Chelating properties of beer: Implications on calcium homeostasis in PE/CA-PJ15 cells

Granieri, Letizia; Pino, Alberto Marco Del; Mazzoni, Michela; Mancinelli, Loretta; Proietti, Primo; Perretti, Giuseppe; Palmerini, Carlo Alberto

doi:10.1016/j.jnim.2016.12.001

Cited by 4 publications

(3 citation statements)

References 32 publications

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“…Therefore, decomposition of O 2 · − apart from direct scavenging of • OH may additionally be responsible for inhibitory effect of studied phenolic acids on UPE from Fe 2+ -EGTA-H 2 O 2 system. These phenolic acids can also from complexes with divalent cations including Fe 2+ [ 33 , 34 , 35 ] which can decrease Fe 2+ reactivity with H 2 O 2 [ 33 , 34 ]. However, two-fold molar excess of EGTA compared to Fe 2+ ions in the reaction mixture seems to prevent formation of Fe 2+ -phenolic acid complexes.…”

Section: Discussionmentioning

confidence: 99%

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

et al. 2018

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Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe2+-EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid)–H2O2 system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380 to 630 nm). The UPE of 92.6 µmol/L Fe2+—185.2 µmol/L EGTA—2.6 mmol/L H2O2 reached 4319 ± 755 relative light units during 2 min measurement and was about seven times higher (p < 0.001) than the UPE of incomplete systems (Fe2+-H2O2, EGTA-H2O2) and medium alone. Substitution of Fe2+ with Cr2+, Co2+, Mn2+ or Cu2+ as well as of EGTA with EDTA (ethylenediaminetetraacetic acid) or citrate completely abolished UPE. Experiments with ROS scavengers revealed the dependence of UPE on hydroxyl radicals suggesting occurrence of oxidative attack and cleavage of the ether bond in EGTA backbone structure and formation of triplet excited carbonyl groups with subsequent light emission. Plant phenolics (ferulic, chlorogenic and caffec acids) at concentration 87 µmol/L and ascorbate at 0.46 mmol/L inhibited UPE by 90 ± 4%, 90 ± 5%, 97 ± 2% and 92 ± 1%, respectively. Quenching of UPE from Fe2+-EGTA-H2O2 system can be used for evaluation of antioxidant activity of phytochemicals.

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Section: Discussionmentioning

confidence: 99%

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

et al. 2018

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“…The findings above are generally supported by binding studies under nonphysiological contexts, which demonstrate that the affinity of specific binding sites toward Fe and Zn ions are dependent on structural features, element ion properties and pH (Andjelković et al., 2006; Dimitrić Marković, Marković, Brdarić, Pavelkić, & Jadranin, 2011; Sroka & Cisowski, 2003; Wei & Guo, 2014; Yang et al., 2014). On the other hand, the chelation properties of Ca have been less explored due to weaker affinity of the phenoxide group toward alkaline earth metals (Hider, Liu, & Khodr, 2001), although binding with phenolic acids has been reported (Granieri et al., 2017). Structures such as the catechol moiety and the chroman C‐ring ‐OH and ‐keto groups in flavonoids can provide bidentate coordination sites for elements (Andjelković et al., 2006).…”

Section: Ligands In Relation To Bioavailabilitymentioning

confidence: 99%

Opportunities for plant‐derived enhancers for iron, zinc, and calcium bioavailability: A review

Zhang

Stockmann

et al. 2020

Comp Rev Food Sci Food Safe

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Understanding of the mechanism of interactions between dietary elements, their salts, and complexing/binding ligands is vital to manage both deficiency and toxicity associated with essential element bioavailability. Numerous mineral ligands are found in both animal and plant foods and are known to exert bioactivity via element chelation resulting in modulation of antioxidant capacity or micobiome metabolism among other physiological outcomes. However, little is explored in the context of dietary mineral ligands and element bioavailability enhancement, particularly with respect to ligands from plant‐derived food sources. This review highlights a novel perspective to consider various plant macro/micronutrients as prospective bioavailability enhancing ligands of three essential elements (Fe, Zn, and Ca). We also delineate the molecular mechanisms of the ligand‐binding interactions underlying mineral bioaccessibility at the luminal level. We conclude that despite current understandings of some of the structure–activity relationships associated with strong mineral–ligand binding, the physiological links between ligands as element carriers and uptake at targeted sites throughout the gastrointestinal (GI) tract still require more research. The binding behavior of potential ligands in the human diet should be further elucidated and validated using pharmacokinetic approaches and GI models.

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“…Chlorogenic and caffeic acids effectively scavenged • OH in vitro and ferulic acid protected synaptosomes and neuronal cells from • OH‐induced damage . These phenolic acids were also reported to from complexes with divalent cations including Fe 2+ which can decrease Fe 2+ reactivity with H 2 O 2. Moreover, they inhibited • OH‐dependent UPE from the Fe 2+ –EGTA–H 2 O 2 system .…”

Section: Discussionmentioning

confidence: 99%

Light emission from the Fe²⁺–EDTA–ascorbic acid–H₂O₂ system strongly enhanced by plant phenolic acids

et al. 2019

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Oxidative reactions can result in the formation of electronically excited species that undergo radiative decay depending on electronic transition from the excited state to the ground state with subsequent ultra-weak photon emission (UPE). We investigated the UPE from the Fe 2+ -EDTA (ethylenediaminetetraacetic acid)-AA (ascorbic acid)-H 2 O 2 (hydrogen peroxide) system with a multitube luminometer (Peltier-cooled photon counter, spectral range 380-630 nm). The UPE, of 92.6 μmol/L Fe 2+ , 185.2 μmol/L EDTA, 472 μmol/L AA, 2.6 mmol/L H 2 O 2 , reached 1217 ± 118 relative light units during 2 min measurement and was about two times higher (P < 0.001) than the UPE of incomplete systems (Fe 2+ -AA-H 2 O 2 , Fe 2+ -EDTA-H 2 O 2 , AA-H 2 O 2 ) and medium alone. Substitution of Fe 2+ with Cr 2+ , Co 2+ , Mn 2+ or Cu 2+ as well as of EDTA with EGTA (ethylene glycol-bis(β-aminoethyl ether)-N,N,N′,N′-tetraacetic acid) or citrate powerfully inhibited UPE. Experiments with scavengers of reactive oxygen species (dimethyl sulfoxide, mannitol, sodium azide, superoxide dismutase) revealed the dependence of UPE only on hydroxyl radicals. Dimethyl sulfoxide at the concentration of 0.74 mmol/L inhibited UPE by 79 ± 4%. Plant phenolics (ferulic, chlorogenic and caffec acids) at the concentration of 870 μmol/L strongly enhanced UPE by 5-, 13.9-and 46.8-times (P < 0.001),respectively. It is suggested that augmentation of UPE from Fe 2+ -EDTA-AA-H 2 O 2 system can be applied for detection of these phytochemicals.

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Chelating properties of beer: Implications on calcium homeostasis in PE/CA-PJ15 cells

Cited by 4 publications

References 32 publications

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

Opportunities for plant‐derived enhancers for iron, zinc, and calcium bioavailability: A review

Light emission from the Fe²⁺–EDTA–ascorbic acid–H₂O₂ system strongly enhanced by plant phenolic acids

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Chelating properties of beer: Implications on calcium homeostasis in PE/CA-PJ15 cells

Cited by 4 publications

References 32 publications

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

Light Emission from the Fe2+-EGTA-H2O2 System: Possible Application for the Determination of Antioxidant Activity of Plant Phenolics

Opportunities for plant‐derived enhancers for iron, zinc, and calcium bioavailability: A review

Light emission from the Fe2+–EDTA–ascorbic acid–H2O2 system strongly enhanced by plant phenolic acids

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Product

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About

Light emission from the Fe²⁺–EDTA–ascorbic acid–H₂O₂ system strongly enhanced by plant phenolic acids