Chemical composition of the silver fir (Abies alba) bark extract Abigenol® and its antioxidant activity

Silver fir (Abies alba) trunk extract protects guinea pig arteries from impaired functional responses and morphology due to an atherogenic diet

“…In vitro tests revealed that SFTE is a better hydroxyl radical scavenger than resveratrol, BHT, vitamin E or epigallocatechin gallate. Our previous study evaluated the high ability of SFTE to scavenge free radicals in PBMC cells (Tavčar Benković et al 2014), and those findings were supported by a study by Vasincu et al, who showed that ethyl acetate bark extract effectively scavenged free radicals (DPPH, superoxide anions and hydroxyl radicals) and chelated ferrous ions (Vasincu et al 2013). In general, antioxidants reduce the formation of free radicals and other reactive oxygen species that can directly injure the arterial endothelium and cause lipid peroxidation of LDL particles, which then enter the blood vessel wall.…”

“…Silver fir trunk extract, characterized in our previous studies was prepared by the following two-step process, according to a modification of a previously published procedure (Štrukelj et al 2012;Tavčar Benković et al 2014): 5 kg of the ground trunk of the silver fir (Abies alba Mill., checked with www.theplantlist.org) was extracted with 25 l of water at 70°C for 2 h. The aqueous extract was then evaporated under vacuum to a volume of 5 l. In the second step, the concentrated aqueous extract was extracted with 3 × 3 l of ethyl acetate. Twenty-five milliliters of polyethylene glycol 400 was added to the ethyl acetate extract, and the ethyl acetate was then evaporated from the mixture.…”

“…Their specific analysis was carried out by a validated HPLC method (Tavčar Benković et al 2014). The content of protocatechuic acid was 7.7 g/l and the content of p-coumaric acid was 3.7 g/l.…”

“…SFTE exhibits strong ex vivo antioxidative activity when incubated with primary human peripheral blood mononuclear cells (Tavčar Benković et al 2014); however, no scientific data have been published regarding SFTE's efficiency under physiological conditions. Other studies on Abies alba wood revealed the potency of ethyl acetate extract in scavenging free radicals and chelating ferrous ions (Vasincu et al 2013).…”

Affordable Production of Antioxidant Aqueous Solutions by Hydrodynamic Cavitation Processing of Silver Fir (<em>Abies Alba</em> Mill.) Needles

“…Aqueous extracts from bark of silver fir in Slovenia were tested for in vitro antioxidant activity towards DPPH radicals, in a comparative study with analogous extracts from maritime pine [21].…”