Chemical Characteristics, Antimicrobial, and Cytotoxic Activities of the Essential Oil of Egyptian Cinnamomum glanduliferum Bark

Abstract

The essential oil isolated from the bark of Cinnamomum glanduliferum (Wall) Meissn grown in Egypt was screened for its composition as well as its biological activity for the first time. The chemical composition was analyzed by GC and GC/MS. The antimicrobial activity of the oil was assessed using agar-well diffusion method toward representatives for each of Gram-positive bacteria, Gram-negative bacteria, and fungi. The cytotoxic activity was checked using three human cancer cell lines. Twenty seven compounds were identified, representing 99.07% of the total detected components. The major constituents were eucalyptol (65.87%), terpinen-4-ol (7.57%), α-terpineol (7.39%). The essential oil possessed strong antimicrobial activities against Escherichia coli, with an activity index of one and minimum inhibitory concentration (MIC) equaling to 0.49 μg/ml. The essential oil possessed good antimicrobial activities against methicillin-resistant Staphylococcus aureus, Geotrichum candidum, Pseudomonas aeruginosa, Bacillus subtilis, Helicobacter pylori, Aspergillus fumigatus (MIC: 7.81, 1.95, 7.81, 0.98, 31.25, and 32.5 μg/ml, respectively). A considerable activity was reported against S. aureus and Mycobacterium tuberculosis (MIC; 32.5 and 31.25 μg/ml, respectively). The extracted oil was cytotoxic to colon (HCT-116), liver (HepG2), and breast (MCF-7) carcinoma cell lines with IC of 9.1, 42.4, and 57.3 μg/ml, respectively. These results revealed that Egyptian Cinnamomum glanduliferum bark oil exerts antimicrobial and cytotoxic activities mainly due to eucalyptol and other major compounds.

Chemical Composition, Phytotoxic, Antimicrobial and Insecticidal Activity of the Essential Oils of Dracocephalum integrifolium

“…Antimicrobial activity of essential oils could be attributed to the high content of certain components; for instance, the high antimicrobial activity of Thymus pulegioides oil was caused by one the its abundant constituents, thymol [58,59]. Essential oils rich in eucalyptol are reported to possess antimicrobial activity; eucalyptol was found to exhibit inhibitory activity against various microorganisms such as E. coli , S. aureus , B. cereus , P. aeruginosa , etc.…”

Anti-inflammatory and gastroprotective potential of leaf essential oil of Cinnamomum glanduliferum in ethanol-induced rat experimental gastritis

“…On the other hand, indomethacin treated animals in group II resulted in mean PGE2 concentration of 2.1 ± 0.3 pg/mL in inflammatory exudates compared to group I of carrageenan challenge that resulted in 4.4 ± 0.5 (Figure 2(B)). Animals receiving volatile oil showed significant reduction of the PGE2 concentration by 1.5, 1.8 and 2.5 folds (to reach 2.95 ± 0.2, (Taha & Eldahshan 2017). 2.45 ± 0.15 and 1.75 ± 0.015 pg/mL) for the doses of 250, 500 and 1000 mg/kg, respectively).…”

“…In a previous study, C. glanduliferum bark volatile oil in Egypt was subjected to GC-MS analysis (Taha & Eldahshan 2017) but leaf and stem have not been studied for yet. Thus our aim in this study was phyto-investigation of its volatile oil (leaves and green branches) as well as the anti-inflammatory and gastroprotective effects of the volatile oil in leaves.…”

Curcuma raktakanda Induces Apoptosis and Suppresses Migration in Cancer Cells: Role of Reactive Oxygen Species

“…Similarly, eicosane (macrolide) produces cytotoxicity both in vitro and in nude mice bearing human ovarian carcinoma cells [37]. Eucalyptol and elemene, identified in the hexane and ethylacetate extracts, are also known to exhibit anti-cancer activities [38,39,40,41]. Whether the components of the extract interact in an additive, synergistic, or antagonistic manner remains to be explored.…”