Chemical Composition and in Vitro Antifungal Activity Screening of the Allium ursinum L. (Liliaceae)

Abstract

The objective of the study was to summarize the methods for isolating and identifying natural sulfur compounds from Allium ursinum (ramson) and to discuss the active constituents with regard to antifungal action. Using chromatographic techniques, the active constituents were isolated and subsequently identified. Analyses by high-performance liquid chromatography (HPLC) suggested that these compounds were sulfur constituents, with a characteristic absorbance at 250 nm. Gas chromatography-mass spectrometry (GC-MS) analyses allowed the chemical structures of the isolated constituents to be postulated. We adopted the same methods to identify the health-giving profiling of ramsons and the effects are thought to be primarily derived from the presence and breakdown of the alk(en)ylcysteine sulphoxide, alliin and its subsequent breakdown to allicin (sulfur-compounds of ramson) in connection with antifungal action. The aim of the study was the characterization of the chemical composition of ramsons and the testing of the action of the in vitro extracts, on different strains of Candida albicans. The main goal was to highlight the most efficient extracts of Allium ursinum that can provide long-term antifungal activity without remissions. The extracts from Allium ursinum plants, inhibited growth of Candida spp. cells at concentrations ranging from 0.5 to 4.0 mg/mL, while that of adherent cells at concentrations ranging from 1.0 to > 4.0 mg/mL, depending on the yeast and plant species.

REACTION OF PARENTAL LINES OF Ruse 555 MAIZE HYBRID TO THE AGRO–METEOROLOGICAL CONDITIONS OF IASS “OBARZTSOV CHIFLIK”–ROUSSE

“…It is a variety trait and can be considered only within the variety [KALOSHINA, 1973, BAGIU, et al, 2012.…”

“…Deviations of some factors of the environment beyond the optimal ones in which the ontogenesis is performed leads to disturbance of their physiological activity and biological regime [TOMOV, 1990;VULCHINKOVA et al, 2000BUTNARIU et al, 2012 reported that maize hybrids as heterozygous organisms were more tolerant to the adverse climatic conditions than lines as homozygous ones [BUTNARIU et al, 2012, BOSTAN et al, 2013 .…”

Study on the Response of Alfalfa Germplasm for Resistance to Root Rot

“…Health of the roots was estimated by cutting the root at 10 cm below the root neck, according to the Scale of Lubenets and [SHTOUKINA, 1968, BUTNARIU, et al, 2008, BAGIU, et al, 2012 based on changes that have occurred in the color of the root vascular system.…”

Forage Productivity and Weed Infestation of Annual Early– Spring Mixture Under Different Sowing Dates

“…According to the obtained yields in the other later sowing periods the excess was in the range of 7.23% (compared to var. 2 The analysis of weed infestation of early spring mixed crop reported in Fig. 1 shows that in three years, regardless of the sowing time is relatively weak and percentage shares of the components (spring field pea and oats) is more than the weeds.…”

“…For components cereals are used oats, barley and by legumes-pea, narrow-leaved vetch, grass pea vine and others [PUTNOKY et al, 2013, BAGIU et al, 2012, RATA et al, 2012 .…”