Chemical Composition and Physiological Activity of Opuntia ficus-indica depending on Different Cultivation Regions

Abstract

This study was conducted to investigate changes in the proximate composition, antioxidant activities, and α-glucosidase inhibitory activity of Opuntia ficus-indica (OFI) cladodes cultivated in Jeju (JJ1, JJ2, JJ3) and Jeonnam (JN1, JN2). The difference in the proximate composition (crude protein, lipid and ash content) of OFI between the two regions was not significant. Ca, Mg and Na were the major mineral components of OFI. The ascorbic acid content of OFI ranged from 57.87 to 143.72 mg/100 g. A 70% ethanol extract was used to investigate the antioxidant content and activity as well as the α-glucosidase inhibitory activity. The total polyphenol and flavonoid contents of OFI were 38.69~55.29 and 3.33~4.03 mg/g, respectively. The antioxidant activities based on the DPPH and ABTS free radical scavenging assays were 45.19~ 61.52% and 39.15~51.96%, respectively, at a concentration of 1 mg/mL. The inhibitory activity of OFI extracts against rat intestinal α-glucosidase was 29.72~45.73% at 1 mg/mL concentration, and JN1 showed the highest α-glucosidase inhibitory activity. This information could be very useful for authentication of Opuntia species with the highest potential as sources of nutritional and therapeutic elements.

Quality and Characteristics of Scone Added with Pinus koraiensis Leaf Powder

“…To measure the flavonoid content, the method of Lee et al (1997) was applied. After adding 1 mL of diethylene glycol to 0.1 mL of sample, 0.1 mL of 1 N NaOH was added, mixed, and reacted at room temperature for 1 h, then the absorbance was measured at 420 nm.…”

What Is the Relationship between Antioxidant Efficacy, Functional Composition, and Genetic Characteristics in Comparing Soybean Resources by Year?

“…Subsequently, after the reagent reacted with the extract and was set aside for 30 min, absorbance was measured at 734 nm. In this case, L-ascorbic acid was used as a control [ 10 , 24 ].…”

“…First, 0.2 mM of DPPH (Sigma-Aldrich, St. Louis, MO, USA) solution was prepared in methanol, reacted with the extract, and set aside for 30 min. Subsequently, absorbance was measured at 734 nm with L-ascorbic acid (Sigma-Aldrich, St. Louis, MO, USA) as a control [ 10 , 24 ].…”

A Correlation Study on In Vitro Physiological Activities of Soybean Cultivars, 19 Individual Isoflavone Derivatives, and Genetic Characteristics

“…DPPH radical scavenging activity was measured as follows: 0.2 mM 1,1-diphenyl-2-picrylhydrazyl (DPPH, Sigma-Aldrich, St. Louis, USA) was prepared in methanol and reacted with the soybean extract at a final concentration of 500 µg/mL. After 30 min, absorbance was measured at 520 nm [ 13 ]. Ascorbic acid diluents (0–1000 µg/mL) were used to draw a calibration curve, and the result was calculated as the ascorbic acid equivalent (mg) per g of freeze-dried soybean seed weight (mg AA eq/g) [ 1 ].…”

“…ABTS Radical Scavenging Activity ABTS radical scavenging activity was measured as follows: 7.4 mM 2,2 -azino-bis-3ethylbenzo-thiazoline-6-sulfonic acid (ABTS, Sigma-Aldrich, St. Louis, USA) and 2.6 mM potassium persulfate (Sigma-Aldrich, St. Louis, MO, USA) were prepared, and stirred for 24 h to form ABTS cations. Subsequently, the reagent was allowed to react with the soybean extract at a final concentration of 500 µg/mL for 30 min, and then absorbance was measured at 734 nm [13]. Ascorbic acid diluents (0-1000 µg/mL) were used to draw a calibration curve, and the result was calculated as the ascorbic acid equivalent (mg) per g of freeze-dried soybean seed weight (mg AA eq/g) [1].…”