2016
DOI: 10.1016/j.mrfmmm.2016.03.004
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Charged particle mutagenesis at low dose and fluence in mouse splenic T cells

Abstract: High-energy heavy charged particles (HZE ions) found in the deep space environment can significantly affect human health by inducing mutations and related cancers. To better understand the relation between HZE ion exposure and somatic mutation, we examined cell survival fraction, Aprt mutant frequencies, and the types of mutations detected for mouse splenic T cells exposed in vivo to graded doses of densely ionizing (48)Ti ions (1GeV/amu, LET=107 keV/μm), (56)Fe ions (1GeV/amu, LET=151 keV/μm) ions, or sparsel… Show more

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Cited by 3 publications
(3 citation statements)
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“…More recently, we confirmed the usefulness of these radiation signature mutations to demonstrate mutagenic effects with lower doses of Ti ions in the kidney epithelium (0.2 and 0.4 Gy)[14], and lower doses of Ti (0.2 and 0.4 Gy) and Fe ions (0.25 and 0.5 Gy) in splenic T cells [15]. While radiation signature mutations are also observed in spontaneous mutants from unexposed tissues, they are present at statistically reduced frequencies relative to exposed tissues.…”
Section: Discussionmentioning
confidence: 62%
See 1 more Smart Citation
“…More recently, we confirmed the usefulness of these radiation signature mutations to demonstrate mutagenic effects with lower doses of Ti ions in the kidney epithelium (0.2 and 0.4 Gy)[14], and lower doses of Ti (0.2 and 0.4 Gy) and Fe ions (0.25 and 0.5 Gy) in splenic T cells [15]. While radiation signature mutations are also observed in spontaneous mutants from unexposed tissues, they are present at statistically reduced frequencies relative to exposed tissues.…”
Section: Discussionmentioning
confidence: 62%
“…We use an in vivo system to study charged particle mutagenesis in the intact kidney epithelium in which the selectable autosomal mouse Aprt gene, located on chromosome 8, detects the full spectrum of mutations, from small intragenic changes to whole chromosome loss [1215, 19]. Charged particle exposure induces Aprt mutant cells, which persist for extended times in the kidney [12, 13] until isolated from the dispersed kidney via selection of the primary cells in culture.…”
Section: Introductionmentioning
confidence: 99%
“…And it is also a very good point to detect the mutation probability for the proliferated cells. Grygoryev et al explored the relationship between high‐energy heavy charged particles (HZE ions) exposure and somatic mutation, and found that densely ionizing HZE ions can induce characteristic mutations in splenic T cells at low fluency, and that at least a subset of radiation‐induced mutant cells is stably retained despite the apparent lack of increased mutant frequencies at the time of harvest …”
Section: Discussionmentioning
confidence: 99%