In this study, in order to examine the effect of fermentation on bioactive peptide (BAP) formation, samples were taken from fermented sucuks produced by the traditional method on days 0, 1, 3, 5 and 10, and peptide extractions were obtained. The extracted samples were enzymatically hydrolyzed using two different enzymes (pepsin and trypsin) and the hydrolysates were injected into HPLC and separated into peptide fractions through a column filled with Sephadex G-25 stationary phase. Lyophilized fractions were subjected to LC-MS/MS analysis to determine peptide profiles. According to LC-MS/MS mass spectrometry data of peptide fractions obtained from sucuk samples during the 10-day fermentation, a total of 10 different peptides were detected, including 6 different dipeptides (KD, LK, EL, KP, HL, IR) and 3 different tripeptides (GPP, GAA, RHA) with antioxidant activity and 1 tetrapeptide (CIRA) with antimicrobial activity. Antimicrobial and antioxidant activities of peptide fractions obtained from fermented sucuk samples subjected to enzymatic hydrolysis were investigated under laboratory conditions. Antioxidant activity determinations of peptide fractions were measured by the DPPH radical scavenging capacity method and it was determined that there was no statistical difference between the antioxidant activities of the fractions obtained on the 0th and 10th days of fermentation. It was determined that the fractions did not have sufficient antimicrobial effects against on Escherichia coli (ATCC 25922) and Listeria monocytogenes (ATCC 7644) bacteria, were moderately active on Salmonella typhimurium (ATCC 14028), and day 0 fractions were moderately active against Lactobacillus pentosus (ATCC 8041), also day 1, 3, 5 and 10 fractions were active.