Chemical composition and bioactive properties of the lichen, &lt;i&gt;Pleurosticta acetabulum&lt;/i &gt;

Tomović, Jovica; Kosanić, Marijana; Ristić, Svetlana; Ranković, Branislav; Stanojković, Tatjana; Manojlović, N.

doi:10.4314/tjpr.v16i12.23

Cited by 15 publications

(14 citation statements)

References 14 publications

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“…In the investigated extracts, depsidones and depsides were present as the most abundant substances classes. Comparing the methanol lichen extracts with our earlier study of the acetone extracts of P. acetаbulum and C. subulata, the presence of the same metabolites was identified, but signal intensities and areas below the absorption maximum of certain secondary metabolites differed, which is consistent with the ability of the solvent (acetone and methanol) to dissolve more or less of these metabolites, by the principle of "dissolve similarly" (11)(12). Such data indicate the influence of solvents of different polarity on the extraction of individual components from the samples (22).…”

Section: Discussionsupporting

confidence: 71%

“…The column was thermostated at the temperature of 300 0 C. Identification of individual constituents of the extracts was made by comparing the retention times (tR) and UV spectra of constituents with standards (λ =200-400 nm). Used standards were obtained from the following sources: hypoprotocetraric acid is isolated from Cladonia pyxidata, fumarprotocetraric acid is isolated from Hypogymnia physodes, salazinic acid isolated from the Lobaria pulmonaria, norstictic acid from the lichen Ramalina farinacea, protocetraric acid is isolated from Toninia Candida, and evernic acid is isolated from the lichen Evernia prunastri (11)(12).…”

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

“…The aim of the present study was to identify the secondary metabolites of Cladonia subulata and Pleurosticta acetabulum from Serbia using HPLC-UV analysis and to investigate in vitro antioxidant and anticancer activities of the methanol extract from these lichens. Also, the aim of the study is to compare the influence of solvents of different polarities on the extraction of individual components from the samples with our earlier studies (11)(12).…”

Section: Introductionmentioning

confidence: 99%

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Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Tomović

Kosanić

Ranković

et al. 2023

Experimental and Applied Biomedical Research (EABR)

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The aim of this study is to investigate the chemical composition of methanol extracts of the lichens Pleurosticta acetabulum and Cladonia subulata and their antioxidant, and anticancer activities. The phytochemical analysis of the extracts of lichens was determined by HPLC-UV method. The predominant phenolic compounds in these extracts were norstictic acid and salazinic acids in P. acetabulum, while hypoprotocetraric acid and fumarprotocetraric acid were the major metabolites detected in C. subulata. Total phenolics and flavonoids in the extracts were determined spectrophotometrically, with the varied amount from 21.31 to 73.45 mg GA/g and from 8.48 to 15.42 mg RU/g, respectively. The lichen extracts showed comparable and strong antioxidant activity, exhibited higher DPPH and hydroxyl radical scavengings, inhibitory activity towards lipid peroxidation and reducing power. Cytotoxic effects of lichens were tested against HeLa S3 and LS174 cell lines using MTT method.The cytotoxic effects of P. acetabulum and C. subulata extracts toward two cancer cell lines were in the range from 39.17 to >200 μg/mL IC50 value. The present study showed that the tested extracts of lichens demonstrated important antioxidant and anticancer effects. That suggests that these lichens can be used as new sources of the natural antioxidants and anti-cancer compounds.

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Section: Discussionsupporting

confidence: 71%

Section: High-performance Liquid Chromatography (Hplc) Analysismentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Tomović

Kosanić

Ranković

et al. 2023

Experimental and Applied Biomedical Research (EABR)

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“…It appeared that flavonoids and phenols are mainly responsible for the antioxidant activity of the examined extracts [ 265 , 266 , 267 , 268 ]. Some of them also exhibited beneficial antimicrobial and anticancer activities [ 269 , 270 ].…”

Section: Antioxidant Capacity Of Extracts From Natural Sourcesmentioning

confidence: 99%

Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles

et al. 2021

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Natural extracts are the source of many antioxidant substances. They have proven useful not only as supplements preventing diseases caused by oxidative stress and food additives preventing oxidation but also as system components for the production of metallic nanoparticles by the so-called green synthesis. This is important given the drastically increased demand for nanomaterials in biomedical fields. The source of ecological technology for producing nanoparticles can be plants or microorganisms (yeast, algae, cyanobacteria, fungi, and bacteria). This review presents recently published research on the green synthesis of nanoparticles. The conditions of biosynthesis and possible mechanisms of nanoparticle formation with the participation of bacteria are presented. The potential of natural extracts for biogenic synthesis depends on the content of reducing substances. The assessment of the antioxidant activity of extracts as multicomponent mixtures is still a challenge for analytical chemistry. There is still no universal test for measuring total antioxidant capacity (TAC). There are many in vitro chemical tests that quantify the antioxidant scavenging activity of free radicals and their ability to chelate metals and that reduce free radical damage. This paper presents the classification of antioxidants and non-enzymatic methods of testing antioxidant capacity in vitro, with particular emphasis on methods based on nanoparticles. Examples of recent studies on the antioxidant activity of natural extracts obtained from different species such as plants, fungi, bacteria, algae, lichens, actinomycetes were collected, giving evaluation methods, reference antioxidants, and details on the preparation of extracts.

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“…Lichens are naturally produced from the symbiotic associations between algae and fungi [1]. Compounds originating from lichen extracts have attracted attention due to their promising biopharmaceutical activities such as antiulcerogenic, anti-inflammatory, anticancer, immunostimulating, antioxidant, antipyretic, analgesic, and antimicrobial capabilities [2][3][4][5][6]. However, few studies were reported on the genotoxic as well as antigenotoxic activities of lichens.…”

Section: Introductionmentioning

confidence: 99%

In Vitro Antigenotoxic, Antihelminthic and Antioxidant Potentials Based on the Extracted Metabolites from Lichen, Candelariella vitellina

et al. 2020

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Lichens have recently received great attention due to their pharmacological potentials. The antigenotoxic potential of C. vitellina extract (25 and 50 µg/mL) was assessed in normal human peripheral blood lymphocytes (HPBL) against Mitomycin C (MMC) co-treatments. Flow cytometric analyses of cell cycle distribution, as well as apoptosis (Annexin V/PI), revealed that the extract had significantly (p ≤ 0.05) ameliorated the MMC toxicity by reducing the apoptotic cells and normalized the cell cycle phases. C. vitellina exhibited antigenotoxicity by ameliorating the diminished mitotic index and DNA single-strand breaks caused by MMC. Herein, the hydromethanolic extract (80%) of Candelariella vitellina (Japan) lichen, exhibited very low cytotoxicity towards normal human peripheral lymphocytes (HPBL) with IC50 >1000 µg/mL. In order to explore the antihelminthic effect, Echinococcus granulosus protoscoleces were used in vitro. Eosin staining revealed significant (p ≤ 0.05) dose and time-dependent scolicidal effects of the extract confirmed by degenerative alterations as observed by electron scan microscopy. Furthermore, primary and secondary metabolites were investigated using GC-MS and qualitative HPLC, revealing the presence of sugars, alcohols, different phenolic acids and light flavonoids. Significant antioxidant capacities were also demonstrated by DPPH radical-scavenging assay. In conclusion, the promising antigenotoxic, antihelminthic and antioxidant potentials of C. vitellina extract encourage further studies to evaluate its possible therapeutic potency.

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Chemical composition and bioactive properties of the lichen, <i>Pleurosticta acetabulum</i >

Cited by 15 publications

References 14 publications

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Characteristics, Chemical Analysis and Biological Activities of Methanol Extracts of Lichens Pleurosticta Acetabulum and Cladonia Subulata

Antioxidants: Classification, Natural Sources, Activity/Capacity Measurements, and Usefulness for the Synthesis of Nanoparticles

In Vitro Antigenotoxic, Antihelminthic and Antioxidant Potentials Based on the Extracted Metabolites from Lichen, Candelariella vitellina

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