Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones

Kachkoul, Rabie; Housseini, Tarik Squalli; Mohim, Mohamed; Habbani, R. El; Miyah, Youssef; Lahrichi, Anissa

doi:10.1016/j.joim.2019.08.001

Cited by 25 publications

(23 citation statements)

References 54 publications

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“…Briefly, 2 g of the fruit peel powder placed in 100 mL of boiling distilled water is allowed to be infused for 30 min and then filtered using a filter paper with a diameter of 1.6 μm. e infusion (I.PG) was concentrated by Rotavapor and stored in a temperature of − 4°C until use [41,45].…”

Section: Infusionmentioning

confidence: 99%

“…e chemical compounds of E.PG and I.PG have been identified by UPLC in reverse phase using a Waters ACQUITY SDS UPLC system with a Column type: ACQUITY UPLC ® BEH C18 1.7 μm, as followed in the method of Kachkoul et al [45]. In this respect, the separation is carried out in gradient mode using acetonitrile (CH 3 CN) and formic acid (100 : 0.1 v : v) according to the following steps: (1) initial mobile phase including 10% of CH 3 CN, (2) linear increase from 10% to 25% of CH 3 CN (0-18 min), (3) 25% of CH 3 CN (18-21 min), (4) from 25% to 50% of CH 3 CN (21-28 min), (5) from 50% to 100% of CH 3 CN (28-29 min), and (6) 100% of CH 3 CN (29-30 min); then the percentage of CH 3 CN has been reduced to 10% (30-37 min), which allowed a stabilization time of 7 min before injection of the next sample.…”

Section: Uplc-pda-esi-ms Analysismentioning

confidence: 99%

“…After incubation for 30 min in the dark and at room temperature, the absorbance (A) has been measured at λ � 517 nm. e inhibition percentages have been calculated by the following relationship [45]:…”

Section: Uplc-pda-esi-ms Analysismentioning

confidence: 99%

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Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Kachkoul

Mohim

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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e plant Punica granatum L. has several biological activities and a great curative and preventive power against chronic diseases. For this purpose, the objective of this work is to valorize the fruit peel of this plant in the field of phytomedicine, by quantifying and identifying its bioactive compounds and by evaluating their antioxidant and anticrystallization activities against calcium oxalate. is comparative study has been carried out by hydroalcoholic extract (E.PG) and infusion (I.PG) of the plant. e quantification of the phenolic compounds has been performed by spectrophotometric methods, and the chemical species identification has been performed by UPLC-PDA-ESI-MS. Moreover, the examination of the antioxidant activity has been executed by both methods of DPPH and FRAP. e crystallization inhibition has been studied in vitro by the turbidimetric model. e characterization of the synthesized crystals has been accomplished by microscopic observation and by Fourier Transform Infrared Spectroscopy. e results found show the comparable importance of the two plant extracts in the elimination of free radicals; the values of the half maximal inhibitory concentration "IC 50 " obtained are in the order of 60.87 ± 0.27 and 59.91 ± 0.83 μg/mL by the DPPH method and in the order of 42.17 ± 7.46 and 79.77 ± 6.91 μg/mL by the FRAP method, for both E.PG and I.PG, respectively. Furthermore, the inhibition percentages of calcium oxalate crystallization are in the range of 98.11 ± 0.17 and 98.22 ± 0.71% against the nucleation and in the order of 88.98 ± 0.98 and 88.78 ± 2.48% against the aggregation, for E.PG and I.PG, respectively. ese results prove the richness of the plant in bioactive compounds, offering an antioxidant and anticrystallization capacity; therefore, it can be used in the treatment and/or the prevention of stone formation.

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Section: Infusionmentioning

confidence: 99%

Section: Uplc-pda-esi-ms Analysismentioning

confidence: 99%

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Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Kachkoul

Mohim

et al. 2020

Evidence-Based Complementary and Alternative Medicine

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“…We evaluated the effectiveness of the saponins fraction in vitro on CaOx dissolution using the method illustrated by Yachi et al [21] and Kachkoul et al, [22] with a slight modification.…”

Section: Ability Of the Saponins Fraction To Dissolve The Caox Precipmentioning

confidence: 99%

Untitled

Ahmed¹

2020

IJGP

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Introduction: A large number of medicinal plants have been used in Algeria since antiquity, which claims the efficient cure of urinary stone, one of these plants is the specie Zizyphus lotus (Family: Rhamnaceae). Leaves of this species are traditionally used for the treatment of many diseases. Nowadays, numerous clinical studies have provided reliable results on the effects of several hundred plants on several diseases such as the problem of urinary stones. The objective of this study is evaluated in vitro the activity antilithiasic of the extract saponins created fraction. Materials and Methods: Inhibition and dissolution of calcium oxalate (CaOx) were studied by the use of the techniques turbidimetric and gravimetric, using a ultraviolet-visible spectrophotometer and a precision balance. Results and Discussion: Qualitative secondary metabolites determination in the leaves of Z. lotus showed the presence of polyphenols, saponins, flavonoids, alkaloids, and tannins, and the yield of the fraction rich in saponins is equal to 1.41%. The absorbance decreased with the increase in the concentration of extract indicating that decreased the nucleation of CaOx particles with inhibition at 55.23 ± 1.23%. The weight of CaOx crystal decreased with the increase in the concentration of saponins, and the extract at 100% concentration possesses a maximum percentage of dissolution equal to 91.95 ± 0.72%. This effect could be attributed to Ca 2+ chelation by the compounds that exist in this fraction. Conclusion: This study indicates that extract can be considered as feasible natural products to improve the efficiency of CaOx inhibition and dissolution.

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“…3 m in height and in the winter blooms with pinkish-white flowers. The fleshy fruit is often consumed as a jam or used in the manufacture of digestive and/or laxative liqueurs [2][3][4][5]. Recent reports point to a role of A. unedo extracts in treating type-2 diabetes, by inhibiting α-amylase activity.…”

Section: Introductionmentioning

confidence: 99%

Moroccan Strawberry Tree (Arbutus unedo L.) Fruits: Nutritional Value and Mineral Composition

lhaj

Bchitou

Gaboun³

et al. 2021

Foods

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The strawberry tree (Arbutus unedo L.), grown throughout the Mediterranean, produces edible fruit; as it is easily bruised, the sweet, reddish fruit is used mostly to prepare jams, marmalades and alcoholic beverages. As the genus is paraphyletic, phytochemical analysis can assist in defining the fruit composition with the species Arbutus unedo L. (A. unedo). Here we report on the carbohydrate, total sugar, protein, fat, fiber, ash, and mineral content of wild fruit, harvested from 45 specimens from five locations. The dominant nutrients were carbohydrates (78.2–84.8 g/100 g), total sugars (52.1–67.2 g/100 g) and dietary fiber (11.0–20.1 g/100 g). Other important nutrients supplied by A. unedo fruit include P, K, and Fe. The fruit was observed to contain health-promoting components providing 42 and 36%, of recommended daily allowance (RDA) for fiber and zinc, respectively, as well as iron and manganese, at levels exceeding minimum RDA. The free-sugar profile revealed high glucose followed by fructose content with minor amounts of sucrose (14, 11, and 6 g/100 g, respectively). Significant differences both between regions and within individuals were observed for several traits. The richness of fruit nutrients in A. unedo confers nutritional value and as such, a promising alternative fruit source.

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Chemical compounds as well as antioxidant and litholytic activities of Arbutus unedo L. leaves against calcium oxalate stones

Cited by 25 publications

References 54 publications

Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

Chemical Compounds Identification and Antioxidant and Calcium Oxalate Anticrystallization Activities of Punica granatum L.

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Moroccan Strawberry Tree (Arbutus unedo L.) Fruits: Nutritional Value and Mineral Composition

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