Chemical characterization and antioxidant activity of three medicinal Apiaceae species

Abstract

a b s t r a c tCentella asiatica ('Pegaga' = Malaysia) is well known plant that has been used as one of the ingredients in pharmaceutical and cosmetic industries. Using liquid chromatography with diode array and electrospray ionization/mass spectrometry (LC-DAD-ESI/MS) analysis, chemical profiling of three different Pegaga extracts (C. asiatica, H. bonariensis and H. sibthorpioides) revealed variations in their metabolite profile. Our findings showed that triterpenes of C. asiatica were characterized by the ursane-type triterpenes (madecassoside, asiaticoside, madecassic acid, and asiatic acid), while the two Hydrocotyle species consisted of oleanane-type triterpenes (barrigenol derivatives). Other variations are due to the difference in phenolic and flavonoid constituents. The three Pegaga extracts were also evaluated for their total phenolic content (TPC), 1,1-diphenyl-2-picrylhydrazyl (DPPH) free radicals, and xanthine oxidase inhibition (XOI) activities. The results showed that C. asiatica has the most potent antioxidant activity (TPC = 72.09 mg/100 g DW; DPPH = 72.99 g/ml; XOI = 87.68 g/ml) as compared to H. bonariensis (TPC = 28.55 mg/100 g DW; DPPH = 22.43 g/ml; XOI = 32.23 g/ml) and H. sibthorpioides (TPC = 56.23 mg/100 g DW; DPPH = 37.86 g/ml; XOI = 14.73. g/ml).

Sea fennel (Crithmum maritimum L.): phytochemical profile, antioxidative, cholinesterase inhibitory and vasodilatory activity

“…The presented results were in accordance with those previously described by Meot-Duros and Magné (2009) who reported that sea fennel was one of the most chlorogenic acid-rich Apiaceae plants. Similar observations could be made regarding the results on phenolics and chlorogenic acid content in other Apiaceae species (Pandey et al 2012;Maulidiani et al 2014).…”

Ethnobotanical Survey of the Medicinal Plants used in the Southern Mediterranean. Case Study: The Region of Bissa (Northeastern Dahra Mountains, Algeria)

“…According to Güzel et al 8 the popularity of Lamiaceae and Asteraceae may be due to their aromatic characteristics and to their wide geographical spread. Several studies have confirmed the benefits of these families, according to Miliauskas et al 43 Khled Khoudja et al 44 Maulidiani et al 45 their importance lies in their high phenolic and flavonoid contents, compounds responsible for the antioxidant activity. Finally, in addition to their therapeutic role, the herbaceous families provide a favorable environment for the germination of the shrub layer seeds and the growth of young seedlings.…”

Phytochemical and biological features of Phyllanthus niruri and Phyllanthus urinaria harvested at different growth stages revealed by 1 H NMR-based metabolomics

“…The determination of the total phenolic content of the extracts was performed using Folin-Ciocalteu reagent as described by Maulidiani et al (2014). A 10 l alliquot of all the extracts (500 mg/ml) in triplicate was placed in a 96-well microplate followed by the addition of 25 l of freshly prepared Folin-Ciocalteu reagent (10-fold dilution).…”

“…The radical scavenging activity was determined according to Maulidiani et al (2014) with slight modification. Briefly, 150 l of DPPH (0.3 mM in ethanol) was added to 50 l of test extract in a 96-well microtiter plate.…”