Chemical composition and antimicrobial activity of Hedyosmum brasiliense Miq., Chloranthaceae, essential oil

Abstract

RESUMO:Óleo essencial de Hedyosmum brasiliense Miq., Chloranthaceae: composição e atividade antimicrobiana. Hedyosmum brasiliense Miq., Chloranthaceae, é uma espécie endêmica no Brasil, conhecida como "cidrão". Embora H. brasiliense seja utilizada como calmante na medicina popular, não foi ainda caracterizada quanto aos constituintes químicos. Este trabalho apresenta a composição do óleo essencial, obtido pela destilação de folhas frescas e de amostra de óleo essencial armazenada por três meses, analisadas por GC-FID e CG-MS. Os efeitos inibitórios do óleo essencial foram testados pelo método da diluição em agar, contra seis espécies de bactérias (Bacillus subtilis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus e S. saprophyticus). Adicionalmente, seis espécies de fungos (Candida albicans, C. parapsilosis, Microsporum canis, M. gypseum, Trichophyton rubrum e T. mentagrophytes) foram incluídas. Dentre os principais constituintes, α-terpineol (10,2%), curzereno (8,9%), pinocarvona (8,4%) e β-tujeno (7,1%) foram os encontrados em maior quantidade. O óleo essencial apresentou baixa atividade contra microorganismos Gram-negativos. Entretanto, a atividade é extraordinária contra bactérias Gram-positivas e fungos, com valores de MIC entre 0,125 a 2,5% (v/v). Unitermos:Hedyosmum brasiliense, Chloranthaceae, óleo essencial, atividade antimicrobiana. ABSTRACT:Hedyosmum brasiliense Miq., Chloranthaceae, is an endemic species of Brazil, locally known as "cidrão". Although H. brasiliense is popularly used as sedative, chemical constituents of this species remains uncharacterized. This work presents the essential oil composition, obtained by the distillation of fresh leaves and a sample stored for three months, analysed by GC-FID and GC-MS. The inhibitory effects of the essential oil were tested by the agar dilution method against six bacterial species (Bacillus subtilis, Escherichia coli, Proteus mirabilis, Pseudomonas aeruginosa, Staphylococcus aureus and S. saprophyticus). In addition, six fungal species (Candida albicans, C. parapsilosis, Microsporum canis, M. gypseum, Trichophyton rubrum and T. mentagrophytes) were included. Among the determined constituents, α-terpineol (10.2%), curzerene (8.9%), pinocarvone (8.4%) and β-thujene (7.1%) were found as the main components. The essential oil has low activity only against Gram-negative microorganisms. However, it is a remarkable active against Grampositive bacteria and fungi with MIC values ranging from 0.125 to 2.5% (v/v).

The Evaluation of the Antibacterial and Antioxidant Activity of Tarragon (Artemisia dracunculus L.) Essential Oil and Its Chemical Composition

“…The antibacterial activity of the essential oil is dependent upon its major compounds. However some researchers reported higher antibacterial activity of the whole essence than major compounds blended in essence due to antibacterial properties of minor compounds in essential oil (16,22). Based on the results of this study, the essence of Tarragon had biological properties as anti-oxidative and antibacterial activities.…”

Phytochemical Investigation of Male and Female Hedyosmum scabrum (Ruiz & Pav.) Solms Leaves from Ecuador

“…[ ][ ] On the other hand, five new guaiane‐type sesquiterpenoids were isolated from the aerial parts of H. orientale and a new flavonoid with a significant analgesic activity was isolated from H. bonpiandianum . The essential oils from leaves and fruits of H. mexicanum , H. bonplandianum , H. costaricensis from Costa Rica , H. colombianum from Colombia, and from leaves of H. brasiliense from Brazil were studied. The essential oil isolated from leaves of H. scabrum collected in Bolivia and Peru has previously been investigated; however, the chemical compositions of the essential oils from male and female individuals were not compared.…”

Phytochemical analysis of the hot tea infusion of Hedyosmum brasiliense

“…It is employed in folk medicine irrespective of its androecious or gynoecious parts, in the form of tea or brewed in white wine as a sedative, anti-inflammatory, diuretic, aphrodisiac and also for the treatment of migraines and diseases of the ovary as well as a substitute for green tea (Di Stasi et al, 1988;Reitz, 1965). Reported pharmacological or biological information indicate that the ethanol extract, the essential oil and sesquiterpene lactones from H. brasiliense possess antinociceptive (Trentin et al, 1999), antimicrobial (Kirchner et al, 2010) and neurochemical properties (Goncalves et al, 2012;Tolardo et al, 2010). Recently, some sesquiterpene lactones and phenolic compounds from fractions of the ethanol extract have been isolated (Amoah et al, 2013).…”

“…This study reports the isolation and identification of nine secondary metabolites from HB, one of them is a new sesquiterpene lactone, together with known phenolic acids, flavonoids and neolignans. Considering the previous reported antimicrobial activity of HB (Kirchner et al, 2010) the antimycobacterial activity of the aqueous extract and its main compound was also investigated in this work.…”