Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery

Mahmoudvand, Hossein; Kheirandish, Farnaz; Dezaki, Ebrahim Saedi; Shamsaddini, Saeedeh; Harandi, Majid Fasihi

doi:10.1016/j.biopha.2016.05.012

Cited by 35 publications

(25 citation statements)

References 29 publications

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“…To measure clinical chemistry parameters in serum, blood was collected into tubes containing no anticoagulant, allowed to clot, and serum was separated by centrifugation at 2000g for 20 min. The assays of aspartate aminotransferase (AST), alanine aminotransferase (ALT), alkaline phosphatase (ALP), creatinine (Cr), blood urea nitrogen (BUN), and bilirubin (direct and total), were performed using Roche diagnostics kits (Mannheim, Germany) (19,20).…”

Section: Sub-acute Toxicitymentioning

confidence: 99%

Chemical composition, acute and sub-acute toxicity of Satureja khuzestanica essential oil in mice

Fallahi¹,

Beyranvand²,

Mahmoudvand³

et al. 2017

Marmara Pharmaceutical Journal

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To date, herbal medicines are the important source in the development of new drugs to remedy various diseases. However, it is crucial to provide scientific justification of these drugs to determine their side effects in treatments. This study was aimed to evaluate the chemical composition, acute and subacute toxicity of Satureja khuzestanica essential oil (SKEO) in a mice model. Plant materials of S. khuzestanica were collected from the rural regions of Lorestan Province, west of Iran. Gas chromatography/mass spectrometry (GC/MS) analysis was performed to determine the main components of SKEO. To assess the acute toxicity, 0.5, 1, 2 and 4 ml/kg doses of SKEO were injected intraperitoneally into four groups of six mice. The number of deaths was counted at 24 h after the treatment. Sub-acute toxicity of the effects of SKEO was evaluated by determine the clinical chemistry and hematological parameters of the treated mice after oral administration of SKEO at the doses of 0.2, 0.4 and 0.6 ml/kg, respectively, for 14 consecutive days. Twenty-three compounds were identified in SKEO by GC/MS analysis, in which the main component was carvacrol (64.4%). The LD50 value of intraperitoneal injection of SKEO was 1.79 ml/kg and the maximum nonfatal dose was 1.13 ml/ kg. No death was observed at the doses of 0.2 and 0.4 ml/kg, while in the group receiving 0.8 ml/kg of SKEO, only one mouse died (12.5%). There was no significant difference between the biochemical and hematological parameters following the oral administrations of SKEO at the used doses of 0.2, 0.4 and 0.8 ml/ kg and the controls (P>0.05). The obtained results in this work showed that SKEO at the tested doses had no significant toxicity on the liver and kidney tissues as well as on the hematological parameters in the mice. Therefore, SKEO could be safe for the mammalian host at the used doses.

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Section: Sub-acute Toxicitymentioning

confidence: 99%

Chemical composition, acute and sub-acute toxicity of Satureja khuzestanica essential oil in mice

Fallahi¹,

Beyranvand²,

Mahmoudvand³

et al. 2017

Marmara Pharmaceutical Journal

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“…Surgery is considered today as one of the most important treatments for hydatidosis. Due to laceration, the cyst and spread of the content within it (protoscoleces) during the surgery that can put the patient at the risk of re-infection, immunological reactions such as anaphylaxis shock and even death, surgeons use some chemical drugs such as hypertonic saline, Ag-nitrate and cetrimide to minimize surgical dangers [ 5 ]. However, recent studies have confirmed severe complications such as necrosis, fibrosis and impaired performance of liver and gallbladder following the use of these agents [ 6 , 7 ].…”

Section: Introductionmentioning

confidence: 99%

In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscolecs during hydatid cyst surgery

Niazi

Saki

Sepahvand

et al. 2019

Annals of Medicine and Surgery

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Background Nowadays, surgery is considered as one of the most important treatments for hydatidosis. Due to laceration, the cyst and spread of the content within it (protoscoleces) during the surgery that can put the patient at the risk of re-infection, anaphylaxis shock and even death, surgeons use some chemical drugs as protoscolicidal agents. The study is aimed to evaluate the scolicidal effects of olive ( Olea europaea L.) leaf extract on hydatid cyst protoscoleces in vitro and ex vivo . Methods After the collection of protoscoleces from sheep livers infected with fertile hydatid cysts, they were treated with various concentrations of olive leave extract (75–300 mg/mL) for 5–30 min in vitro and ex vivo . Finally, the mortality of protoscoleces was assessed by the eosin exclusion test (0.1% eosin staining). Results The mean of the mortality of protoscoleces was 100% after 10 min of incubation with the concentration of 300 mg/ml of O. europaea leaves extract. On the other hand, the mean of the mortality of protoscoleces after 20 min of incubation with the concentration of 150 mg/ml of O. europaea leaves extract was 100%. After injection of O. europaea leaves extract directly into the hydatid cyst ( ex vivo ), the mean of the mortality of protoscoleces was 100% after 12 and 25 min of incubation with the concentration of 300 and 150 mg/ml of O. europaea leaves extract, respectively; indicating that the extract requiring a further time to display a potent protoscolicidal effects. Conclusion Based on the findings of the study, it can be concluded that the extract of olive leaf had a significant scolicidal activity on hydatid cyst protoscoleces. However, further research, especially in human and animal subjects, are required to reach this conclusion.

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“…[ 39 40 ] Previous investigations on laboratory animals have demonstrated that liver and kidney is the main object limb of drug toxicity. [ 41 42 ] Injure to the structural integrity of the liver is evaluated by increased serum levels of enzymes such as ALT, AST, ALP, and bilirubin. On the other hand, the Cr blood test and BUN are used to evaluate kidney function.…”

Section: Discussionmentioning

confidence: 99%

In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice

Mahmoudvand

Oliaee

et al. 2017

Phcog Mag

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Background:This study investigates the scolicidal effects of Cinnamomum zeylanicum essential oil against the protoscoleces of hydatid cysts and its toxicity in the mice model.Materials and Methods:Gas chromatography/mass spectroscopy analyses were used to identify the constituents of essential oil. Protoscoleces were treated with different concentrations of the essential oil (6.25–100 µL/mL) in each test tube for 5–30 min. The viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining). Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oil (0.5–4 mL/kg).Results:The main components were found to be cinnamaldehyde (91.8%), ρ metoxicinamate (1.57%), and α pinene (1.25%). Findings indicate that C. zeylanicum essential oil with the concentrations of 100 and 50 µL/mL killed 100% of protoscoleces after 5 min of exposure. Also, the lower concentrations of C. zeylanicum essential oil motivated a late protoscolicidal effect. The LD50 value of intraperitoneal injection of C. zeylanicum essential oil was 2.07 mL/kg body weight after 48 h, and the maximum nonfatal dose was 1.52 mL/kg body weight. The results also showed that there was no significant toxicity following oral administration of C. zeylanicum essential oil for 2 weeks.Conclusion:The results exhibited the favorable scolicidal activity of C. zeylanicum, which could be applied as a natural scolicidal agent in hydatid cyst surgery.SUMMARY We evaluated the efficacy of Cinnamomum zeylanicum essential oil against hydatid cyst protoscolecesThe viability of protoscoleces was confirmed using eosin exclusion test (0.1% eosin staining)Forty-eight male NMRI mice were also used to determine the toxicity of C. zeylanicum essential oilC. zeylanicum with potent scolicidal activity could be applied as a natural scolicidal agent in surgery. Abbreviations used: GC/MS: Gas chromatography/mass spectrometry analysis; CE: Cystic echinococcosis; LD50: Lethal dose 50%; I.p: Intraperitoneally.

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Chemical composition, efficacy and safety of Pistacia vera (var. Fandoghi) to inactivate protoscoleces during hydatid cyst surgery

Cited by 35 publications

References 29 publications

Chemical composition, acute and sub-acute toxicity of Satureja khuzestanica essential oil in mice

Chemical composition, acute and sub-acute toxicity of Satureja khuzestanica essential oil in mice

In vitro and ex vivo scolicidal effects of Olea europaea L. to inactivate the protoscolecs during hydatid cyst surgery

In vitro protoscolicidal effects of Cinnamomum zeylanicum essential oil and its toxicity in mice

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