Chemical analysis, antibacterial, and antioxidant activities of flavonoid‐rich extracts from four Moroccan propolis

This exploratory investigation aimed to determine the chemical composition and evaluate some biological properties, such as antioxidant, anti-inflammatory, antidiabetic, and antimicrobial activities, of Matricaria chamomilla L. essential oils (EOs). EOs of M. chamomilla were obtained by hydrodistillation and phytochemical screening was performed by gas chromatography–mass spectrophotometry (GC-MS). The antimicrobial activities were tested against different pathogenic strains of microorganisms by using disc diffusion assay, the minimum inhibitory concentration (MIC), and minimum bactericidal concentration (MBC) methods. The antidiabetic activity was performed in vitro using the enzyme inhibition test. The antioxidant activity of EOs was tested using the free radical scavenging ability (DPPH method), ferrous ion chelating (FIC) ability, and β-carotene bleaching assay. The anti-inflammatory effects were tested in vivo using the carrageenan-induced paw edema method and in vitro using the inhibition of the lipoxygenase test. The analysis of the phytochemical composition by GC-MS revealed that camphor (16.42%) was the major compound of EOs, followed by 3-carene (9.95%), β-myrcene (8.01%), and chamazulene (6.54%). MCEO, honey, and their mixture exhibited antioxidant activity against the DPPH assay (IC50 ranging from 533.89 ± 15.05 µg/mL to 1945.38 ± 12.71 µg/mL). The mixture exhibited the best radical scavenging activity, with an IC50 of 533.89 ± 15.05 µg/mL. As antidiabetic effect, EO presented the best values against α-glucosidase (265.57 ± 0.03 μg/mL) and α-amylase (121.44 ± 0.05 μg/mL). The EOs and honey mixture at a dose of 100 mg/kg exhibited a high anti-inflammatory effect, with 63.75% edema inhibition after 3 h. The impact of EOs on the studied species showed an excellent antimicrobial (Staphylococcus aureus ATCC 29213 (22.97 ± 0.16 mm)), antifungal (Aspergillus niger (18.13 ± 0.18 mm)) and anti-yeast (Candida albicans (21.07 ± 0.24 mm) effect against all the tested strains. The results obtained indicate that the EOs of M. chamomilla could be a potential drug target against diabetes, inflammation and microbial infections; however, further investigations to assess their bioactive molecules individually and in combination are greatly required.

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“…The BCB assay was carried out as previously reported by Belmehdi et al [20]. An emulsion of β-carotene, linoleic acid, and tween 40 was prepared.…”

Section: β- Carotene Bleaching Assay (Bcb)mentioning

confidence: 99%

Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture

et al. 2022

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“…Kaempferol and its glycosides process great antimicrobial activity [22]. Quercetin also, is a potent antibacterial molecule and has been shown to be effective against Staphylococcus aureus, as well as Staphylococcus epidermidis [23]. Likewise; apigenin was reported to have antibacterial activity against P. aeruginosa, and E. coli [24].…”

Section: Discussionmentioning

confidence: 99%

Phytochemical Profile and Antimicrobial Assessment of Abutilon fruticosum Guill. & Perr. Growing in Gebel Elba, Egypt

Gouda

Morsy²,

Youssef³

et al. 2022

Egypt. J. Chem.

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Abutilon fruticosum Guill. & Perr. (Family Malvaceae) is distributed throughout a number of arid and semi-arid regions and has been used in traditional and folk medicine. The current study was conducted to estimate the phytochemical profiling and antimicrobial activity of Abutilon fruticosum Guill. & Perr. growing in Gebel Elba, Egypt against some respiratory tract infections isolates (Gram-negative bacteria: Escherichia coli, Klebsiella pneumoniae, Pseudomonas aeruginosa, Enterobacter cloacae, and Acinetobacter baumannii; Gram-positive bacteria: Staphylococcus aureus, Streptococcus epidermidis, and Enterococcus faecalis; and yeast: Candida tropicalis). Phytoconstituents determination of Abutilon fruticosum was carried out using the high resolution ultra-performance liquid chromatography-quadrupole time-of-flight mass spectrometry (UPLC/qTOF-MS/ MS); which yielded the determination of thirty constituents including phenolic acids, alkaloids and the majority of flavonoids. Abutilon fruticosum extracts were tested for their in vitro antimicrobial activity using the disc diffusion method. Plant extracts exhibited potent antimicrobial activity against the tested isolates. Minimum inhibitory concentration (MIC) and minimum bactericidal concentration (MBC) for the most suitable successive fractions were determined. Butanol fraction showed the lowest MIC (25 mg/ ml) against all the tested organisms. While; ethyl acetate fraction exhibited the lowest MIC (12.5 mg/ ml) against Klebsiella pneumoniae. Abutilon fruticosum Guill. & Perr. extracts are enriched in phytoconstituents which are likely attributed to their potential antimicrobial activity.

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“…Pengujian MBC dilanjutkan dengan mengambil 10 µL dari sumur yang menunjukkan tidak adanya pertumbuhan patogen, kemudian ditumbuhkan pada media MHA dan diinkubasi dalam kurun waktu 24 jam pada suhu ruangan 37°C. Hasil uji MBC kemudian diamati dengan tidak adanya bakteri yang tumbuh di media MHA (Belmehdi et al, 2021).…”

Section: Gambar 1 Lokasi Pengambilan Sampel Mangrove DI Pantai Legon ...unclassified

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

Limbong,

Setyati,

Sedjati

et al. 2023

J. Kel. Trop.

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Mangrove association molds are known to produce secondary metabolite compounds that have antibacterial activity. The purpose of this study was to obtain anti-Staphylococcus epidermidis metabolites produced by the endophytic mold of mangrove Rhizophora apiculate. The screening process is carried out in exploratory descriptive research. The stages of this research are sampling, mold isolation and purification, and macroscopic characterization. Initial screening of antibacterial activity is carried out by the agar plug method. Isolates that produce antibacterial metabolites are molecularly identified. The isolate was then cultured with Potato Dextrose Agar (PDA) media. The producing antibacterial metabolites were extracted using two solvents of different polarities (ethyl acetate and methanol) by stratified maceration method. Antibacterial metabolites are then tested to determine the concentration of extracts required to inhibit (Minimum Inhibitory Concentration (MIC) and kill bacterial growth (Minimum Bactericidal Concentration / MBC). The results of this study showed that there is one species of mangrove endophytic mold Rhizophora apiculata that can produce antibacterial metabolites against S. epidermidis, namely Diaporthe sennae. These antibacterial metabolites are present in ethyl acetate extract. The resulting bioactivity based on the ability to inhibit bacterial growth can be seen from the MIC value of 2.5 mg / mL and the ability to kill bacteria with an MBC value of 5 mg / mL. The mold metabolite Diaporthe sennae could potentially be developed as an anti-Staphylococcus epidermidis Kapang asosiasi mangrove diketahui menghasilkan senyawa metabolit sekunder yang memiliki aktivitas antibakteri. Tujuan penelitian ini adalah untuk mendapatkan metabolit anti-Staphylococcus epidermidis yang dihasilkan oleh kapang endofit mangrove Rhizophora apiculate. Proses skrining dilakukan secara diskriptif eksploratif. Tahapan penelitian ini adalah pengambilan sampel, isolasi dan purifikasi kapang, serta karakterisasi makroskopis. Skrining awal aktivitas antibakteri dilakukan dengan metode agar plug. Isolat yang menghasilkan metabolit antibakteri diidentifikasi secara molekuler. Isolat tersebut selanjutnya dikultur dengan media Potato Dextrose Agar (PDA). Metabolit antibakteri yang dihasilkan diekstraksi menggunakan dua pelarut yang berbeda polaritas (etil asetat dan metanol) dengan metode maserasi bertingkat. Metabolit antibakteri kemudian diuji untuk menentukan konsentrasi ekstrak yang diperlukan untuk menghambat (Minimum Inhibitory Concentration /MIC) dan membunuh pertumbuhan bakteri (Minimum Bactericidal Concentration /MBC). Hasil penelitian ini menunjukkan bahwa terdapat satu spesies kapang endofit mangrove Rhizophora apiculata yang yang dapat menghasilkan metabolit antibakteri melawan S. epidermidis, yaitu Diaporthe sennae. Metabolit antibakteri tersebut terdapat dalam ekstrak etil asetat. Bioaktivitas yang dihasilkan berdasarkan kemampuan menghambat pertumbuhan bakteri terlihat dari nilai MIC sebesar 2,5 mg/mL dan kemampuan membunuh bakteri dengan nilai MBC sebesar 5 mg/mL. Metabolit kapang Diaporthe sennae berpotensi dikembangkan sebagai anti-Staphylococcus epidermidis

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Chemical analysis, antibacterial, and antioxidant activities of flavonoid‐rich extracts from four Moroccan propolis

Cited by 10 publications

References 38 publications

Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture

Determination of Chemical Compounds and Investigation of Biological Properties of Matricaria chamomilla Essential Oils, Honey, and Their Mixture

Phytochemical Profile and Antimicrobial Assessment of Abutilon fruticosum Guill. & Perr. Growing in Gebel Elba, Egypt

Potensi Metabolit Kapang Endofit Mangrove Rhizophora apiculata sebagai Anti-Staphylococcus epidermidis

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