Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides

Axén, Rolf; Porath, Jerker; Ernback, Sverker

doi:10.1038/2141302a0

Cited by 2,095 publications

(451 citation statements)

References 14 publications

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“…Purification of serum and antibodies was carried out as follows: azurin (3 mg) was coupled to l g of CNBr-activated Sepharose 4B [16]. Anti-azurin serum (2 ml) was diluted with 2 x PBS-Tween to 4 ml and loaded on the gel.…”

Section: Immunological Techniquesmentioning

confidence: 99%

Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli

et al. 1989

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The structural gene for the blue copper protein azurin from Pseudomonas aeruginosa has been subcloned in different expression plasmid vectors. The highest yield of expression was obtained when the gene with its native ribosome-binding site was placed downstream of the lac promoter in plasmid pUCI8. The protein is exported to the periplasmic space in Escherichia coli and the amount corresponds to 27% of the total protein content in the periplasmic space. The preprotein is cleaved correctly according to N-terminal sequencing of the purified protein. Azurin has been purified in large amounts and is spectroscopically indistinguishable from the protein purified from P. aeruginosa.

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Section: Immunological Techniquesmentioning

confidence: 99%

Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli

et al. 1989

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“…Cofactors were coupled directly to cellulose by the cyanogen bromide technique described by Axen, Porath and Ernblck [4] as follows: cellulose (20 g) was washed with distilled water and suspended in about 50 ml water. Cyanogen bromide (6 g) was added and the pH maintained at pH 11 + 0.2 by addition of 8 N KOH.…”

Section: Methodsmentioning

confidence: 99%

Affinity chromatography of enzymes on insolubilized cofactors

1971

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“…The RCA, agglutinin (195 mg) was dialyzed 20 h against 0.1 M NaHCOa, 0.5 M NaC1 (pH 8.5). It was mixed with 20 ml CNBractivated Sepharose 4B (Pharmacia) as in [14]. CNBr (Eastman Kodak Co.) was 20 mg/ml in 25 ml.…”

Section: Affinity Chromatography On Lectinsmentioning

confidence: 99%

Kininogen molecular heterogeneity by affinity chromatography on concanavalin A and Ricinus communis lectins

Turpeinen¹,

Syvänen²,

Hamberg³

1981

FEBS Letters

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Chemical Coupling of Peptides and Proteins to Polysaccharides by Means of Cyanogen Halides

Cited by 2,095 publications

References 14 publications

Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli

Expression of the blue copper protein azurin from Pseudomonas aeruginosa in Escherichia coli

Affinity chromatography of enzymes on insolubilized cofactors

Kininogen molecular heterogeneity by affinity chromatography on concanavalin A and Ricinus communis lectins

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