Chemical composition and acaricidal activity of essential oils from Peperomia pellucida Kunth. against Tetranychus urticae

Abstract

Composição química e atividade acaricida dos óleos essenciais de Peperomia pellucida Kunth. contra Tetranychus urticaeResumo: Os óleos essenciais obtidos por hidrodestilação das folhas e caules de Peperomia pellucida coletadas de um fragmento de Mata Atlântica no estado da Bahia, Brasil, foram analisados utilizando cromatografia gasosa (CG) e cromatografia gasosa acoplada à espectrometria de massas (CG-EM). Independentemente da parte da planta, sesquiterpenos foram a classe de compostos mais abundantes nos óleos, seguido de fenilpropanoides. Os principais constituintes do óleo das folhas foram γ-gurjuneno (11,34%±0,01), 1,10-di-epi-cubenol (11,27%±0,02), (E)-cariofileno (8,71%±0,02) e dilapiol (8,50%±0,03), enquanto que carotol (9,77%±0,01), dilapiol (9,18%±0,01), trans-β-guaieno (9,05%±0,02) e (E)-cariofileno (8,53%±0,02) foram os principais constituintes do óleo do caule. A atividade dos óleos contra Tetranychus urticae foi avaliada por 24 h. O óleo do caule (CL 50 = 1 x 10 -2 µL/L de ar) foi quatro vezes mais tóxico do que o óleo de folha (CL 50 = 4 x 10 -2 µL/L de ar), mas 2,5 vezes menos ativo do que o eugenol, o qual foi utilizado como o controle positivo. Este estudo revelou diferentes perfis químicos para os óleos da folha e caule e reporta pela primeira vez a ação acaricida do óleo essencial de P. pellucida contra uma importante praga agrícola. Palavras-chave:Peperomia pellucida; Tetranychus urticae; sesquiterpeno; fenilpropanoide; γ-gurjunene; carotol. AbstractEssential oils obtained through hydrodistillation of the leaves and stems of Peperomia pellucida collected from a fragment of the Atlantic Forest in the state of Bahia, Brazil, were analyzed using gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS). Independently of the part of the plant, sesquiterpenes were the most abundant class of compounds in the oils, followed by phe ylpropa oids. The ai o stitue ts i the leaf oil were γ-gurjunene (11.34%±0.01), 1,10-di-epicubenol (11.27%±0.02), (E)-caryophyllene (8.71%±0.02) and dillapiole (8.50%±0.03). The major constituents of the stem oil were carotol (9.77%±0.01), dillapiole (9.18%±0.01), trans-β-guaiene (9.05%±0.02) and (E)-caryophyllene (8.53%±0.02). The activity of the oils against Tetranychus urticae was evaluated for 24 h. The stem oil (LC 50 = 1 x 10 -2 µL/L of air) was fourfold more toxic than the leaf oil (LC 50 = 4 x 10 -2 µL/L of air), but 2.5-fold less active than eugenol, which was used as the positive control. This study revealed different chemical profiles for the leaf and stem oils and is the first report of the acaricidal activity of P. pellucida essential oil against an important agricultural pest.

Ethnobotanical Uses, Phytochemistry and Pharmacological Activities of Peperomia Pellucida (L.) Kunth (Piperaceae)-a Review

“…In a study, de Oliveira et al [62] evaluated the activity of the essential oils from leaf and stem against Tetranychus urticae. It was observed that the stem oil was fourfold more toxic than the leaf oil however the activity of essential oils was lesser than eugenol, the positive control.…”

The potential of several compounds in Peperomia pellucida as a solution to rifampicin resistance in tuberculosis disease

“…Peperomia pellucida Kunth. is a shrub-type plant with succulent leaves that have a distinctive aroma and grow in moist soil and wet rock crevices (De Oliveira et al, 2017).…”