Chemical Characterization and Larvicidal Activity of Essential Oil from Aniba duckei Kostermans against Aedes aegypti

Abstract

ABSTRACT-Aedes aegypti mosquito is the major vector of zika, chikungunya, and dengue fever. These diseases incidence has been growing rapidly in many points of the globe in the past few years. And because there's no vaccine for them yet, the best way to fight those diseases is to attack their vector, specially by eliminating potential sites for its oviposition and larvae growth. Nowadays, organophosphorus insecticides are used in increasing doses, which targets Aedes aegypti resistant populations. Aniba duckei Kostermans, which is known as rosewood and belongs to the Lauraceae family, is a species with trees up to 30 meters tall and 1 meter in trunk diameter. It's essential oil is used in perfumery due to its high content of linalool. This research identified the components of essential oil from Aniba duckei Kostermans thin branches and leaves and then applied it as larvicide against Aedes aegypti, and its effects were measured by calculation of concentration at which half larvae die (LC 50 ). Average yield found for oil by plant was 1.93% by mass. The major component in rosewood essential oil is linalool, whose concentration was found 89.34% by mass. LC 50 for the essential oil was 250.61 (±2.20) µg mL -1 , for l-linalool, 279.89 (±2.12) µg mL -1 , and for dl-linalool was 346.73 (±2.14) µg mL -1 .

Chemical Diversity and Therapeutic Effects of Essential Oils of Aniba Species from the Amazon: A Review

“…Linalool varied from 71.76% to 93.60%, followed by β-selinene (0.64–6.41%), α-terpineol (1.11–5.6%), spathulenol (0.34–4.0%), caryophyllene oxide (2.0–3.2%), and cis -linalool oxide (1.6–3.03%), in smaller proportions ( Table 1 ). The Amazonas collection sites were Itacoatiara (Adu6-LT), Presidente Figueiredo (Aro6-L), Novo Airão (Aro8-L, Aro9-L, Aro10-L and Aro11-L), Maués (Aro25-LT, Aro26-LT, Aro27-LT, Aro29-L, Aro29-Br and Aro29-LBr), Novo Aripuanã (Aro30-L, Aro30-Br and Aro30-LBr), Adolpho Ducke Forest Reserve in Manaus (Adu3-L, AduBr-4 and Aro66-LT) and Aro69-L (collection site not indicated) [ 25 , 29 , 30 , 77 , 78 , 79 , 80 , 81 , 82 ].…”

“…Aniba rosaeodora oil was more efficient against Aeromonas caviae and Enterococcus faecalis than the standard linalool. Linalool exhibited more significant activity against Klebsiella pneumonia and Providencia stuartii compared to the oil, while the oil and linalool presented the same activity against Aeromonas hydrophila [ 81 ]. The oil from stems of A. rosaeodora was also tested against E. coli and S. aureus and presented MIC of 200 and 150 μg/mL, respectively.…”

“…The A. rosaeodora leaves and thin branches oil, collected in Adolpho Ducke Forest Reserve (AM, Brazil), containing linalool (93.60%), α-terpinolene (3.37%), and cis -linalool oxide (3.03%), and the linalool standard were tested by the ABTS method. The effective concentration necessary to scavenge 50% of the ABTS (EC 50 ) was 15.46 μg/mL for A. rosaeodora oil and 6.78 μg/mL for linalool isolated [ 81 ].…”

“…The A. rosaeodora leaf and thin branch oil containing linalool (93.60%), α-terpinolene (3.37%), and cis -linalool oxide (3.03%), linalool standard (1000–7.8 μg/mL) and the positive control benznidazole (200–0.78 μg/mL) were evaluated by MTT method on cell viability of peritoneal macrophages from Balb/C mice. The oil and linalool did not exhibit cell toxicity at the highest concentration analyzed (CC 50 > 1000 μg/mL) [ 81 ].…”

“…The best larvicidal activity was detected for the oil (LC 50 250.61 μg/mL), while (3 R )-(–)-linalool and racemic (±)-linalool showed LC 50 values of 279.89 μg/mL and 346 μg/mL, respectively. The organophosphate Temephos (100 ppm) and mineral water containing 0.04% Tween (20 mL) were used as the positive and negative control, respectively [ 81 ].…”