and/or mitogen-stimulated peripheral blood lymphocytes (PBL). We present here a nonradioactive methodology for defining HLA class I polymorphism on a small number of cells, such as 5x10^6 lymphocytes, the vitality of which is not a requirement in contrast to radiolabelling techniques, where1-2x10^6 PBL are needed. We analyzed 54 serologically typed families with a total of 261 individuals and 214 haplotypes by one-dimensional isoelectric focusing and subsequent immunoblotting, using a polyclonal anti-class I heavy chain serum. The banding patterns segregated within families and correlated with the serological results. A substantial number of HLA-A and HLA-B products appeared biochemically heterogeneous allowing the definition of subtypes and the calculation of their frequencies.

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