Chebulinic acid derived from triphala is a promising antitumour agent in human colorectal carcinoma cell lines

Wang, Min; Li, Yanru; Hu, Xianda

doi:10.1186/s12906-018-2412-5

Cited by 33 publications

(13 citation statements)

References 29 publications

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“…These observations led to the hypothesis that the pleiotropic functions of Triphala on regulating oxidative stress may depend on various conditions. Furthermore, as a multi-ingredient formulation, Triphala consists of three fruit herbal medicines and hundreds of components, the majority of which are polyphenols and their bioactive metabolites, such as gallic acid, quinic acid, teresautalic acid, chebulinic acid, corilagin, salicin, ethyl gallate, and methyl gallate [47][48][49][50]. Therefore, one point of further study should explore pharmacological mechanisms of the critical compounds with various concentrations in regulating antioxidant activities using different cell models.…”

Section: Discussionmentioning

confidence: 99%

[Retracted] Protective Effect of Triphala against Oxidative Stress‐Induced Neurotoxicity

Ning

Tsering

et al. 2021

BioMed Research International

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Background. Oxidative stress is implicated in the progression of many neurological diseases, which could be induced by various chemicals, such as hydrogen peroxide (H2O2) and acrylamide. Triphala is a well-recognized Ayurvedic medicine that possesses different therapeutic properties (e.g., antihistamine, antioxidant, anticancer, anti-inflammatory, antibacterial, and anticariogenic effects). However, little information is available regarding the neuroprotective effect of Triphala on oxidative stress. Materials and Methods. An in vitro H2O2-induced SH-SY5Y cell model and an in vivo acrylamide-induced zebrafish model were established. Cell viability, apoptosis, and proliferation were examined by MTT assay, ELISA, and flow cytometric analysis, respectively. The molecular mechanism underlying the antioxidant activity of Triphala against H2O2 was investigated dose dependently by Western blotting. The in vivo neuroprotective effect of Triphala on acrylamide-induced oxidative injury in Danio rerio was determined using immunofluorescence staining. Results. The results indicated that Triphala plays a neuroprotective role against H2O2 toxicity in inhibiting cell apoptosis and promoting cell proliferation. Furthermore, Triphala pretreatment suppressed the phosphorylation of the mitogen-activated protein kinase (MARK) signal pathway (p-Erk1/2, p-JNK1/2, and p-p38), whereas it restored the activities of antioxidant enzymes (superoxide dismutase 1 (SOD1) and catalase) in the H2O2-treated SH-SY5Y cells. Consistently, similar protective effects of Triphala were observed in declining neuroapoptosis and scavenging free radicals in the zebrafish central neural system, possessing a critical neuroprotective property against acrylamide-induced oxidative stress. Conclusion. In summary, Triphala is a promising neuroprotective agent against oxidative stress in SH-SY5Y cells and zebrafishes with significant antiapoptosis and antioxidant activities.

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Section: Discussionmentioning

confidence: 99%

[Retracted] Protective Effect of Triphala against Oxidative Stress‐Induced Neurotoxicity

Ning

Tsering

et al. 2021

BioMed Research International

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“…[58] Exerts anti-proliferative, apoptotic and anti-migratory effects in colon cancer cells. [59] Inhibits proliferation of gynecological cancers cell with IC50 values of 98.28-101.23 µg/mL against SKOV-3, HeLa, and HEC-1B cells. [60] Inhibits proliferation of HeLa, PANC-1, and MDA-MB-231 cells and suppresses the clonogenicity of HeLa cells.…”

Section: Therapeuticmentioning

confidence: 99%

Oxidative Stress and Cancer: Chemopreventive and Therapeutic Role of Triphala

Prasad

Srivastava

2020

Antioxidants

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Oxidative stress, caused by the overproduction of free radicals, leads to the development of many chronic diseases including cancer. Free radicals are known to damage cellular biomolecules like lipids, proteins, and DNA that results in activation of multiple signaling pathways, growth factors, transcription factors, kinases, inflammatory and cell cycle regulatory molecules. Antioxidants, which are classified as exogenous and endogenous, are responsible for the removal of free radicals and consequently the reduction in oxidative stress-mediated diseases. Diet and medicinal herbs are the major source of antioxidants. Triphala, which is a traditional Ayurvedic formulation that has been used for centuries, has been shown to have immense potential to boost antioxidant activity. It scavenges free radicals, restores antioxidant enzymes and non-enzyme levels, and decreases lipid peroxidation. In addition, Triphala is revered as a chemopreventive, chemotherapeutic, immunomodulatory, and radioprotective agent. Accumulated evidence has revealed that Triphala modulates multiple cell signaling pathways including, ERK, MAPK, NF-κB, Akt, c-Myc, VEGFR, mTOR, tubulin, p53, cyclin D1, anti-apoptotic and pro-apoptotic proteins. The present review focuses on the comprehensive appraisal of Triphala in oxidative stress and cancer.

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“…To evaluate the potential cytotoxicity on the normal cells, human embryonic kidney (HEK293) cells were used, and the cell viability was assessed by cell counting kit (CCK)-8 assays following the manufacturer’s protocol (Dojindo Inc., Kumamoto, Japan) ( Wang et al, 2018 ). Briefly, the cells were seeded in microtiter plates at a density of 1 × 10 4 cells per well for 24 h and cultured with increasing concentrations of AMPs ranging from 4 to 64 μmol⋅L –1 and cultured for 24 h. The CCK-8 solution was then added to each well, and the cells were incubated for an additional 3 h. The optical density (OD) was measured at 450 nm.…”

Section: Methodsmentioning

confidence: 99%

Identification and Characterization of Antimicrobial Peptides From Butterflies: An Integrated Bioinformatics and Experimental Study

Wang

Zhou

et al. 2021

Front. Microbiol.

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Butterflies represent one of the largest animal groups on Earth, yet antimicrobial peptides (AMPs) of this group are less studied in comparison with their moth counterparts. This study employed an integrated bioinformatics approach to survey natural AMPs from publicly available genomic datasets. Numerous AMPs, including cecropins, defensins, and moricins, were identified and subsequently used as templates for the design of a series of synthetic AMPs that mimicked the naturally occurring sequences. Despite differing biological effects among the various sequences, the synthetic AMPs exhibited potent antibacterial and antifungal activities in vitro and in vivo, without inducing hemolysis, which implied their therapeutic potential in infectious diseases. Electron and confocal fluorescence microscopies revealed that the AMPs induced distinct morphological and biophysical changes on microbial cell membranes and nuclei, suggesting that the antimicrobial effects were related to a mechanism of membrane penetration and nucleic acid binding by the peptides. In conclusion, this study not only offers insights into butterfly AMPs but also provides a practical strategy for high-throughput natural AMP discoveries that will have implications for future research in this area.

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Chebulinic acid derived from triphala is a promising antitumour agent in human colorectal carcinoma cell lines

Cited by 33 publications

References 29 publications

[Retracted] Protective Effect of Triphala against Oxidative Stress‐Induced Neurotoxicity

[Retracted] Protective Effect of Triphala against Oxidative Stress‐Induced Neurotoxicity

Oxidative Stress and Cancer: Chemopreventive and Therapeutic Role of Triphala

Identification and Characterization of Antimicrobial Peptides From Butterflies: An Integrated Bioinformatics and Experimental Study

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